No description available.

Mechanisms of root growth under variable field conditions were investigated by observing corn ( Zea mays L.) root growth and distribution in the field and by observing the influence of soil physical stresses on corn seedling root growth in controlled environments. The field soil was Groseclose silt loam (clayey, mixed, mesic Typic Hapludult). Groseclose A horizon material was used for the growth chamber experiments where corn was grown in a range of aggregate sizes, bulk densities, low and high soil moisture levels, and temperatures. Rooting patterns in the field were altered by drought. Root length density decreased in the dry surface soil and proliferated in the moist subsurface soil. Distribution of roots length densities was skewed. A few samples contained many roots and many samples contained few roots because roots were restricted to interpedal voids. In the growth chamber experiments, roots were not able to penetrate large aggregates and were restricted to interaggregate zones. This tortuous path of root growth led to transitory impedances as roots were deflected around aggregates. Corn roots were able to push small aggregates out of their path. An equation was developed to describe this impedance as a function of aggregate size, root diameter, and deflection angle. Mechanical impedance, oxygen stress, lower temperatures, and moisture stress reduced seedling root elongation to some extent, but the influence of reduced temperature was the most dramatic. At 6 days corn root length at 21°C was 20% of that at 25°C while root length at 17°C was only 5% of that at 25°C. Mechanical impedance and reduced temperatures also increased root diameter. In wet soil, oxygen stress was the most immediate factor affecting root growth, but after 4 days root elongation was stimulated suggesting other unknown factors. Two semi-empirical models were developed. One was based on the exponential growth rate of the root system and the other based on the linear growth rate of each root member. These models accounted for the reduction in root growth rate due to the soil physical stresses. / Ph. D.

published_or_final_version / Botany / Master / Master of Philosophy

The aim of this PhD research was to study multi-element composition in wetland plants from contrasting habitats, including hot springs, temporary wetlands, and metal-rich areas. Triglochin maritima L. (seaside arrowgrass) was chosen for the study because this species is common in alkaline/saline soils and is adapted to diverse habitats. Eleocharis rostellata, Juncus balticus, Salix exigua, S. boothii, and S. wolfii were also included in the study. Field studies and greenhouse experiments were conducted to study the multi-element composition in plants. In the greenhouse experiment the effects of temperature and soil biota on multielement uptake in T. maritima were studied. Root-zone soils and plant samples were analyzed for 32 - 50 elements using inductively coupled plasma OES/MS spectrometry. The expected outcomes from this research were: 1) the development of multi-element fingerprints for T. maritima and other plant species from contrasting habitats, and 2) a better understanding of the effects of temperature and soil biota on multi-element uptake in T. maritima. Habitat specific element concentration patterns in T. maritima were observed; concentrations of Mn, Li, and B were high in plants from hot spring influenced wetlands, whereas Ca, P, Mg, Fe, Sr, Ba, Ti, and Cu were higher in the plants of temporary wetlands. J. balticus and Salix species from mine impacted and uncontaminated sites revealed distinct differences in multi-element fingerprints. J. balticus showed high concentrations of S, K, Mn, Fe, Cu, Al, As, and Cd at contaminated sites compared to un-contaminated sites. Multi-element fingerprints of Salix species showed that S. boothii had higher concentrations of Mn, Fe, Al, and Ti compared to S. exigua and S. wolfii. To our knowledge for the first time the association of mycorrhizal fungus in T. maritima was confirmed, and significant effects of temperature on element concentrations, contents, and their translocation in plants were observed. Generally, the distribution of the total contents of P, Na, Mn, B, Cu, Mo, Li, Sr, Ti, and Cs in both roots and leaves were lower at 40 °C compared to 20 and 30 °C, but their distribution and translocation from root to leaves were higher at 40°C. Even though the biological and physiological functions of Li, Sr, Ba, Rb, and Ti in plants are not fully understood, these elements were substantially taken up by T. maritima, and significant positive correlations of these elements were found with elements that have known biological functions. Overall, concentrations of Ca, P, Mg, Mn, B, Sr, and Ba in T. maritima showed variation due to differences in habitats, temperature, and experimental growing conditions (greenhouse and field condition). Concentration patterns of Na, K, and Zn were species specific and affected by temperature. Li concentrations varied due to habitat differences, growth conditions, and species differences. Future research directions could include: 1) identification of the fungal species associated with T. maritima and studies to elucidate their possible role in survival of T. maritima in the elevated temperature of hot springs, 2) the effects of soil factors, such as salinity and 3) seasonal variation in uptake and translocation, particularly for the less studied elements with yet unrecognized but potential biological functions in plants. / NIH Grant Number P20 RR016471 from the INBRE Program of the National Center for Research Resources / North Dakota State University. Department of Biological Sciences / Robert H. Levis II Cross Ranch Fellowship / North Dakota State University. Environmental and Conservation Sciences program

