This study sought opportunities to mitigate the pressures of land transformation and alien invader plants on commercial farm land in the thicket biome in the lower reaches of the Fish Kowie Corridor. It had two aims. Firstly, to determine the role incentives could play in mitigating these pressures. Secondly, to determine the characteristics of an incentive programme that would most effectively achieve this. In order to do this, an understanding of landowner activities, needs, opinions and barriers to behaviour; the nature of the pressures on thicket and the nature of the required behaviour to reduce these pressures; and current and past institutional arrangements needed to be achieved. This was done predominantly through a current literature review and personal interviews with landowners and key informants. These findings were used to make recommendations for an effective incentive programme. Landowners showed a preference towards tangible incentives, in particular management assistance, financial compensation and law enforcement. They indicated an aversion to an incentive programme implemented by a government agency, particularly district and provincial government. Rather, landowners showed a propensity towards a nongovernment organisation (NGO) or a farmers group implementing an incentive programme. It was recommended that the two major pressures, namely land transformation and alien invader plants, required different interventions by different agencies in order to be mitigated. The pressure of land transformation required a stewardship model response, with the primary drive being a non-contractual environmental extension service to landowners. The extension service should focus on promoting pro-conservation practises, raising awareness and disseminating information. It should also build a relationship of trust between landowners and the implementing agency. The pressure of alien invader plants would be most effectively addressed through the Working for Water programme. Tangible incentives must be provided to the landowner to induce the costly exercise of alien invader plant control. In particular, the high cost of labour must be addressed. The regulatory incentive of applying laws requiring landowners to control alien invader plants on their land should also be enforced.

Over the past 25 years, there has been a resurgence of worldwide scientific research in the fields of ethnopharmacology. The Western world has acknowledged the continued use of traditional medicines by the majority of third world countries, and the need for novel drug development. Hence, much of the pharmaceutical research in recent years has focused on the ethnobotanical approach to drug discovery (Light et al., 2005). In South Africa, as in most developing parts of the world, traditional herbal medicine still forms the backbone of rural healthcare. The government health services in South Africa provide only western medical care although the majority of the population consult traditional healers for some or all of their healthcare needs (McGaw et al., 2005). Medicinal plants like Harpephyllum caffrum are used as blood purifiers or emetics (Watt and Breyer-Brandwijk, 1962), and also for treating acne and eczema. The antimicrobial activity of this plant can be used to treat septicaemia, which is ranked the sixth leading cause of death among neonates and the eighth leading cause of death for infants through the first year of life (Heron, 2007). In this study, the plants investigated for antimicrobial activity were Harpephyllum caffrum, Hermannia cuneifolia, Chironia baccifera, Rhigozum obovatum, Felicia muricata and Pentzia incana. These plants were tested against ATTC (American Type Culture Collection) strains and microorganisms isolated from clinical isolates of patients suffering from septicaemia. The assay methods used included the agar diffusion method using the Mast multipoint inoculator, the microtitre dilution method were used to determine the minimum inhibitory concentration, thin layer chromatography fingerprints accompanied by bioautographic assay were used to detect the inhibition of bacterial growth by active compounds separated from plant extracts and the Ames test was required to assess the possibility of bacterial mutagenesis upon the exposure to plant extracts which can lead to carcinogenicity. In agar diffusion method, extracts of Harpephyllum caffrum inhibited nine strains of Candida albicans, three species of Acinetobacter and four strains of E.faecalis. Extracts of Hermannia cuneifolia inhibited four strains of B.cereus and three strains of Staphylococcus aureus. Extracts of Chironia baccifera inhibited one strain of Acinetobacter and five strains of E.faecalis. Extracts of plants Rhigozum obovatum, Felicia muricata, and Pentzia incana showed no antimicrobial activity. In the microtitre dilution method used to determine the minimum inhibitory concentration (MIC), the results were different from the agar diffusion method. More activity was observed. Extracts of Harpephyllum caffrum inhibited three strains of E.coli, six strains of S.aureus, three species of Acinetobacter and one strain of Klebsiella pneumonia. Extracts of Hermannia cuneifolia inhibited four strains of B.cereus, three strains of S.aureus, two strains of K.oxytoca and one species of Acinetobacter. Extracts of Chironia baccifera inhibited three strains of S.aureus, one strain of MRSA, one species of Acinetobacter and one strain of S.haemolyticus. The MIC values ranged from 0.049 to 6.25mg/ml. Using the thin layer chromatography fingerprints, bioautography showed the presence of various inhibitory chemical compounds. Methanol and acetone extracts of Harpephyllum caffrum, separated very well and showed various inhibition zones on exposure to Candida albicans, Enterococcus faecalis and Staphylococcus aureus. The different inhibition zones were recorded as Rf In the Ames test (Maron and Ames, 1983) the methanol and acetone extracts of Harpephyllum caffrum and Hermannia cuneifolia were negative which means they were devoid of any mutagenic properties. Methanol extracts of Harpephyllum caffrum showed similar results in the Ames assay as reported by Verschaeve and Van Staden (2008). values ranging from 0.25 to 0.95. The zones indicate the different inhibiting chemical compounds present in the plant. Petroleum ether, ethyl acetate, chloroform and formic acid were the solvents used in the assay in the ratio 8:7:5:1, respectively. Establishing the antimicrobial activity of these plants contribute to the systematic scientific investigation of indigenous South African medicinal plants.

