Made available in DSpace on 2014-12-02T11:16:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-12-06Bitstream added on 2014-12-02T11:21:40Z : No. of bitstreams: 1 000791255.pdf: 1126361 bytes, checksum: e2fdc0b9ded8a59775319f1afd5a8687 (MD5) / A máxima qualidade das sementes de soja é expressa na maturidade fisiológica, mas, a colheita nesse estádio fenológico não é recomendada, devido ao elevado teor de água nas sementes e o excesso de folhas e hastes verdes nas plantas. Com o intuito de antecipar a colheita, a prática da dessecação química, com o herbicida glyphosate, vem sendo estudada em soja e requer alguns procedimentos, visando a preservação da qualidade fisiológica das sementes no campo. Desse modo, o trabalho teve como objetivo avaliar a qualidade fisiológica de sementes de soja de cultivares convencionais e transgênicas, oriundas de plantas dessecadas com herbicida glyphosate, em diversas épocas de dessecação. A dessecação química com o herbicida glyphosate (2,0 L.ha-1) foi realizada quando os estádios fenológicos R6.0, R7.1, R7.2 e R7.3 das plantas das cultivares BRS 133, Conquista, BRS 243RR e BRS 246RR foram atingidos. As determinações e testes para avaliação da qualidade das sementes foram: teor de água, massa de 100 sementes, germinação, primeira contagem de germinação, envelhecimento acelerado, emergência de plântulas no campo, índice de velocidade de emergência de plântulas no campo, condutividade elétrica, comprimento de plântulas e massa de matéria seca de plântulas. O delineamento experimental adotado foi o de blocos casualizados, dispostos em esquema de parcelas subdivididas, com quatro repetições, totalizando 20 tratamentos. As parcelas constaram de quatro cultivares (BRS 133, Conquista, BRS 243RR e BRS 246RR) e as subparcelas corresponderam a quatro épocas de dessecação, que corresponderam a quatro estádios da cultura (R6.0, R7.1, R7.2 e R7.3) e uma testemunha para cada cultivar (sem dessecação). Os tratamentos foram dispostos em esquema fatorial com 4 cultivares x 4 épocas de dessecação, mais as testemunhas e as médias foram comparadas pelo teste de ... / The maximum quality of soybean seeds is expressed in physiological maturity, but, harvest is not recommended in this phase, due to the high water content in seeds and excess leaves and green stems on the plants. With the aim of anticipating the harvest, the practice of chemical desiccation, with the herbicide glyphosate, has been studied in soybean and requires some procedures, aiming at the preservation of the physiological quality of seeds in the field. In this way, the work aimed to evaluate the physiological quality of soybean seeds of conventional and transgenic cultivars from desiccated plants with herbicide glyphosate, in various periods of desiccation. Chemical desiccation with the herbicide glyphosate (2,0L.ha-1) was performed when the phenological stages R6.0, R7.1, R7.2 and R7.3 plants of cultivars BRS 133, Conquista, BRS 243RR and BRS 246RR were achieved. Tests for evaluation of the quality of the seeds were: water content, mass of 100 seeds, germination, first count of germination, accelerated aging, emergence of seedlings in the field, speed index of seedling emergence in the field, electrical conductivity, seedling length and mass of the dry matter of seedlings. The experimental design was randomized block, arranged in plots scheme subdivided, with four repetitions, totaling 20 treatments. The parcels consisted of four cultivars (BRS 133, Conquista, BRS 243RR and BRS 246RR) and the subplots corresponded to four periods of desiccation, which corresponded to four stages of culture (R6.0, R7.1, R7.2 and R7.3) and a witness to every cultivar (without desiccation). Treatments were arranged in factorial scheme 4 cultivars x 4 times of desiccation, more witnesses and the averages were compared by t test the probability 5. The application of glyphosate in phenological stages R6.0 and R7.1 of soybean plants, conventional cultivars BRS 133 and Conquista, negatively influences the physiological ...