Thesis (MTech (Agriculture))--Cape Peninsula University of Technology, 2018. / Zinc deficiency on various soil types have been reported in arable soils of sub Saharan Africa (SSA) including South Africa. A pot trial was conducted at the Cape Peninsula University of Technology, Wellington campus to investigate the interaction of different application rates of Zn at various soil pH on the grain yield and quality of spring wheat in a completely randomized factorial design replicated three times. The four soil pH tested were: pHA: 5.1, pHB: 5.6, pHC: 6.1, pHD: 6.6 which correspond to lime application at 0, 0.5, 1 and 1.5 t/ha. Five Zn rates (Zn1: 3.5; Zn2: 4.5; Zn3: 5.5 Zn4: 6.5, and Zn5: 7.5 mg /kg soil which correspond to Zn1: 7; Zn2: 9; Zn3: 11; Zn4: 13 and Zn5: 15 kg /ha) were applied at two (planting and flowering) growth stages. Yield and yield component data collected were analyzed using SAS version 9.2 and means were separated by Duncun’s Multiple Range Test (DMRT). The results showed that grain yield and yield components were significantly affected by lime application pHC (6.1): 1t/ha at planting. Zn application at planting had no significant effect on the grain yield and yield components. However, at flowering, the simultaneous increase of Zn along with increase in lime positively affected grain yield and yield components. Plant analysis showed that at both stages (planting and flowering), Zn application, especially at pH 6.6, significantly increased P, K, Ca, Na, Mg Fe, Cu and B concentrations in wheat grain, but the concentrations of N, Mn, Zn and protein remained unaffected. Zn application had no effect on most nutrients due to the presence of lime. While the absence of lime, Zn4: 6.5mg/kg (corresponding to 13kg/ha) significantly increased the nutrients. In addition, Zn3: 5.5mg/kg (corresponding to 11kg/ha) promoted Zn absorption by grain in all treatments.

In Australia a class of soils known as sodic duplex soils covers approximately 20% of the continent. Their defining characteristic is a sharp texture contrast between the A (or E) and B horizon. The upper B horizon at the point of contact with the E horizon is often highly sodic and of such a high strength that root growth and proliferation, water conductivity, aeration, water storage and water uptake are restricted. Roots growing in these soils rely on channels created by previous roots or cracks arising from shrink– swell forces associated with seasonal wetting and drying. It has been suggested that by increasing the number of these channels in the subsoil, the structure and permeability of the subsoil would be increased as would be the number of preferential pathways for following generation roots. A biological approach for improving soil macroporosity would be to use plants that can grow through that hostile layer creating new channels. This is known as the primer plant concept. This concept is based on a better understanding of root soil interactions. It is accepted that root growth is influenced by the soil structure and the soil structure is influenced by root growth. However, a lot of these dynamics are still unknown. This project aims to contribute to improving that knowledge by investigating the use of modern techniques to study plant/root interactions in duplex soils. First macroporosity and mesoporosity were characterized in three dimensions using medical computer tomography and micro-tomography. Then the imaging methodology was improved by using a local and adaptive threshold technique based on indicator kriging instead of a global threshold. Using this new methodology, changes in porosity were analysed in intact samples when three different plant species were grown for 12 weeks. The plants were canola (Brassica napus); lucerne (Medicago sativum) and saltbush (Atriplex nummularia) hypothesizing saltbush would change the porosity more because it is a native plant species based on the primer plant concept. The results showed that the porosity changed significantly after root growth but no ii differences were found between plant species. The changes could also not all be attributed to root growth because cracks were also formed after 12 weeks. Therefore, the living roots were visualized and characterized using a new tracing algorithm 'rootviz'. This revealed that saltbush was growing more roots down through the profile. Lucerne seemed to grow roots down the profile as well but to a lesser extend. Both of these plants seemed to have more geotropic features than canola that seemed to grow more laterals and had a more exploratory behaviour.

