Dayspring Children’s Village, which is located about 66 km northwest of Johannesburg, has been experiencing a chronic shortage of water for over 30 years. Five out of seven boreholes that are scattered within the school property are dry and there is evidence of contamination in one of the two wells that are producing water at two depths. The presence of bluegum (Eucalyptus globulus) trees is suspected to be the principal cause for the depletion of the aquifer(s) because of their documented high rate of transpiration. The purpose of this project is to determine the complex interaction between the plants and structural elements of the various aquifers, site wells for clean water, and assist with explaining the existing contamination. This study involved parallel studies by other students. My focus was the use time-lapse DC resistivity to map preferential pathways of groundwater so that the impact of the bluegum trees could be determined. Other geophysical studies included time-domain electromagnetics, gravity and magnetics. These methods were used to map geological contacts, lithologies, geological structures, and the distribution of groundwater. Hydrogeology results from a parallel study have shown that there at least two aquifer system at Dayspring School. Time-lapse resistivity and gravity measurements were collected towards the end of rainy season and towards the end of dry season. The results show that groundwater at Dayspring School is largely structurally controlled. The impact of the bluegum trees is not obvious, but it was concluded that the trees are not the cause of apparent groundwater depletion because their root lengths do not reach the mapped geologic structures.

Severe water stress reduced the number of flowers produced by Capsicum annuum var. annuum L. Longum plants, but the timing of flower production was less affected. The use of different plant establishment techniques modified flower production. Bare rooted transplants slowed the rate of flower production in comparison with direct seeded plants or those established from cell tray transplants. Bare rooted transplants delayed the onset of water stress. The capabilities of male and female gametophytes were reduced by water stress, with the female more affected, and the interaction of male and female gametophytes was affected by moderate water stress. Moderate water stress increased the time to fruit set, but affected neither the number nor percentage of fruit set, whereas severe water stress increased the time and reduced the number and percentage. Severe water stress reduced the number and percentage of mature fruit, fruit quality indices and total fruit yield. Moderate water stress reduced the number of seeds per fruit and fruit dry weight, but total fresh weight yield was not significantly affected. The time to fruit set was negatively correlated with the quality indices of mature fruit, whereas the number of seeds was positively correlated with the same. Fertilisation determined the success of seed set, and the rapidity of fruit set. Successful fertilisation can only occur when the pollen tube arrives at a viable ovule. The processes of pollen tube growth and longevity of the ovule are the factors most critically affected by water stress in determining the yield of C. annuum Longum. / Master of Science (Hons)

Partial rootzone drying (PRD) is an irrigation management technique designed to reduce water use in grapevines without a decline in yield, thereby increasing water–use efficiency (measured as t/ML) (WUE). The principle of PRD is to keep part of the root system at a constant drying rate to produce soil-derived signals to above–ground plant organs to induce a physiological response. Major PRD effects include a reduced canopy size and greatly increased WUE with possible improvements in fruit quality. Although we have a good understanding of the hormonal physiology of PRD, little is known on the effect of PRD on partitioning of C, N and inorganic ions such as K. This thesis broadens our knowledge on the effects of PRD on grapevine field performance, growth and dry matter accumulation as well as its effects on physiology and biochemistry. In field experiments over 3 seasons, PRD reduced water use in grapevines without a significant decline in yield. PRD effects included reduced shoot growth and greatly increased WUE. Field–grown Cabernet Sauvignon, where the PRD grapevines were irrigated at half the control rate, and Shiraz where the PRD grapevines were irrigated at same rate as controls, confirmed that PRD is not simply an irrigation strategy that applies less water, rather it alters the way in which the plant responds to its environment, e.g. PRD alters the sensitivity of the stomatal response to atmospheric conditions and significantly influence enzymes that regulate nutrient accumulation and partitioning. PRD did not change the total amount of carbon and nitrogen on a whole plant