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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Variability in cultured cells of Capsicum Spp

Holden, Peter Richard January 1989 (has links)
No description available.
2

Induction and assessment of plant cell membrane permeability

Watson, L. D. January 1986 (has links)
This thesis describes the isolation, immobilisation and permeabilisation of <i>Digitalis lanata</i> (foxglove) and <i>Nicotiana tabacum</i> (tobacco) plant cells and protoplasts. Protoplasts were isolated from leaves of <i>Digitalis lanata</i> and <i>Nicotiana tabacum</i> and cultured in conditions of varying osmotic potential, illumination and cell density in order to achieve maximum cell stability. The regeneration of cellulose cell walls in <i>Nicotiana tabacum</i> protoplasts could be inhibited by the addition of dilute concentrations of the herbicide 2,6-Dichlorobenzonitrile. At concentrations of 2-10 mg/l in culture medium cell wall regeneration could be prevented for up to 6-8 weeks. Isolated cells and protoplasts were stabilised by entrapment within a beaded agarose support matrix. <i>Nicotiana tabacum</i> protoplasts and cells, immobilised within an agarose beaded support matrix were used to develop a technique to permeabilise and destabilise the cell membranes. Cells or protoplasts pre-loaded with 6-Carboxy-Fluorescein, Neutral Red stains and <SUP>14</SUP>C-Sucrose or <SUP>86</SUP>Rb<SUP></SUP>+ radioactive tracers were employed as markers for cell permeability or leakiness. Efflux or Compartmental analysis was used to determine the influence of various selected permeabilising agents on the integrity and the leakiness of either protoplast or cell membranes. Immobilised protoplasts or cells were subjected to a three - step procedure involving initial loading with <SUP>86</SUP>Rb<SUP></SUP>+ tracer, a membrane permeabilisation step and finally a recovery step. Protoplast membrane stability could not be regained after the recovery step, following a 30 minute period of permeabilisation with 50 mM acetic acid in culture medium. Immobilised cells could, however, regain membrane integrity with good intracellular retention of <SUP>86</SUP>Rb<SUP></SUP>+ tracer ion during the efflux experiment. Thus, immobilised <i>Nicotiana tabacum</i> cells could be made reversibly permeable with the use of specific agents. It is anticipated that such techniques which encourage reversible permeabilisation of immobilised cells have potential in larger scale plant cell culture systems to effect the release of intracellularly stored, useful secondary metabolites. There are also possibilities for the inclusion of exogenous precursors, cofactors and foreign genetic material which would increase compound yield and may produce novel secondary metabolites.
3

Regeneration in tissue and protoplast culture of Sainpaulia ionantha (African violet)

Redway, F. A. January 1986 (has links)
No description available.
4

Production and identification of interspecific potato somatic hybrids

Hamidoghli, Yousef January 1995 (has links)
No description available.
5

Investigation of the nutrient requirements of Pinus caribaea Morelet in vitro

Satchwell, Christa Elizabeth January 1990 (has links)
No description available.
6

An investigation of metabolite release from plant cells in vitro to their surrounding medium

Kirby, Nigel John January 1987 (has links)
No description available.
7

The biotransformation of monoterpenoids by plant cells in axenic culture

Lappin, G. J. January 1986 (has links)
No description available.
8

The preparation of micropropagated plantlets for transplantation

Smith, Elaine Francis January 1990 (has links)
No description available.
9

Micropropagation and low temperature storage of Dieffenbachia

Hudson, G. January 1985 (has links)
No description available.
10

The genetic manipulation of jute (Corchorus) species

Khatun, Asma January 1993 (has links)
No description available.

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