The introduction of species outside of their native ranges has been extensively studied in ecology. Particular attention has been paid to examining interactions between alien and native plants, and a large proportion of this attention has focused on pollinator-mediated interactions. In order to interact through pollinators, plants must co-occur, coflower, and share pollinators; studies investigating pollinator-mediated interactions between alien and native plants frequently make fundamental assumptions about the definition of these prerequisites to pollinator-mediated interaction. The present analysis examines assumptions about plant co-occurrence and the effects that these assumptions have on study outcomes. In Chapter 2, I present the results of a meta-analysis of 76 studies which overturns previous findings that pollinator-mediated interactions between plants can be predicted on the basis of whether the neighbour is an alien, phylogenetic distance, or floral trait similarity. Moreover, I demonstrate that the spatial definition of the control group (i.e. the distance between the group of focal plants that ‘do not co-occur’ with the alien/alternate neighbour species and the nearest individuals of that neighbour species), and the spatial arrangements of plants within their treatment groups (i.e. the relative placement of the group of focal plants that ‘co-occurr’ with the neighbour species), both have a significant impact on the outcome of pollinator-mediated interactions between alien and native plants. I also emphasize evidence of bias in the selection of study systems and in the process of publication. In Chapter 3, I present the results of a field experiment testing the role of distance between interacting plants in determining patterns of visitation by insects. The results of this analysis are suggestive of visitor functional group-dependent effects but limited by low power. In both Chapter 2 and Chapter 3, I show that heretofore unexamined assumptions about definitions of co-occurrence of plants may be introducing bias into studies of pollinator-mediated interactions between plants, and that facilitation and competition between plants for visitation may be linked across different spatial scales.

The ecological dynamics of co-flowering communities are largely mediated by pollinators. However, current understanding of pollinator-mediated interactions primarily relies on how co-flowering plants influence attraction of shared pollinators, and much less is known about plant–plant interactions that occur via heterospecific pollen (HP) transfer. Invaded communities in particular can be highly affected by the transfer of alien pollen, but the strength, drivers and fitness consequences of these interactions at a community scale are not well understood. Here we analyse HP transfer networks in nine coastal communities in the Yucatan Mexico that vary in the relative abundance of invasive flowers to evaluate how HP donation and receipt varies between native and alien plants. We further evaluate whether HP donation and receipt are mediated by floral traits (e.g. display, flower size) or pollinator visitation rate. Finally, we evaluated whether post-pollination success (proportion of pollen tubes produced) was affected by alien HP receipt and whether the effect varied between native and alien recipients. HP transfer networks exhibit relatively high connectance (c. 15%), suggesting high HP transfer within the studied communities. Significant network nestedness further suggests the existence of species that predominantly act as HP donors or recipients in the community. Species-level analyses showed that natives receive 80% more HP compared to alien species, and that alien plants donate 40% more HP than natives. HP receipt and donation were mediated by different floral traits and such effects were independent of plant origin (native or alien). The proportion of alien HP received significantly affected conspecific pollen tube success in natives, but not that of alien species. Synthesis. Our results suggest that HP transfer in invaded communities is widespread, and that native and alien species play different roles within HP transfer networks, which are mediated by a different suite of floral traits. Alien species, in particular, play a central role as HP donors and are more tolerant to HP receipt than natives—a finding that points to two overlooked mechanisms facilitating alien plant invasion and success within native co-flowering communities.

The ecological dynamics of co-flowering communities are largely mediated by pollinators. However, current understanding of pollinator-mediated interactions primarily relies on how co-flowering plants influence attraction of shared pollinators, and much less is known about plant–plant interactions that occur via heterospecific pollen (HP) transfer. Invaded communities in particular can be highly affected by the transfer of alien pollen, but the strength, drivers and fitness consequences of these interactions at a community scale are not well understood. Here we analyse HP transfer networks in nine coastal communities in the Yucatan Mexico that vary in the relative abundance of invasive flowers to evaluate how HP donation and receipt varies between native and alien plants. We further evaluate whether HP donation and receipt are mediated by floral traits (e.g. display, flower size) or pollinator visitation rate. Finally, we evaluated whether post-pollination success (proportion of pollen tubes produced) was affected by alien HP receipt and whether the effect varied between native and alien recipients. HP transfer networks exhibit relatively high connectance (c. 15%), suggesting high HP transfer within the studied communities. Significant network nestedness further suggests the existence of species that predominantly act as HP donors or recipients in the community. Species-level analyses showed that natives receive 80% more HP compared to alien species, and that alien plants donate 40% more HP than natives. HP receipt and donation were mediated by different floral traits and such effects were independent of plant origin (native or alien). The proportion of alien HP received significantly affected conspecific pollen tube success in natives, but not that of alien species. Synthesis. Our results suggest that HP transfer in invaded communities is widespread, and that native and alien species play different roles within HP transfer networks, which are mediated by a different suite of floral traits. Alien species, in particular, play a central role as HP donors and are more tolerant to HP receipt than natives—a finding that points to two overlooked mechanisms facilitating alien plant invasion and success within native co-flowering communities.