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An investigation into the biological activity of rooibos (Aspalathus linearis) extractsRichfield, David 03 1900 (has links)
Thesis (MSc (Biochemistry))--Stellenbosch University, 2008. / This study describes:
1. The preparation of chloroform, methanol and aqueous extracts of
unfermented and fermented rooibos (Aspalathus linearis).
2. The chromatographic fractionation of aqueous rooibos extracts and an
investigation into the polyphenol content and antioxidant activity of
the fractions.
3. The preparation of ovine adrenal microsomes containing active
steroidogenic P450 enzymes, including cytochrome P450
17a-hydroxylase, CYP17, and cytochrome P450 steroid
21-hydroxylase, CYP21.
4. An investigation into the influence of chloroform and methanol
extracts of rooibos on the binding of steroid substrates, progesterone
and 17-hydroxyprogesterone, to CYP17 and CYP21.
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