Orientador: Caio Antonio Carbonari / Coorientador: Anderson Luis Cavenaghi / Banca: Elza Alves Correa / Resumo: As cultivares de algodão Liberty Link ® são aquelas que resistem à aplicação do amônio glufosinate a uma dose que normalmente seria recomendada para os demais biótipos da espécie. Estas cultivares de algodão são constituídas geneticamente com um gene que codifica a produção da enzima fosfinotric ina acetil transferase (PAT), responsável pela acetilação do amônio glufosinate, inativando - o na planta. A cultivar de algodão FiberMax ® 975 WideStrike por também apresentar em seu DNA o gene PAT apresentaria o comportamento de seu metabolismo intermediário às cultivares convencional e Liberty Link ® . O objetivo do trabalho foi avaliar as alterações metabólicas e a intoxicação das plantas de algodão Wide Strike ® comparadas a plantas Liberty Link ® e convencionais, quando submetidos ao herbicida amônio glufosinate. Os es tudos foram conduzidos em casa de vegetação n a Faculdade de Ciências Agronômicas - FCA / UNESP - Campus de Botucatu (SP). Dois experimentos foram instalad os simultaneamente utilizand o cultivares as cultivares transgênicas e convencional , sendo o primeiro para avaliar a intoxicação e alterações na fotossíntese e o segundo para quantificar compostos relacionados a rota de ação do herbicida amônio glufosinate . Foram semeadas 5 sementes p or vaso de três cultivares de algodão FM 993 (convencional), FM 975WS ® (transgênica) e IMACD 6001LL ® (transgênica) e, aos 15 dias após a emergência (DAE) foi realizado o desbaste das plantas, mantendo duas plantas por vaso. Quando as plantas apresentaram 2 folhas verdadeiras (25 dias após emergência) realizou - se a primeira aplicação dos ... / Abstract: The Liberty Link cotton cultivars are those that resist a dose of ammonium glufosinate application that normally would be recommended for other biotypes species .These cotton plants are constitute genetically with gene that encodes the production of enzyme phosphinothricin acetyl transferase (PAT) responsible for the acetylation of glufosinate ammonium, inactivating it in the plant. The cotton cultivar FiberMax® 975 WideStrike has present in i ts DNA the gene PAT and present intermediate metabolism to conve ntional cultivars and Liberty Link ®. The purpose of the study was to evaluate the metabolic changes and the intoxication of WideStrike®cotton plants compared with Liberty Link ® and conventional plants, when treated to ammonium glufosinate herbicide. The studies were carried out in a greenhouse at the Faculty of Agricultural Sciences, "Júlio de MesquitaFilho" State University - Botucatu Campus (SP). Were conducted two experiments simultaneously using the transgenic and conventional cultivars, the first one to evaluate the toxicity and changes in photosynthesis and the secondto quantify compounds related to the herbicide ammonium glufosinate action route. Five seeds were sown of each cultivar per pot of the three cotton cultivars FM 993 (non - genetically modi fied), FM 975WS® (genetically modified) and IMACD 6001LL® (genetically modified) and at 15 days after emergence (DAE) was performed thinning of the plants, keeping two plants per pot. When the plants had two true leaves (25 days after emergence) held the ... / Mestre

MSc. (Geography) / Growing concerns with regards to the environmental and economic impacts related to the application of herbicides to control the spread and abundance of weeds in agricultural crops have created a need for the development of novel agricultural management systems that are less dependent on herbicide usage and tillage. Such concerns have given rise to the need for the variable spatial treatment of croplands aimed at the minimization of requirements for the application of herbicides and the subsequent minimization of excess materials released into the surrounding environment. Remote sensing provides an opportunity for the fast and cost-effective delineation of weed patches in croplands over large areas where traditional scouting techniques would be impractical. The differences in spectral reflectance from different plants at certain wavelengths due to species specific variations in biochemical and physical characteristics is what lays the basis for the distinction of vegetation species within remotely sensed images and ultimately the potential detection of weed-species in croplands. This study investigates the potential spectral characterisation of maize and commonly occurring weed-species by (1) making use of reflectance spectra collected at leaf-level to identify statistically significant differences in reflectance between individual species throughout the visible (VIS), Near-Infrared (NIR) and