Alcohol abuse is a very common practice (just like in many other parts of the world) in Nkonkobe Municipality, Eastern Cape Province, South Africa. This is associated with liver disease. An ethnobotanical survey of plants used for the treatment of alcohol-induced liver damage in Nkonkobe Municipality was conducted. During the survey and also from information gathered in the literature, Pelargonium reniforme Curtis, was prominently mentioned, among other plants, as the species used generally for the treatment of alcohol-induced liver damage. This project was designed to evaluate the effects of the plant on alcohol-induced liver damage, including its antioxidant and antimicrobial properties. It also involves safety evaluation studies to determine if the plant is safe for consumption. Studies using rats of the Wistar strain were carried out to determine the protective and curative effects of P. reniforme on alcohol-induced liver damage. Results obtained showed that the plant extract can protect the liver cells as well as enhance recovery from tissue damage. The plant also showed good antimicrobial and antioxidant activity and this further validates its use in the treatment of liver diseases. Safety evaluation studies of the extract were carried out by investigating the effects of the oral administration on some haematological and biochemical parameters in male Wistar rats. The results obtained from the study suggest that the plant extract is not toxic at the doses used and is therefore safe for medicinal uses. The results of the various bioassays carried out in this project have justified the traditional uses of P. reniforme for the treatment of alcohol-induced liver damage.

The invasion of Acacia mearnsii in the Kouga catchment, Eastern Cape, South Africa, has various negative impacts on the ecosystem. These impacts include: reduced species richness, increased water use, increased nutrients and increased N cycling rates. The native shrubby fynbos vegetation has adapted to the acidic nutrient poor soils and Mediterranean climate of the Kouga Mountains. Fynbos, however, is currently being out competed by the much taller Acacia mearnsii trees, due to their competitive nature and ability to fix nitrogen, thereby enriching the soil. The invaded sections of the valley bottoms and lower hill slopes are characterised by an almost complete monoculture of Acacia mearnsii, with very few fynbos species still present. The Department of Water and Environmental Affairs sponsored Working for Water programme started clearing Acacia mearnsii in 1996 in the Kouga Mountains. Cleared sites have remained bare for long periods, indicating that soil properties are not favourable for indigenous propagule re-establishment. The aim of this research was to assess how A. mearnsii invasion and clearing affect fynbos recovery through its impact on soils. This was done by characterising vegetation and soil properties on fynbos, infested and cleared slopes. Vegetation cover for various growth forms was determined and a species list was compiled for each plot. The slope angle, surface hardness, litter cover, bare ground cover and soil depth were measured in the field, whereas water repellency, particle size and the chemical composition were measured in the laboratory. Furthermore, the plant establishment capacity of soils from fynbos, infested and cleared slopes was calculated. This was done by germinating fynbos seeds and growing fynbos plants in soils from the various slopes. The effect that invasion and clearing has on soil erosion was quantified using erosion plots on fynbos, infested and cleared slopes. The invasion and clearing of Acacia mearnsii led to an increase in soil nutrients, especially nitrogen, phosphorus, potassium, carbon and manganese. Furthermore, soils became more acidic, with increased water repellency and reduced surface hardness. The vegetation changed to a tree-dominated structure, replacing the native species. Native plant germination was relatively unaffected by invasion and clearing, with an increase in germination just after clearing. Plant growth of a native grass, Themeda triandra, and herb, Helichrysum umbraculigerum, has increased on soils from cleared slopes. This study showed that soil movement increased on slopes which are invaded and cleared of Acacia mearnsii, with erosion rates doubling on invaded slopes

