Pluchea indica (L.) Less. is a shrub of the family Compositae and is widespread along the western coast in Taiwan. Previous studies indicated that the components of Pluchea indica have potent anti-inflammatory, anti-ulcer and antimicrobial activities. In the present study, the effects of aqueous extract of roots and leaves from P. indica on the cancer cell lines were investigated. Various experimental approaches including cell growth curves, MTT assay, MTS assay, focus formation assay and cell migration assay were performed on the aqueous extract-treated cancer cells. Our results demonstrate the aqueous extract of P. indica induced anti-proliferation activity on GBM8401 and HeLa cancer cell lines.