Shortwave-Infrared (SWIR) regions of the electromagnetic spectrum, determining the potential of the Red-Edge Position (REP) and slope for this particular application and testing the accuracy at which reflectance spectra may be classified according to vegetation species based on spectral reflectance at specific wavebands and REP as input predictor variables, (2) testing the potential effect of mixed spectral responses and soil-background interference through the analysis of reflectance spectra collected at canopy-level, and (3) determining the potential effect of the spectral generalisation associated with multispectral reflectance through the analysis of spectral responses resampled to the spectral band designations of representative high spatial resolution multispectral sensors. The results showed that maize may be spectrally distinguished from all of the weed-species included in the analysis based on leaf-level hyperspectral reflectance throughout the Visible-to-Near-Infrared (V-NIR) and SWIR-regions of the electromagnetic spectrum, however, the unique characterisation of weed-species is not possible for all species and where it is possible, it is highly wavelength-specific and would require high spectral resolution hyperspectral data. The wavelengths most suitable for the spectral characterisation of maize-crops and weed species in the study area were identified as: 432.1nm, 528.2nm, 700.7nm, 719.4nm, 1335.1nm, 1508.1nm, 2075.8nm, 2164.5nm and 2342.2nm. The output predictor model was able to classify reflectance spectra associated with maize crops and weeds in the study area at an overall accuracy of 89.7 per cent and it was shown that the inclusion of the REP as predictor variable did not improve the overall accuracy of the classification, however, may be used to improve the classification accuracies of certain species...

The interactions of paraquat (methylviologen) and diphenylether herbicides with the Mehler reaction as investigated. Sera from two different rabbits (RS1 & RS2) were examined for their patterns of inhibition of the photosynthetic electron transport (PET) system. Serum from RS2 was greatly hemolyzed. Fifty ul of RS1 serum were required for 100% inhibition of a H₂O → methylviologen(MV)/O₂ reaction, whereas only 10 µl of a 1:10 dilution of RS2 were needed for 100% inhibition. The γ-globulin fraction from purified rabbit serum (RS1) did not inhibit PET, indicating that the antibody fraction of the rabbit serum does not contain the inhibitor. It appears that the inhibitor is from the hemolyzed red blood cells. Rabbit sera, added to chloroplast preparations prior illumination, caused no inhibition of a H₂O → MV/O₂ reaction while addition of rabbit sera during illumination inhibited the H₂O → MV/O₂ reaction within 1-3 s. Various Hill reactions were used to determine the site of inhibition. Rabbit sera inhibited photosystem I (PSI) Hill reactions, but did not inhibit a photosystem II (PSI II) Hill reaction indicating that inhibition is on the reducing side of PSI. It would be expected that a H₂O → Ferredoxin (Fd)/NADP Hill reaction should also be blocked. Surprisingly, rabbit sera did not inhibit this reaction. These results were interpreted as supportive evidence for parallel (branched) electron transport on the reducing side of PSI. Six pyridyl derivatives {benzylviologen, 2-anilinopyridine, 1,2-bis(4-pyridyl)ethane, 1,2-bis(4-pyridyl)ethylene, 2-benzoylpyridine, and 2-benzylaminopyridine} and five heme-iron derivatives {hemoglobin, hemin, hematin, ferritin, and ferrocene} were screened for their potential to counteract paraquat toxicity on pea (Pisum sativum L. cv. Little Marvel) isolated chloroplasts. H₂O → MV/O₂ and H₂O → Fd/NADP+ were the two Hill reactions assayed with these compounds. Antagonists of paraquat toxicity should inhibit the first Hill reaction but not the latter. None of the pyridyl derivatives examined inhibited the reaction H₂O → MV/O₂. Ferritin and ferrocene were also ineffective as inhibitors of this reaction. Hemoglobin inhibited the reaction H₂O → MV/O₂ without inhibiting the reaction H₂O → Fd/NADP+, providing protection to pea chloroplasts against paraquat. Hemin and hematin inhibited both Hill reactions examined. Hemin and hematin also inhibited H₂O → diaminodurene (ox) and durohydroquinone → MV/O₂ Hill reactions but not the dichlorophenylindolphenol(red) → MV/O₂ and diaminodurene(red) → MV/O₂ Hill reactions. These results indicate that hemin and hematin are inhibiting photosynthetic electron transport in the plastoquinone pool region. Potential involvement of hydroxyl and alkoxyl radicals in the peroxidative action of the p-nitro diphenyl ether herbicides acifluorfen was evaluated under laboratory conditions. Methional