Oldenburgia grandis is a rare, long-lived woody paleoendemic of the Fynbos Biome of South Africa. Confined to quartzite outcrops, it has a small geographic range and narrow habitat specificity. O. grandis responds to its fire-prone environment by resprouting. Elasticity analysis of O. grandis reveals that growth and fecundity were traded off for persistence of adult, mature and sapling stages. Morphological adaptations such as a corky fire-resistant bark and the ability to resprout after fire are traits that O. grandis have evolved to persist in a frequently disturbed environment. Population growth rate for sites undisturbed by fire for a number of years (l = 1.01) and sites at various stages of recovery after fire (l = 1.00) were very similar. The highest variation in transition probabilities for all sites was seen in the persistence of the seedling stage and growth from seedling to sapling. Observed population structure and stable stage distribution determined by the matrix model show that sites recently undisturbed by fire had high abundances of the adult and sapling stages. A peak in sapling stages was seen for the stable stage distribution where similar peak in sapling numbers were seen for population structures of sites at various stages of recoveryafter fire. Favourable environmental conditions for the persistence of O. grandis populations include no fire with transition probabilities between the observed minimum and maximum and fire frequency at a 10 year interval where seedling protection from the fire is high and adult and mature mortalities during the fire are low. Stochastic environmental events that could put populations (particularly small populations) at an increased risk of extinction include high to moderate fire intensities where seedling protection from the fire is low and adult and mature mortalities are high as a result of the fire.

This study looks at the impact of elephant feeding on the Xeric Succulent Thicket component of Eastern Cape Subtropical Thicket (ECST) in Addo Elephant National Park (AENP). Observations of elephant feeding were carried out and vegetation transects were surveyed for impact of elephant feeding. The results indicated that the Nyati elephants spent the majority of their time grazing (nearly 90%), particularly the cow-young herds, and especially when the herd gathered in larger numbers. Browsing events were concentrated on Acacia karroo (81%) and there was no significant difference between the sexes in their preference for this species. Despite being subjected to most of the browsing, the majority of A. karroo trees were undamaged and the effect of elephants was generally light. It appears unlikely that, three years after re-introduction to Nyati, the elephants have had an effect on community structure of the vegetation. Surveys were conducted on stands of the alien invasive weed prickly pear Opuntia ficus-indica, and it was recorded that elephants in Nyati have had a dramatic effect on prickly pear, utilising all adult plants assessed and destroying 70% of them. This level of destruction in such a short period of time suggests that prickly pear is a highly favoured species. The results from the present study suggest that elephants can play a role in the control of prickly pear. Results are discussed in terms of elephants as both megaherbivores and keystone species, and as agents of intermediate disturbance.

The Kouga Riparian Rehabilitation Project (KRRP) is seen as a pilot rehabilitation project in the Kouga region that is heavily invaded with Acacia mearnsii along the riparian zones of many mountain streams. Clearing of these black wattles and re-planting of indigenous vegetation are imperative to rehabilitation efforts. In this context, two invaded catchments were identified - the Baviaans and the Heuningnes. The aim of this research is to characterise the effects that the woody alien invasive Acacia mearnsii has had on the river channel morphology of the Baviaans and Heuningnes Rivers. A desktop and initial field analysis of the relevant study area catchments was completed. This was followed by a comparison of the channel morphology of the various study channel reaches using fixed channel transects. Ecological resource quality objectives (RQOs) for river rehabilitation from a fluvial geomorphological viewpoint were then established. A long-term monitoring protocol to assess whether or not these RQOs will be achieved was recommended. Follow-up channel transects were measured post wattle clearance in the Baviaans and short-term (<2yrs) changes in channel form were described. Differences in terms of the effect of Acacia mearnsii on channel form were then interpreted by direct comparison and through statistical analysis. Results indicate a number of significant differences between those channels impacted by black wattle infestation and those channels seen as unimpacted and natural. Short-term changes (<2yrs) that occurred within the study period post Baviaans wattle clearance were shown to be minimal for channel form as well as for bed material. The lack of any clear relationship or explanation between channel form and other channel controls suggests vegetation as the primary control. Vegetation, specifically the invasive alien vegetation, is the key controlling variable acting on channel form in the two study catchments.

