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by Chan Po Wai. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1997. / Includes bibliographical references (leaves 110-117). / Table of Contents --- p.i / Abbreviations --- p.iv / Abstract --- p.v / List of Figures --- p.vii / List of Tables --- p.xi / Chapter Chapter One: --- Introduction / Chapter 1.1 --- Objective and scope of the project --- p.1 / Chapter 1.2 --- Literature review --- p.3 / Chapter 1.2.1 --- Balkan endemic nephropathy --- p.3 / Chapter 1.2.2 --- Chinese herbs nephropathy --- p.4 / Chapter 1.2.3 --- Aristolochic acid --- p.6 / Chapter 1.2.4 --- Guan-mu-tong --- p.9 / Chapter 1.2.4.1 --- Plant --- p.9 / Chapter 1.2.4.2 --- Traditional uses --- p.10 / Chapter 1.2.4.3 --- Chemical constituents --- p.11 / Chapter 1.2.4.4 --- Pharmacological study --- p.11 / Chapter 1.2.4.5 --- Reported adverse cases --- p.12 / Chapter 1.2.5 --- Ma-dou-ling --- p.12 / Chapter 1.2.5.1 --- Plant --- p.12 / Chapter 1.2.5.2 --- Traditional uses --- p.13 / Chapter 1.2.5.3 --- Chemical constituents --- p.14 / Chapter 1.2.5.4 --- Clinical and pharmacological studies --- p.14 / Chapter 1.2.5.5 --- Reported adverse cases --- p.15 / Chapter 1.3 --- Chemical analysis --- p.16 / Chapter 1.3.1 --- Thin layer chromatography --- p.16 / Chapter 1.3.2 --- High performance liquid chromatography --- p.17 / Chapter 1.4 --- Toxicology --- p.18 / Chapter 1.4.1 --- Acute toxicity --- p.18 / Chapter 1.4.2 --- Subchronic toxicity --- p.19 / Chapter 1.4.3 --- Reproductive toxicity --- p.23 / Chapter Chapter Two: --- Materials & Methods / Chapter 2.1 --- Materials --- p.24 / Chapter 2.2 --- Methods --- p.27 / Chapter 2.2.1 --- "Aqueous extraction of Guan-mu-tong and Ma-dou-ling for acute, subchronic and reproductive toxicity tests" --- p.27 / Chapter 2.2.2 --- Chemical analysis --- p.28 / Chapter 2.2.2.1 --- Thin layer chromatography --- p.28 / Chapter 2.2.2.2 --- High performance liquid chromatography --- p.28 / Chapter 2.2.3 --- Assays for the toxicity --- p.30 / Chapter 2.2.3.1 --- Acute toxicity --- p.30 / Chapter 2.2.3.2 --- Subchronic toxicity --- p.31 / Chapter 2.2.3.3 --- Reproductive toxicity --- p.32 / Chapter 2.2.4 --- Statistical analysis --- p.33 / Chapter Chapter Three: --- Results / Chapter 3.1 --- Chemical Analysis --- p.34 / Chapter 3.1.1 --- Thin layer chromatography --- p.34 / Chapter 3.1.2 --- High performance liquid chromatography --- p.34 / Chapter 3.2 --- Toxicity of Guan-mu-tong --- p.42 / Chapter 3.2.1 --- Acute toxicity --- p.42 / Chapter 3.2.2 --- Subchronic toxicity --- p.44 / Chapter 3.2.3 --- Reproductive toxicity --- p.54 / Chapter 3.3 --- Toxicity of Ma-dou-ling --- p.56 / Chapter 3.3.1 --- Acute toxicity --- p.56 / Chapter 3.3.2 --- Subchronic toxicity --- p.66 / Chapter 3.3.3 --- Reproductive toxicity --- p.89 / Chapter Chapter Four: --- Discussion / Chapter 4.1 --- Chemical Analysis --- p.91 / Chapter 4.1.1 --- Thin layer chromatography --- p.91 / Chapter 4.1.2 --- High performance liquid chromatography --- p.91 / Chapter 4.2 --- Toxicity of Guan-mu-tong --- p.93 / Chapter 4.2.1 --- Acute toxicity --- p.93 / Chapter 4.2.2 --- Subchronic toxicity --- p.93 / Chapter 4.2.3 --- Reproductive toxicity --- p.94 / Chapter 4.3 --- Toxicity of Ma-dou-ling --- p.95 / Chapter 4.3.1 --- Acute toxicity --- p.95 / Chapter 4.3.2 --- Subchronic toxicity --- p.97 / Chapter 4.3.3 --- Reproductive toxicity --- p.105 / Chapter Chapter Five: --- Conclusion --- p.107 / Bibliography --- p.110 / Appendix A: Procedure on determining the total urinary protein --- p.119 / Appendix B: Procedure on determining the total urinary glucose using Sigma diagnostic kits --- p.121 / Appendix C: Procedure on determining the activity of aspartate aminotransferase --- p.123 / Appendix D: Procedure on determining the activity of alanine aminotransferase --- p.124 / Appendix E: Procedure for preparing a calibration curve for the measurement of aspartate aminotransferase and alanine aminotransferase activities --- p.125 / Appendix F: Procedure on tissue preparation for light microscopic study --- p.128

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