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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Determinación de la actividad antibacteriana “in vitro” del aceite esencial de Luma chequen (Molina) A. Gray “arrayán” frente a Streptococcus mutans

Flores Romero, Jessica January 2014 (has links)
El objetivo del presente estudio fue determinar la actividad antibacteriana del aceite esencial de Luma chequen (Molina) A. Gray “arrayan” frente a Streptococcus mutans. Se empleó el método de disco-difusión en agar. La cepa fue reactivada en placa de Tripticasa Soya Agar (TSA), incubada a 37 ºC por 24 horas en microaerofilia. Se tomaron 5 colonias y se transfirieron a un tubo de ensayo con 5 mL de caldo Müller-Hinton, incubada a 37 ºC por 6 horas en microaerofilia hasta obtener una turbidez equivalente al 0.5 de la escala de Mc Farland. El sembrado se realizó en 14 placas con agar Müller-Hinton mediante la técnica de difusión, utilizando el aceite esencial en concentraciones de 10, 50 y 100%, y se procedió a la incubación en microaerofilia a 37 ºC por 24 horas. El análisis estadístico se realizó con el programa STATA Versión 12. Las concentraciones al 10, 50 y 100% presentaron un halo de inhibición promedio de 6.28, 7.88 y 8.66 mm respectivamente, la diferencia de promedios entre estas tres concentraciones mostró diferencia estadísticamente significativa (P<0.05). Se concluye que las tres concentraciones del aceite esencial de Luma chequen (Molina) A. Gray “arrayan”, presentan actividad antibacteriana frente a Streptococcus mutans. / --- The aim of this study was to determine the antibacterial activity of the essential oil of Luma chequen (Molina) A. Gray "myrtle" against Streptococcus mutans. The disk-diffusion method in agar was used. The strain was reactivated in Trypticase Soy agar plate (TSA), incubated at 37 °C for 24 hours in microaerophilic. 5 colonies were picked and transferred to a test tube with 5 mL of Mueller-Hinton broth, incubated at 37 °C for 6 hours under microaerophilic conditions until a turbidity equivalent to 0.5 Mc Farland scale. Seeding was performed on 14 plates with Mueller-Hinton agar by diffusion technique, using the essential oil in concentrations of 10, 50 and 100%, and the incubation proceeded in microaerophilic at 37 ºC for 24 hours. Statistical analysis was performed using STATA Version 12 program. Concentrations of 10, 50 and 100 % had a halo of inhibition average of 6.28, 7.88 and 8.66 mm respectively, the mean difference between these three concentrations showed statistically significant difference (P<0.05). We conclude that the three concentrations of the essential oil of Luma chequen (Molina) A. Gray "myrtle", exhibit antibacterial activity against Streptococcus mutans. Keywords: Streptococcus mutans, myrtle, antibacterial activity. / Tesis

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