was added to illuminated pea thylakoids and its oxidation to ethylene was used as an indicator of hydroxyl and alkoxyl radical synthesis. Oxyfluorfenstimulation of the rate of methional oxidation was dependent on light, photosynthetic electron transport and hydrogen peroxide since it was not observed under dark conditions or in the presence of DCMU and catalase. Addition of FeEDTA, a catalyst of the Fenton reaction, stimulated the oxyfluorfen-induced enhancement of methional oxidation six-fold suggesting that hydroxyl radicals are synthesized through a Fenton reaction. Acifluorfen, nitrofen and nitrofluorfen inhibited the rate of methional oxidation whereas, acifluorfen-methyl had no effect on the rate of methional oxidation even at high concentrations (1 mM). Nitrofluorfen at 1 mM was the only p-nitro diphenyl ether herbicide tested which inhibited photosynthetic electron transport of pea thylakoids. In experiments with pea leaf discs, acifluorfen at low concentrations stimulated the rate of methional oxidation, while acifluorfen-methyl, nitrofen and nitrofluorfen had no effect. These data indicate that hydroxyl and alkoxyl radicals could be involved in the mechanism of cellular damage caused by oxyfluorfen, but they are not important for the activity of the diphenyl ether herbicides acifluorfen, acifluorfen-methyl, nitrofen, and nitrofluorfen. Diethyldithiocarbamate (DEDTC) does not accept electrons from the photosynthetic electron transport (PET), but can donate electrons to a photosystem I (PSI) Mehler reaction in the presence of the following PET inhibitors: diuron, dibromothymoquinone, and bathophenanthroline. It cannot photoreduce PSI in the presence of cyanide, a PET inhibitor. These data indicate that the site of electron donation is after the plastoquinone pool. Ascorbate is not required for the ability of DEDTC to donate electrons to PSI. There is no photoreductant activity by DEDTC in a ferredoxin/NADP Hill reaction. Superoxide dismutase inhibits DEDTC/diuron or bathophenanthroline → MV/O₂ Mehler reaction. Catalase does not restore the consumed O₂ from a DEDTC/diuron → MV/O₂ Mehler reaction, indicating O₂- has not been dissmutating into H₂O₂. These results indicate that superoxide is required for DEDTC ability to donate electrons, therefore DEDTC is limited only to Mehler-type reactions. / Ph. D.

Field and laboratory studies were conducted to determine the ecological and physiological responses of phytoplankton to environmentally significant concentrations of atrazine, diuron, and 2,4-D. Field results would indicate that all three herbicides have a significant effect on species diversity and species dominance. Atrazine and diuron were particularly effective in decreasing diversity in concentrations of 10 ppb to 1.0 ppm. While total biomass as measured by ATP decreased significantly for both atrazine and diuron, total chlorophyll levels increased significantly in 0.1 mg/l atrazine. Decreases in chlorophyll were noted for all other concentrations tested in atrazine and diuron. Blue-green algae Gloeocapsa sp. and Synecchoccus sp. were the predominant genera in the 0.1 mg/1 atrazine. 2,4-D tests also resulted in significant increases in chlorophyll over control replicates. Additionally, biomass as measured by ATP indicated significant increases over control values in 10 mg/1 2,4-D suggesting that 2,4-D may have stimulated the growth of algae or that select algae within the test were able to take a competitive advantage in the presence of 2,4-D. This result was not consistent however. Laboratory tests using continuous cultures of Chlorella sorokiniana indicated that both atrazine and diuron were effective toxicants in low concentrations. Diuron was an effective growth inhibitor in a range of concentration from 0.0014 to 0.01 mg/l. Atrazine was an effective growth inhibitor from 0.014 to 0.1 mg/l. 2,4-D stimulated the growth of continuously cultured Chlorella sorokiniana in a range of concentration from 8.6 to 20 mg/l as measured by cell counts, total organic carbon, in vivo chlorophyll fluorescence, and ATP. The data indicated that the effect was not due to the utilization of 2,4-D as a carbon source but to an actual enhancement of the photosynthetic process. The uptake of radioactively labeled carbon (¹⁴c added as H¹⁴CO₃) was significantly increased in the presence of 2,4-D. The literature contains several reports of enzyme activity stimulation by 2,4-D (108). The data supported the suggestion that 2,4-D may influence the Hill reaction and respiration by stimulating an enzyme (or enzymes) involved in these processes. The net result may have been an increase in the rates of reactions in both the Calvin cycle and photorespiration. / Ph. D.

