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Bipyramidal crystalline, spheroidal crystalline and amorphous proteins were prepared from following four seeds: white kidney, navy (Phaseolus vulgaris) beans; baby lima, large lima (Phaseolus lunatus) beans. A study of the biological properties of the proteins which exhibit the different microstructures, was carried out. The nature of tryptic inhibitory activity and alpha-amylase inhibitory activity were investigated. All protein showed both trypsin inhibitory and alpha-amylase activities; the kinetics of the alpha-amylase revealed non-competitive mechanism. The crystalline isolates showed lower trypsin inhibitory (TI) and alpha-amylase inhibitory (AI) activities than the amorphous isolates. The extents of tryptic hydrolysis (in vitro) were not related to TI indicating involvement of some other factors, in addition to trypsin inhibitory activity. Electropherograms of SDS-PAGE indicated that the major proteins of P. lunatus beans were more resistant to tryptic hydrolysis than the major fractions of the P. vulgaris. Phytate was found to have an effect on trypsin inhibition as well as on alpha-amylase inhibition; in the latter case, however, phytate complexed to protein was required for the inhibitory effect. There were no relationships between tannin content of the proteins and biological activity. Fractionation of bipyramidal crystalline and amorphous proteins by size exclusion chromatography showed that each isolate contained three fractions having approximate MW of 443,000, 200,000 and 150,000 daltons. The 200,000 MW fraction was a principal fraction. The fraction of 150,000 contained most of the trypsin inhibitory activity, alpha-amylase inhibitory activity was not detected in any of the fractions.

This study was directed at elucidating the structural and functional characteristics of legume protein isolates with different microstructures. / Protein isolates with different microstructures (crystalline and amorphous) were prepared from four varieties of Phaseolus beans; white kidney bean and navy bean (P. vulgaris), and large lima and baby lima beans (P. lunatus). The protein isolates were subjected to tryptic hydrolysis at various time intervals, and the effect of hydrolysis on the functional properties of the protein isolates was studied, using bovine casein as control. Reversed phase HPLC with UV detection was used to generate peptide maps of the tryptic hydrolysates, and electrospray mass spectrometry was used to characterize the peptide profile of the protein hydrolysates. / There was no difference between the susceptibility of the crystalline and amorphous protein isolates to tryptic hydrolysis, however, the hydrolytic products of the crystalline isolates showed higher solubility and functional properties compared with those of the amorphous isolates. Limited hydrolysis markedly improved the functional properties of both crystalline and amorphous protein isolates. The effect of hydrolysis on the functionality of the bean protein isolates was higher compared with casein. In general the hydrolysates of the bean protein isolates showed higher functional properties than casein and its hydrolysates. / Reversed phase HPLC peptide mapping of the tryptic digests and ESI/MS of the RP-HPLC fractions showed structural and compositional difference between crystalline and amorphous protein isolates prepared from the same bean variety. The results also showed that off-line ESI/MS, and ESI/MS/MS of RP-HPLC fractions of the tryptic hydrolysates could be used for the structural characterization of the protein isolates.

Thesis (Ph. D.)--University of Adelaide, Dept. of Agronomy, Waite Agr. Research Inst., 1985. / Includes bibliographical references (leaves [129]-146).

Thesis (Ph. D.)--University of Wyoming, 2005. / Title from PDF title page (viewed on Oct. 10, 2007). Includes bibliographical references (p. 109-122).

Thesis (Ph. D.)--School of Molecular and Microbial Biosciences, Faculty of Science, University of Sydney, 2004. / Chapter headings on separately inserted unnumbered cream coloured leaves. Bibliography: leaves 122-135.

Thesis (Ph. D.)--University of Sydney, 2004. / Title from title screen (viewed 14 May 2008). Submitted in fulfilment of the requirements for the degree of Doctor of Philosophy to the chool of Molecular and Microbial Biosciences, Faculty of Science. Includes bibliographical references. Also available in print form.

Thesis (Ph. D.)--University of Hong Kong, 1997. / Includes bibliographical references (leaf 161-185).

Thesis (Ph. D.)--University of Hong Kong, 1997. / Includes bibliographical references (leaf 159-173).

Thesis--Wisconsin. / Includes bibliographical references (leaves 38-44).