basis. However, it caused a significant partitioning of carbon and nitrogen towards trunk, roots and fruit at the expense of shoot growth. This change in partitioning occurred as a result of altered activity of the enzymes controlling the assimilation of carbon and nitrogen. PRD significantly reduced nitrate reductase (NR) activity in grapevine leaves, which catalyses the first step in the assimilation of nitrate irrespective of the amount of water applied. The reduction in NR activity is correlated with the development of the PRD cycle and the associated reduction in stomatal conductance. PRD also significantly altered grapevine sucrolytic enzyme activity that regulate source:sink relationships. PRD showed transient increases in leaf sucrose phosphate synthase (SPS) activity (formation of sucrose) compared to control, but significantly reduced leaf neutral invertase (sucrose cleavage) and leaf starch content in both field and potted experiments. This may indicate an increased photosynthetic capacity and a reduction in its sink strength for sucrose in favor of organs such as fruit and roots. This hypothesis was reinforced by the fact that berries showed significantly higher levels in glucose and fructose early in the season. Berry sugar content and Brix at harvest however was unaffected. Although PRD had no significant effect on berry characteristics at harvest such as Brix and pH, it occasionally reduced per berry K+ content and increased total amino acid concentration that may lead to positive outcomes for wine quality. PRD–treated grapevine roots on the 'wet'– and 'drying'–sides differed greatly in enzyme activity and osmolality. PRD significantly increased osmolality in both wet and drying roots by increasing total osmolyte concentration that may facilitate the movement of water from wet to dry roots. The increases in osmolality were also associated with increased free polyamine production (spermidine and spermine) in PRD roots that may be related to increased root growth and density. / Thesis (Ph.D.)--School of Agriculture and Wine, 2005.

The increasing demand worldwide for Australian wine has driven the recent expansion in vineyard plantings which in turn, has increased the requirement for irrigation water in grape growing regions. Large areas of Australia's national vineyard are already irrigated with relatively poor quality water and many districts have a limited supply of water available for irrigation. Therefore, improving the efficiency of vineyard irrigation is essential for the long term sustainability of the Australian wine industry. Reducing the volume of irrigation applied to vineyards can improve water use efficiency (WUE) and reduce vine vigour. However, it can be difficult to accurately apply the required degree of water stress and this may result in a yield reduction. An irrigation technique known as partial rootzone drying (PRD) involves applying a continuous water deficit to alternate sides of the root system while ensuring the other half is well watered. This has been found to increase WUE, reduce vine vigour, improve fruit quality but not affect vine yield. Where the soil volume available for root growth is limited, so too is the resultant vine growth and yield, as access to water and nutrients is restricted. Shallow soil profiles present a major limitation to root development and grapevine vigour. In shallow soils, mounding topsoil from the vineyard mid row to form raised beds in the vine row has been found to improve vine growth and productivity. Soil mounds tend to have a higher moisture holding capacity than flat soil but the greater surface area of the mound can increase surface evaporation. Applying mulch to the mound surface has been shown to reduce evaporative soil moisture loss and conserve irrigation water. The general hypothesis tested in this experiment was that: 'Combining soil mounding, straw mulch and partial rootzone drying (PRD) irrigation will improve grapevine growth and production and reduce levels of sodium and chloride in the vine.' The experiment was established on Vitis vinifera cv. Shiraz in a mature vineyard at Padthaway, South Australia, where the soil profile consisted of a shallow loam over clay and limestone. Soils of the experimental site were classified as moderately saline because their electrical conductivity (ECse) was greater than 4 dS/m. Three main factors, irrigation method (standard or PRD), soil mounding (flat or mounded) and surface cover (bare or straw mulch) were combined into a 2X2X2 factorial experiment such that the randomised block experiment comprised three replicates of eight treatments. The irrigation treatments were control (the application of water to both sides of the vines) and PRD (the application of water to one side of the vines only at any time). In the PRD treatment the frequency of alternating the 'wet' and 'dry' sides was determined according to soil moisture measurements and was