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Orientador: Dagoberto Martins / Coorientador: Roberto Antonio Rodella / Banca: Carlos Alberto Mathias Azania / Banca: Pedro Luis da Costa Aguiar Alves / Banca: Mario Sergio Tomazela / Banca: Luciano Soares de Souza / Resumo: O objetivo desse trabalho foi avaliar a seletividade dos herbicidas bentazon, nicosulfuron, halosulfuron, oxadiazon e 2,4-D aplicados em pós-emergência sobre as espécies Axonopus compressus (grama São Carlos), Paspalum notatum (grama Batatais), Stenotaphrum secundatum (grama Santo Agostinho) e Zoysia japonica (grama Esmeralda) e relacionar a seletividade destes herbicidas com a anatomia foliar destas espécies de gramas. As quatro espécies de grama apresentavam 48 meses de idade no momento da aplicação dos tratamentos. Os tratamentos constituíram-se pela aplicação dos herbicidas bentazon (720 g ha-1), nicosulfuron (50 g ha-1), halosulfuron (112,5 g ha-1), oxadiazon (875 g ha-1) e 2,4-D (698 g ha-1), além de uma testemunha sem aplicação, para cada espécie avaliada. O delineamento experimental utilizado foi em blocos ao acaso com quatro repetições. As avaliações de fitotoxidade das espécies de gramas foram realizadas a cada 7 dias após a aplicação dos herbicidas (DAA), até o desaparecimento dos sintomas. Para se proceder à análise anatômica das quatro espécies de gramas estudadas, o material foliar foi amostrado ao acaso na parcela, coletando-se as folhas inteiramente expandidas aos 7o, 21o e 35o dias após a aplicação dos herbicidas. Avaliou-se os caracteres quantitativos das regiões da nervura central (quilha) e da região compreendida entre a nervura central e a margem do limbo (asa). Os caracteres anatômicos foliares quantitativos analisados foram a porcentagem de epiderme das faces adaxial e abaxial, feixe vascular, bainha do feixe vascular, esclerênquima, parênquima, número e distância entre os feixes e espessura foliar. Avaliou-se também os caracteres morfológicos das gramas como a altura de planta, número e comprimento das inflorescências, número de estômatos e tricomas e massa... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The aim of this study was to evaluate the selectivity of the herbicides bentazon, nicosulfuron, halosulfuron, oxadiazon and 2,4-D applied in post-emergence on the Axonopus compressus (broadleaf carpetgrass), Paspalum notatum (bahiagrass), Stenotaphrum secundatum (St. Augustine grass) and Zoysia japonica (japanese lawn grass), and correlate the selectivity of these herbicides with the leaf anatomy of these species of grasses. The four species of grass had 48 months of age at the time of treatment application. The treatments were bentazon (720 g ha-1), nicosulfuron (50 g ha-1), halosulfuron (112.5 g ha-1), oxadiazon (875 g ha-1) and 2.4 D (698 g ha-1), and an untreated control for each species studied. The experimental design was randomized blocks design with four replications. Evaluations of phytotoxicity of species grasses were taken every seven days after herbicide application (DAA) until symptoms subside. To make the anatomical analysis of four species of grasses studied, the leaf material was sampled at random from the plot, collecting the expanded leaf leaves at 7, 21 and 35 days after herbicide application. It assessed the following quantitative traits regions of the midrib (keel) and the region between the midrib and the margin of the lamina (wing). The quantitative leaf anatomical characters were analyzed: the percentage of the adaxial epidermis and abaxial epidermis, vascular bundle, vascular bundle sheath, sclerenchyma, parenchyma, number and distance between the bundle vascular and leaf thickness. Evaluated the morphology of grasses such as plant height, number and length of inflorescences, number of stomata and trichomes and shoot dry matter. The results of phytotoxicity of herbicides on four species of... (Complete abstract click electronic access below) / Doutor