Winter wheat is commonly grown in rotation with leguminous and non-leguminous crops in the Willamette Valley. For agronomic, economic, and environmental reasons it is important to understand the influence of previous crops on availability of N and other nutrients. Objectives of this study were: (1) to evaluate the effects of long-term rotations on winter wheat response to N fertilizer, and (2) to evaluate the use of Plant Root Simulator���(PRS) probes for measuring soil N mineralization and N availability to winter wheat. Field experiments were conducted over three growing seasons in plots of `Stephens' soft white winter wheat at Hyslop farm. Plots receiving 0, 50, 100, 150 and 200 kg N ha����� at Feekes GS 4 were sampled to determine above ground N uptake, grain yield, and grain protein. In spring 1998, PRS probes were placed in 0 kg N ha����� plots and removed at one-week or two-week intervals. In autumn 1998, probes were placed in unfertilized plots and removed at 1-week, 4-week, and 8-week intervals. Probes measured the availability of NH������-N, NO������-N, K���, Ca�����, Mg�����, and P0��������-P. Grain yield and N uptake were greater for wheat following clover as compared to following oats. Three-year average fertilizer equivalent values calculated from N uptake and grain yield data were 44.5 kg N h����� and 49.0 kg N h�����, respectively. The similarity of these independent measurements suggest that differences in N availability were the primary reason for the rotation effect. PRS probes also detected rotational differences in N availability. Average N recovered by probes sampled at 1-week intervals indicated that there was 63% as much NO������-N available to wheat following oat as compared to clover. Wheat recovered 64% as much N following oats as compared to clover. This suggests that PRS probes are an effective method for predicting relative amounts of plant available N. PRS probes also detected rotational differences in plant available potassium. Agronomic responses are useful for assessing the availability of nutrients that are limiting plant growth. PRS probes, on the other hand, are effective for assessing the availability of both limiting and non-limiting nutrients. / Graduation date: 2000

No description available.

The increasing human population, industrialization, urbanisation and climate change challenges have resulted in an increased demand for already scarce water resources. This has left the agricultural sector with less water for production. Sustainable water management strategies would therefore require accurate determination of water-use. In agriculture, water-use can best be determined from total evaporation which is the loss of water from soil and vegetation to the atmosphere. Accurate quantification of total evaporation from vegetation would require a thorough understanding of water transport processes between vegetation and the atmosphere, especially in a water-scarce country like South Africa. Several methods for estimating total evaporation have been developed and are in use today. Some of the common methods used today are: the Bowen ratio energy balance, eddy covariance, scintillometry, flux variance and surface renewal. However, various methods have advantages and disadvantages. Considerations include the cost of equipment and level of skill required for use of some of the methods. A number of methods involve indirect or direct estimation of sensible heat flux then calculating latent energy flux and hence total evaporation as a residual of the shortened energy balance equation. The main objective of this study is to determine the effects of grassland management practices on the energy balance components as well as on the surface radiation balance. Eddy covariance and surface renewal methods were employed to investigate the effects of grassland management practices (mowing and burning) on the micrometeorology of naturally occurring grassland. A 4.5-ha grassland site (Ukulinga, Pietermaritzburg, South Africa) was divided into two halves: one area was initially mowed (cut-grass site) to a height of 0.1 m while the other was not mowed (tall-grass site). The tall-grass site was later treated by burning and hence referred to as the burnt-grass site. Two eddy covariance systems were deployed, one at each of the cut-grass and the tall-grass sites. The systems each comprised a three-dimensional sonic anemometer to measure high frequency sonic temperature, orthogonal wind speeds and directions and the eddy covariance sensible heat flux (W m-2). Latent energy flux, from which total evaporation was then determined, was calculated as a residual from the shortened energy balance equation from measurements of sensible heat flux, net irradiance and soil heat flux assuming closure is met. Other microclimatic measurements of soil water content, soil temperature, surface reflection coefficient and reflected solar irradiance were performed, the latter with a four-component net radiometer. An automatic weather station was also set up at the research site for continuous measurements of solar irradiance, air temperature, relative humidity, wind speed and direction and rainfall. Water vapour pressure and grass reference evaporation were also determined online. Energy fluxes from the tall-grass site were measured from March to June 2008. Greater total evaporation rates (2.27 mm day-1) were observed at the beginning of the experiment (March). As winter approached most of the energy balance components showed a constant decreasing trend and the average total evaporation rates for May and June were 1.03 and 0.62 mm day-1, respectively. The tall-grass site had consistently lower soil temperatures that changed diurnally when compared to the cut-grass site. The soil water content at both sites showed no significant differences. Most of the energy balance components were similar between the two sites and changed diurnally. Although there were small differences observed between other energy balance components, for example, latent energy flux was slightly greater for the tall-grass site than for the cut-grass site. The tall-grass site had more basal cover and this may have contributed to the differences in temperature regimes observed between the two sites. However, the plants growing at the cut-grass site showed more vigour than the ones at the tall-grass site as spring approached. Burning of a mixed grassland surface caused significant changes to most of the optical properties and energy fluxes of the surface. Following burning, the soil temperature was elevated to noticeable levels due to removal of basal cover by burning. The surface reflection coefficient measured before and after the burn also presented a remarkable change. The surface reflection coefficient was significantly reduced after the burn but a progressive increase was observed as the burnt grass recovered after the spell of spring rains. The energy fluxes: net irradiance, latent energy flux and soil heat flux also increased following the burn but the latent energy flux was reduced as transpiration was effectively eliminated by the burning of all actively transpiring leaves. As a result, the main process that contributed towards latent energy flux was soil evaporation. An ideal surface renewal analysis model based on two air temperature structure functions was used to estimate sensible heat flux over natural grassland treated by mowing. Two air temperature lag times r (0.4 and 0.8 s) were used when computing the air temperature structure functions online. The surface renewal sensible heat fluxes were computed using an iteration process in Excel. The fluxes, obtained using an iterative procedure, were calibrated to determine the surface renewal weighting factor (a) and then validated against the eddy covariance method using different data sets for unstable conditions during 2008. The latent energy flux was computed as a residual from the shortened energy balance equation. The surface renewal weighting factor was determined for each of the two heights and two lag times for each measurement height (z) above the soil surface. The a values obtained during the surface renewal calibration period (day of year 223 to 242, 2008) ranged from 1.90 to 2.26 for measurement height 0.7 m and r = 0.4 and 0.8 s. For a measurement height of 1.2 m and r = 0.4 and 0.8 s, a values of 0.71 and 1.01 were obtained, respectively. Good agreement between surface renewal sensible heat flux and eddy covariance sensible heat flux was obtained at a height of 1.2 m using a = 0.71 and a lag time of 0.4 s. Total evaporation for the surface renewal method was compared against the eddy covariance method. The surface renewal method, for a height of 1.2 m and a lag time of 0.4 s, yielded 1.67 mm while the eddy covariance method yielded 1.57 mm for a typical cloudless day. For the same day for a measurement height of 1.2 m and a lag time of 0.8 s, eddy covariance and surface renewal methods yielded 1.57 and 1.10 mm, respectively. For a lag time of 0.4 s, the surface renewal method overestimated total evaporation by 0.10 mm while for a lag time of 0.8 s, the total evaporation was underestimated by 0.47 mm. As a result, the surface renewal method performed better for z = 1.2 m and a lag time of 0.4 s. The eddy covariance method gave reliable sensible heat fluxes throughout the experiment and this allowed a comparison of fluxes across all treatment areas to be achieved. The short-term analysis of the surface renewal method also gave reliable energy fluxes after calibration. Compared to the eddy covariance method, the surface renewal method is more attractive in the sense that it is easy to operate and use and it is relatively cheap. However, the surface renewal method requires calibration and validation against a standard method such as the eddy covariance method. This study showed that grassland management practices had a considerable effect on surface radiation and energy balance of the mowed and burnt treatment sites. Total evaporation was mainly controlled by the available energy flux, rainfall and grassland surface structure. High total evaporation values were observed during summer when net irradiance was at its highest and grass growth at its peak. Low total evaporation values were observed in winter (dry atmospheric conditions) when net irradiance was at its lowest and most vegetation was dormant. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2011.