Water is an indispensible and yet a difficult resource to be renewed, thus water scarcity has become one of the major challenges faced worldwide, with the Southern regions of Africa being the most impacted and affected, especially the Eastern Cape Province of South Africa where rural communities depend on receiving waterbodies that are often negatively impacted by wastewater final effluents. This present study was conducted between August and December 2010 to assess the physicochemical and microbial qualities of the final effluents of peri-urban and rural communities based wastewater treatment plants in the Eastern Cape Province. The physicochemical parameters were determined on site and in the laboratory, while bacteriological qualities were determined using culture based techniques. The virological qualities were determined by molecular methods using reverse transcriptase polymerase chain reaction for the target RNA virus and the conventional polymerase chain reaction for the target DNA virus. For both wastewater treatment plants, the physicochemical parameters ranged as follows: chemical oxygen demand (5.95-45 mg/L); total dissolved solids (114.5-187.0 mg/L); salinity (0.12-0.20 psu); temperature (14.2-25.7oC); pH (6.0-7.6); nitrate and nitrites (1.55-6.7 mg/L and 0.023-1.15 mg/L respectively); biological oxygen demand (3.5-7.8 mg/L); turbidity (1.49-6.98 NTU); and chlorine residual (0-2.97 mg/L). Feacal indicator bacteria counts ranged as follows: feacal coliforms (0-1.25×104 cfu/100 ml); total coliforms (0-3.95×104 cfu/100 ml); and enterococci (0-5.0×103 cfu/100 ml). xviii Seventy five percent of the rural community based plant and 80 percent of the peri-urban community based plant were positive for coxsackie A virus, while hepatitis A virus was detected in all the rural community based plant 80 percent of the peri-urban community based plant. This study suggests the need for intervention by appropriate regulatory agencies to ensure regular monitoring of the qualities of final effluents of wastewater treatment facilities in the Eastern Cape Province and ensure compliance to established guidelines.

Worldwide, water reuse is promoted as an alternative for water scarcity, however, wastewater effluents have been reported as possible contaminants to surface water. The failure of some wastewater treatment processes to completely remove organic matter and some pathogenic microorganisms allows them to initiate infections. This manifests more in communities where surface water is used directly for drinking. To assess water quality, bacteria alone cannot be used as it may be absent in virus-contaminated water. This study was carried out to assess the quality of two wastewater treatment plant effluents from the Eastern Cape Province of South Africa. Physicochemical parameters and microbiological parameters like faecal coliforms, adenovirus, rotavirus, hepatitis A virus, norovirus and enterovirus were evaluated over a projected period of one year. Physicochemical parameters were measured on site using multiparameters, faecal coliforms enumerated using culture-based methods and viruses are detected using both conventional and real-time PCR. Physicochemical parameters like electrical conductivity, turbidity, free chlorine and phosphates were incompliant with the standards set by the Department of Water affairs for effluents to be discharged. Faecal coliform counts were nil for one plant (WWTP-R) where they correlated inversely (P < 0.01) with the high free chlorine. For WWTP-K, faecal coliforms were detected in 27% of samples in the range of 9.9 × 101 to 6.4× 104 CFU/100ml. From the five viruses assessed, three viruses were detected with Rotavirus being the most abundant (0-2034176 genome copies/L) followed by Adenovirus (0–275 genome copies/L) then Hepatitis A virus (0–71 genome copies/L) in the WWTP-K while none of the viruses was detected in WWTP-R. Species B, species C and Adv41 serotypes were detected from the May 2013 and June 2013 samples where almost all parameters were incompliant in the plant. The detection of these viruses in supposedly treated effluents is suggestive of these being the sources of contamination to surface water and therefore renders surface waters unsafe for direct use and to aquatic life. Although real-time PCR is more sensitive and reliable in detection of viruses, use of cell-culture techniques in this study would have been more efficient in confirming the infectivity of the viruses detected, hence the recommendation of these techniques in future projects of this nature.

South Africa, many plants which are used in traditional medicines are collected from wild populations. The high demand for trade and use of these medicinal plants place an enormous pressure on their natural populations, especially because they are indiscriminately harvested. The most affected of these plant species are those harvested from their underground parts, among which is Gunnera perpensa L. Gunnera perpensa is of considerable ethnobotanical interest in traditional medicine because of its wide usage. The rhizomes are widely used and indiscriminately collected in large quantities from the wild to meet the ever increasing demand in traditional medicine markets. As a result, this valuable medicinal plant species is being endangered. According to the Red List of South African Plants, the conservation status of G. perpensa has been listed as ‘declining’. The ethnobotanical survey conducted as part of this research confirms the plant species as threatened. It is, therefore, important to develop propagation and conservation strategies for this medicinal plant. Clonal propagation of G. perpensa was conducted using varying lengths of the rhizome (1, 2, 3, 4 and 5 cm) segments as propagules. While regeneration was possible with all the rhizome lengths, most of the growth parameters were significantly higher in the 5 cm rhizomes than the other rhizome segments. The appropriate planting depth for the rhizomes was also determined and 4 or 5 cm planting depths were found appropriate. No significant difference was observed in the growth parameters amongst the planting depths; nevertheless, 4 cm depth gave higher growth and yield. The results of this study show that regenerating G. perpensa.