Orientador: Caio Antonio Carbonari / Banca: Edivaldo Domingues Velini / Banca: Anderson Luis Cavenaghi / Resumo: A presença de palha na superfície do solo pode alterar a dinâmica populacional das plantas daninhas presentes na área, além de influenciar o comportamento dos herbicidas no solo. Atualmente, pouco se sabe a respeito da dinâmica de liberação de herbicidas com efeito residual quando sua aplicação é realizada sobre a cobertura verde, no manejo em pré-semeadura da soja, mas supõe-se que sua distribuição e dinâmica sejam alteradas. O presente trabalho teve por objetivos avaliar o comportamento no solo, a eficácia no controle de plantas daninhas e a seletividade para a cultura dos herbicidas sulfentrazone e flumioxazin quando aplicados sobre dois tipos de coberturas em diferentes sistemas de manejo de dessecação em pré-semeadura da soja. Realizaram-se dois experimentos, no município de Botucatu, São Paulo, em áreas com coberturas de milheto e sorgo, com delineamento em blocos casualizados, com 7 tratamentos e 4 repetições. Os tratamentos foram constituídos pela aplicação de herbicidas em diferentes sistemas de manejo das culturas de cobertura, sendo eles: glyphosate (720 g e.a. ha-1) 20 dias antes da semeadura (DAS) + sulfentrazone (600 g i.a. ha-1) 10 DAS; glyphosate (720 g e.a. ha-1) 20 DAS + flumioxazin (50 g i.a. ha-1) 10 DAS; glyphosate (720 g e.a. ha-1) + sulfentrazone (600 g i.a. ha-1) 10 DAS; glyphosate (720 g e.a. ha-1) + flumioxazin (50 g i.a. ha-1) 10 DAS; sulfentrazone (600 g i.a. ha-1) 10 DAS sem a presença de cobertura; flumioxazin (50 g i.a. ha-1) 10 DAS sem a presença de cobertura e uma testemunha com aplicação de glyphosate (720 g e.a. ha-1) 10 DAS. Foram realizadas avaliações da disponibilidade de ... / Abstract: The presence of straw on the soil surface can alter the population dynamics of weeds present in the area, and influence the behavior of herbicides in soil. Currently, little is known about the dynamics of release of herbicides with residual effect when your application is performed on the green coverage in the management of pre-sowing soybean, but it is assumed that the distribution and dynamics are altered. This study aimed to evaluate the behavior in soil, the efficiency in weed control and selectivity to the culture of sulfentrazone and flumioxazin herbicide when applied over two types of covers in different desiccation management systems in pre-sowing soybean. There were two experiments, in Botucatu, São Paulo, in areas with millet and sorghum coverage, in a randomized block design with 7 treatments and 4 replications. The treatments were a combination of herbicides in different management systems of coverage crops, which are: glyphosate (720 g e.a. ha-1) 20 days before sowing (DBS) + sulfentrazone (600 g i.a. ha-1) 10 DBS; glyphosate (720 g e.a. ha-1) 20 DBS + flumioxazin (50 g i.a. ha-1) 10 DBS; glyphosate (720 g e.a. ha-1) + sulfentrazone (600 g i.a. ha-1) 10 DBS; glyphosate (720 g e.a. ha-1) + flumioxazin (50 g i.a. ha-1) 10 DBS; sulfentrazone (600 g i.a. ha-1) 10 DBS without the presence of coverage; flumioxazin (50 g i.a. ha-1) 10 DBS without the presence of cover and a control with application of glyphosate (720 g e.a. ha-1) 10 DBS. Were evaluated the availability of herbicides in the soil through periodic collections, weed control, weed survey, selectivity of herbicides to the crop, production and soybean yield components. For quantification of herbicides used a LCMS-MS system consisting of a High Performance Liquid Chromatography (HPLC) coupled with mass spectrometry. For flumioxazin, amounts of herbicide present in the samples were below the detection limit of the analytical method ... / Mestre