typically every 5-7 days. It was very difficult to accurately schedule the irrigation at this site to avoid applying a moisture deficit to the PRD treatment. The shallow soil profile dried very quickly following irrigation and there were problems with the accuracy of the soil moisture sensing equipment for the duration of the experiment. As a result, PRD vines experienced repeated, excessive soil moisture deficits such that vine growth and production were significantly reduced each season. Shoot length was measured weekly during the growing season, while photosynthetically- active radiation (PAR), leaf area and canopy volume were measured at full canopy. Shoot number and pruning weight were measured during dormancy. All measures of vegetative growth (with the exception of PAR) were reduced in response to PRD. The decrease in lateral shoot growth for PRD resulted in greater bunch exposure and PAR. As a direct result of the severe soil moisture deficits experienced by the PRD treatment, all components of yield were significantly reduced compared to the control treatment each season. In particular, bunch weight and berry weight were significantly lower in the PRD treatment compared to the control, which suggests a period(s) of severe soil moisture deficit was experienced. Despite the yield loss sustained by the PRD treatment, WUE was improved compared to the control treatment in the first two years of this experiment. Berry anthocyanin levels were higher for the PRD treatment than the control but this may be due to the reduction in berry size. Weekly volumetric soil moisture monitoring showed that mounded soil was wetter than flat soil each year at similar horizons. In addition, the larger soil volume of the mounded treatment enhanced vine root development. Vegetative growth was greater in the mounded treatment than the flat treatment. Mounded vines grew more shoots than non-mounded vines, although there was no effect of mounding treatment on shoot length. The difference in shoot number was significant only in year 2, possibly due to the time required for vine roots to establish in the mounds. Pruning weight and mean shoot weight were higher for the mounded treatment each year and mounded vines grew more shoots than non-mounded vines in years 1 and 2. The increase in shoot weight of mounded vines, relative to non-mounded, was most likely due to the increase in lateral shoot growth which is supported by the lower PAR values of the mounded treatment compared to the flat treatment. Each year soil mounding resulted in higher vine yields than in flat soil beds as a direct result of the increased vine capacity of mounded vines. The mounded treatment had more shoots per vine than the non-mounded treatment and thus more bunches per vine. In addition, bunch weights were higher in the mounded treatment each year, due mainly to improved fruit set and more berries per bunch. Despite the mounded treatment resulting in a denser canopy than the non-mounded treatment this did not affect fruit composition in years 1 and 2. WUE was higher for the mound treatment in years 2 and 3 only, due to the volume of irrigation water applied being reduced, yet mounded vines continued to produce higher yields than non-mounded vines. In year 3, berries from vines grown in mounded soil had significantly higher pH than berries from vines grown in flat soil beds. Mounding treatment did not consistently affect berry anthocyanin or phenolic levels. Soil moisture levels were higher in the mulch treatment than the bare treatment in all seasons. In contrast to the mounding treatment, wetter soil did not consistently lead to improved vine growth or yield. Mulched vines developed fewer roots than non-mulched vines which is likely to have limited vine access to water and nutrients. As a result, shoot growth was similar for both treatments each season. The only significant difference between treatments for pruning weight was found in year 3 and was due entirely to shoot weight. The mulched treatment had lower PAR than the bare treatment in year 3, probably the result of increased lateral shoot growth and thus increased shoot weight, although this was not significant. PAR was significantly higher for the mulch treatment, compared to the bare treatment, in year 1 only but this was not supported by significant increases in vegetative growth. The mulch treatment resulted in higher vine yield than the non-mulch treatment in years 1 and 3. This difference was significant in year 3 only when both bunch number and bunch weight were significantly higher for mulched vines. In year 1 only bunch weight was significantly higher for mulched vines. Differences between treatments occurred in year 2 for fruit composition, specifically juice TA and anthocyanin levels. The mulch treatment had significantly higher TA and a significantly lower anthocyanin concentration in berries than the non-mulch treatment in year 2. There was no