[Truncated abstract] A greater adoption of stubble retention, minimum-till and no-till farming practices for the purposes of conserving soil, water and fertility requires a greater understanding of the complexity of physical and chemical interactions between the soil and crop residues. There is currently insufficient knowledge to allow reliable predictions of the effects of different residue types in different environments on soil fertility and crop growth, owing to the many residue characteristics and environmental interactions that have been shown to affect decomposition or nutrient release. The role of fibre and nutrient composition in nutrient release from crop residues, and implications for residue management techniques, were studied. Canola, lupin and field pea residues, obtained from farmland in Meckering and Northam, Western Australia, were separated into upper and basal stems, leaves, and siliques or pods. This was done to provide materials with a wide range of chemical and physical characteristics, and also allowed consideration of differential residue management of plant organs, such as comparing harvested canola siliques and retained canola stubble. Pre-treatment by chopping and/or humidification was applied to residues to provide some information about the processes of nutrient release. Residues were subjected to simulated rainfall to assess nutrient leaching from plant material, and placed on soil in pots in constant-temperature glasshouse conditions to assess decomposition. Amounts and rates of change of residue fibre and nutrients were determined throughout leaching and decomposition. Energy Dispersive X-ray (EDX) microanalysis was used to assess the location of diffusible ions in air-dried residues and the effects of humidification on nutrient positioning and release. ... However, the release of calcium and magnesium depended on the decomposition of the more recalcitrant components such as cellulose and lignin, as supported by microscopy results showing changes in nutrient distribution following humidification. The proportionality of amounts of calcium and magnesium leached and released during decomposition is likely to suggest a similarity of chemical form more than similarity of function or position of the two elements. Management of crop residues for maximising and optimising the timing of release of different nutrients will need to take into account the placement of different plant types and parts, particle sizes distribution and pre-treatment of material to efficiently manage short- and long-term soil fertility to sustain crops, particularly on degraded soils. Significant nutrient release of potassium, sulphur and magnesium from crop residues can be achieved from surface placement, with the release of potassium and sulphur managed by modifying residue particle size through appropriate harvesting, ploughing or sowing implement selection. High nutrient uptake crops and plant parts –where they can be economically viable to grow or separated by the harvesting technique – are particularly valuable as sources of nutrients and soil organic matter.