Orientador: Fernando Tadeu de Carvalho / Banca: Marcelo Carvalho Minhoto Teixeira Filho / Banca: Marco Antonio de Souza Silva / Resumo: Para o controle eficaz de plantas daninhas na cultura da cana-de-açúcar é pouco provável que uma única aplicação de herbicida em pré-plantio incorporado seja suficiente para manter a cultura no limpo até o seu fechamento. O objetivo do trabalho foi averiguar a eficácia (ou ineficácia) de herbicidas aplicados uma única vez em pré-plantio-incorporado para o controle de plantas daninhas em cana-de-açúcar. O delineamento experimental utilizado foi o de blocos ao acaso, com oito tratamentos e quatro repetições com parcelas de 30 m2 . A eficiência dos herbicidas no controle das plantas daninhas foi avaliada aos 30, 60, 90, 120 e 150 dias após a aplicação (DAA), por meio de uma escala visual, onde 0% = nenhum controle e 100% = controle total das plantas daninhas, considerando-se como eficiente o controle igual ou superior a 80%. Concluiu-se que todos os tratamentos (s-metolachlor a 1,5 e 3,0 L p.c./ha; smetolachlor+atrazin a 1,5 e 3,0 + 3,0 L p.c./ha; trifluralin a 2,0 L p.c./ha e trifluralin+atrazin a 2,0 + 3,0 L p.c./ha) foram altamente seletivos para a cultura da cana-de-açúcar, variedade RB 86-5453 e foram ineficazes para as dicotiledôneas Calopogonium muconoides (aos 150 DAA) e Amaranthus deflexus (aos 120 DAA) e, os tratamentos s-metolachlor+atrazin (1,5 e 3,0 + 3,0 L p.c./ha) e trifluralin+atrazin (2,0 + 3,0 L p.c./ha) foram eficientes para a espécie Panicum maximum. Todos os tratamentos proporcionaram menor crescimento e produtividade da canade-açúcar em relação à testemunha no limpo. Entre as testemunhas observou-se reduções no crescimento e principalmente na produtividade (55%) da testemunha sem capina em relação à capinada. Uma única aplicação dos herbicidas trifluralin, s-metolachlor, atrazin e suas combinações em pré-plantio incorporado, não foram suficientes para manter a cultura de canade-açúcar livre de mato-competição até o seu fechamento, indicando... / Abstract: To the weeds control in the sugarcane is unlikely that a single application of herbicide in preplanting-incorporated is sufficient to maintain the culture in clean until its closing. The objective of the work was to determine the effectiveness (or ineffectiveness) of herbicides applied only once in pre-plant-incorporated for weed control in sugar cane. The experimental design was a randomized block with eight treatments and four replications with plots of 30 m2 . The herbicides efficiency in weed control was assessed at 30, 60, 90, 120 and 150 days after application, through a visual scale, where 0% = no control and 100% = full control of plants weeds, considering how efficient control than or equal to 80%.It was concluded that all treatments (1.5 and 3.0 Lc.p./ha of s-metolachlor; 1.5 and 3.0 L + 3.0 Lc.p./ha of smetolachlor+atrazin; 2.0 Lc.p./ha of trifluralin and 2.0+3.0 Lc.p./ha of trifluralin+atrazin) were highly selective for the culture of sugar cane, variety RB 86-5453 and were ineffective for the dicotyledons Calopogonium muconoides (at 150 DAA) and Amaranthus deflexus (at 120 DAA) and, the treatments s-metolachlor + atrazin (1.5 and 3.0 + 3.0 Lc.p./ha) and trifluralin + atrazin (2.0 L + 3.0 c.p./ha) were effective for the species Panicum maximum. All treatments showed lower growth and yield compared to the control in the clean. Among the controls was observed reductions in growth and 55% reduction in the productivity of the treatment without controlling weeds in relation to treatment weeded. A single application of trifluralin herbicides, smetolachlor, atrazine and their combinations in corporate pre-planting, were not enough to keep the culture of sugarcane free of weed competition until its closure, indicating a need for other methods subsequent control / Mestre