evidence of increased shading in the mulched treatment relative to the bare treatment that year but the difference in anthocyanin concentration may be explained by the significantly smaller berries of the bare treatment. Analysis of samples taken regularly from the soil profile and vine rootzone showed that there was no treatment effect on soil salinity but that soil ECse increased with soil depth and time each year. Petiole samples were collected at flowering, veraison and pre-harvest and levels were deemed toxic by pre-harvest each year. The PRD treatment received approximately 60% of the salt applied to the control treatment. This did not reduce ECse but did result in lower measures of sodium and chloride in petioles and juice at harvest. Vines grown in soil mounds had access to a greater volume of soil water than the non-mounded vines. The mounded treatment had higher levels of pre-harvest petiole chloride in years 1 and 3 but this was significant only in year 3. There was no consistent trend in levels of sodium and chloride in the juice from either mound treatment, although in year 3 berry extract chloride levels were found to be significantly higher in the mounded treatment than the flat treatment. Similarly, a consistent trend in sodium and chloride levels of petioles and juice was not evident for the mulch treatment. Although, in year 3 petioles of vines grown in bare soil were found to contain significantly more petiole chloride than those which had straw mulch applied. The hypothesis that combining soil mounding, straw mulch and partial rootzone drying (PRD) irrigation will improve grapevine growth and production and reduce levels of sodium and chloride in the vine is rejected as there was not a consistent, cumulative effect of the three factors in this experiment. / Thesis (M.Ag.Sc.)--School of Agriculture and Wine, 2005.

"October 2003" / Errata inside front cover. / Bibliography: p. 289-313. / iii, 334 p. : ill., map, photo (col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / This thesis examines the influence of 'water regime', or spatial and temporal patterns in the presence of water, on the floristic composition of wetlands on the River Murray in South Australia. It explores the hypothesis that the composition of wetland vegetation is determined by components of the water regime, namely depth, duration and the rate and timing of flood and drawdown. / Thesis (Ph.D.)--University of Adelaide, Cooperative Research Centre for Freshwater Ecology and School of Earth and Environmental Sciences, Discipline of Environmental Biology, 2004

Growth, productivity and fruit quality of grapevines are closely linked to soil water availability. Withholding of water for any length of time results in slowed growth. If drought continues yield may be lost. Vines can be manipulated to stimulate early defence mechanisms by decreasing soil water availability. By using an irrigation technique, which allows for separate zones with different soil moisture status, it is possible to stimulate response mechanisms of the root system which are normally related to water stress. The difficulty of separating 'wet' and 'dry' zones was initially overcome by using split-root plants with root systems divided between two containers. Such experiments on split-root model plants resulted in the development of an irrigation technique termed partial rootzone drying (PRD). Results from irrigation experiments using PRD have shown that changes in stomatal conductance and shoot growth are some of the major components affected (Dry et al., 1996). The idea of using irrigation as a tool to manipulate stress responses in this way had its origin in the concept that root- derived abscisic acid (ABA) was important in determining stomatal conductance (Loveys, 1984). Later experiments on split-root plants have demonstrated that many effects of water stress can be explained in terms of transport of chemical signals from roots to shoots without changes in plant water status (Gowing et al., 1990). The necessary chemical signals are provided by the dry roots, and the wet roots prevent the development of deleterious water deficits. The general hypothesis tested during this study was that partial drying of the root system gives rise to a change in the supply of root-derived chemical signals which determine changes in grapevine physiology, thereby affecting fruit quality. Experiments were conducted on split-root vines (Vitis vinifera L. cvs. Cabernet Sauvignon and Chardonnay) grown in pots of different sizes, on field-grown vines which had either their root system divided by a plastic membrane (Vitis vinifera L. cv. Cabernet Sauvignon on own roots or grafted on Ramsey rootstocks) or conventional vines with a non-divided root system (Vitis vinifera L. cv. Cabernet Sauvignon, Shiraz and Riesling) with a commercial PRD irrigation design. The irrigation treatments were vines receiving water on both sides (control) and PRD-treated vines, which only received water on one side at any time. The frequency of alternation of 'wet' and 'dry' sides was determined according to soil moisture and other influences such as rainfall and temperature. In most of the experiments the irrigation was alternated from one side to the other every 10 to 15 days. Chemical signals from roots: the role of ABA and cytokinins Studies on chemical signals have concentrated on ABA and cytokinins (CK). An improved stable isotope dilution protocol, which enables analysis of ABA and CK from the same tissue sample, was developed. Analysis of cytokinins focused on zeatin (Z), zeatin riboside (ZR), zeatin glucoside (ZG) and iso pentenyl adenine (iP). Roots are relatively inaccessible, particularly in field situations. To enable easier access to roots of field-grown vines, split-root vines were planted in a trench which was refilled with a sandy soil. This created a homogenous soil substrate and did not restrict root growth while still allowing access to roots under field conditions. Analyses of root samples of field-grown vines have shown that cytokinins and ABA may originate in roots and their concentrations can be substantially altered during an irrigation cycle. Alternating soil water conditions showed that [ABA] in roots on the 'dry' side was significantly higher compared with the 'wet' side. Due to a reduction in CK on the 'dry' side of PRD-treated vines, the ratio between ABA and CK was substantially changed during an irrigation cycle. The ABA levels in root tissue and in petiole xylem sap were negatively related to stomatal conductance. This further suggests that ABA, mostly synthesized on the 'dry' side of the root system, might be responsible for a decline in stomatal conductance. Furthermore, a higher pH of petiole xylem sap was observed in PRD-treated vines which may also contribute to the regulation of stomatal conductance. Studies on stomatal patchiness showed that non-uniform stomatal aperture occurred in field-grown vines under natural environmental conditions and was more abundant under PRD conditions. The degree of stomatal opening, determined by using a water infiltration technique, correlated with measurement of stomatal conductance. Exogenous application of a synthetic cytokinin (benzyl adenine) can override the possible ABA-mediated stomatal closure resulting from PRD treatment, providing further evidence for the in vivo role of these growth regulators in the control of stomatal conductance. The effect of benzyl adenine was transient, however, requiring repeated applications to sustain the reversal. In addition, CKs may also be important in influencing grapevine growth. Following several weeks of repeated spray applications with benzyl adenine, it was found that the development of lateral shoots in PRD-treated vines was enhanced compared to PRD-treated vines sprayed with water only. This supports the idea that the reduction in lateral shoot development seen in PRD-treated vines is due to a reduced production of CKs (Dry et al., 2000a). By measuring shoot growth rate it was found that one common feature of PRD-treated vines, which were not sprayed with CK, was a reduction of lateral shoot growth. It can therefore be speculated that the reduction in lateral growth is related to a reduced delivery of cytokinins from the roots. Zeatin and zeatin riboside concentration in shoot tips and prompt buds/young lateral shoots were reduced by the PRD treatment providing further evidence in support of this hypothesis. Water movement from 'wet' to 'dry' roots Roots, being a primary sensor of soil drying, play an important role in long- and short-term responses to PRD. Using stable isotopes of water and heat-pulse sap flow sensors water movement was traced from wet to dry roots in response to PRD. The redistribution of water from roots grown in a soil of high water potential to roots growing in a soil of low water potential may be of significance with regard to the movement of chemical signals and the control of water balance of roots. Measurements of the relative water content (RWC) have shown a slower decline of RWC of the 'dry' roots of PRD vines relative to roots of vines which received no water, despite similar water content in soil surrounding those roots. The redistribution of water may help to sustain the response to PRD for longer periods possibly releasing chemical signals and to support the activity of fine roots in drying soil. Field vines, irrigated with PRD over several growing seasons, altered their root distribution relative to the control vines. PRD caused a greater concentration of fine roots to grow in deeper soil layers and this may contribute to a better water stress avoidance. The effect on root growth may be augmented by the water movement and by the large difference in ABA to cytokinin ratio, which are also known to alter root growth. PRD makes more efficient use of available water In experiments where both control and PRD-treated vines received the same amount of water many differences between the vines were demonstrated. Under conditions where water supply was adequate for both treatments, the stomatal conductance and growth of the PRD-treated vines was restricted as has been observed in many previous experiments. As total water input was reduced, however, the stomatal conductance of PRD-treated vines became greater than control vines, suggesting that the latter were experiencing a degree of water stress, whereas the PRD-treated vines were not. This may have been due to the greater depth of water penetration in the case of the PRD-treated vines, where water was applied to a smaller soil surface area. This distinction between PRD-treated and control vines, at very low water application rates, was also reflected in pruning weights and crop yields which were actually greater in PRD-treated vines. It was concluded that at low water application rates, the PRD-treated vines were more tolerant of water stress and made more efficient use of available water. Reduction in vigor opens the canopy. The initial aim of the research which led to the development of PRD was to achieve better control of undesirable, excessive shoot and foliage growth which, from a viticultural point of view, has many disadvantages. Grapevine shoot growth rate responds very sensitively to drying soil conditions. The irrigation strategy used in the PRD experiments maintained a reduction of both main shoot and lateral shoot growth. In response to PRD a decrease in shoot growth rate and leaf area was observed. Much of the reduction in canopy biomass was due to a reduced leaf area associated with lateral shoots, thus influencing the canopy structure. This was one major factor improving the light penetration inside the canopy. Control of vegetative vigour results in a better exposure of the bunch zone to light and, as a consequence, in improved grape quality. It is likely that changes in canopy density, as a result of PRD, is causing changes in fruit quality components. Anthocyanin pigments such as derivatives of delphinidin, cyanidin, petunidin and peonidin were more abundant in berries from PRD vines; by comparison the concentration of the major anthocyanin, malvidin, was reduced. When leaves were deliberately removed from more vigorous control vines, which improved bunch exposure, the differences in fruit composition were much reduced. This further supports the idea that a more open canopy, in response to PRD, improves fruit quality by affecting the canopy structure. Fruit quality consequently determines the quality, style and value of the finished wine. Wines from this study have been produced and data on wine quality from commercial wineries are also available. Sensory evaluations have demonstrated that high wine quality from PRD-treated vineyards can be achieved without any yield-depressing effects. This study has provided evidence to support the original hypothesis. The major findings were: a) Chemical signals, altered under PRD and mostly originating from roots, play an important role in the root to shoot communication in grapevines. b) The movement of water from 'wet' to 'dry' soil layers may help to sustain chemical signals as a response of grapevines to PRD and to support the activity of fine roots in drying soil. c) A reduction in vegetative growth, in particular of lateral shoots, was sustained using PRD and affected the canopy structure which in turn, due to a better light penetration into the canopy, improved the fruit quality. d) The reduction in irrigation water applied did not have a detrimental effect on grape yield and thus the efficiency of water use was improved. e) Application of relatively low irrigation rates showed that PRD-treated vines were more tolerant of water stress and made more efficient use of available water. / Thesis (Ph.D.)--Department of Horticulture, Viticulture and Oenology, 2000.

Bibliography: leaves 223-247. Effects of water availability during grain filling is examined in wheat (Triticum aestivum L.) and barley (Hordum vulgare L.) genotypes. The study tests the accumulation of dry matter (DM) and nitrogen (N) in the grain and also their remobilization from the shoot to the grain. Water stress during grain filling was found to reduce DM and N accumulation and also to increase N concentration in both wheat and barley grain.

Bibliography: p. 261-273. The general hypothesis tested in this study was that: 'partial drying of the root system of grapevines will result in reduced vegetative growth with beneficial effects for fruit yield, fruit composition and water-use efficiency'. Previously published work has suggested that partial drying of the root system may give rise to non-dydraulic root-derived signals capable of changing leaf development and transpiration.

Includes bibliographical references (p. B1-B24)

No description available.