Field experiments were conducted in two different growing environments to evaluate the spread and movement of Dickeya dadantii. A procedure to inoculate seed potatoes with Dickeya dadantii was developed to use during this study. Spread of Dickeya dadantii from inoculated potato seed to healthy potato seed during the handling, cutting and planting procedures was not detected at either location. Spread of Dickeya dadantii from inoculated seed to surrounding progeny tubers in the field was documented in both locations. In Florida, 33% of progeny tubers tested positive for Dickeya using PCR, and in North Dakota, 13% of the progeny tubers tested positive. Stunting was observed in plants grown from Dickeya dadantii inoculated seed tubers in North Dakota, but not in Florida. These results indicate that Dickeya dadantii may spread during the seed handling and cutting processes and can spread in the field from infected seed tubers to progeny tubers.

In the past 10 years we have witnessed the beginnings of the study of soil ecology as a unified science, and the general realization by soil scientists, farmers, and land managers that many of the most important economic aspects of soil health are controlled by biological factors. This research focuses on alterations in a tropical soil microarthropod community under differing intensive agricultural protocols: native vegetation, pastures and cropland, during June, July and August 1998. The effect of pesticides in potato cultivation was also studied. In the Ecuadorean montane forest, 361 morphospecies of soil arthropods, were classified during the three sampling months. August was the month with highest abundance and diversity. Acari, Coleoptera, Collembola, Diptera and Homoptera were the most abundant orders present in all the three types of land management. The native vegetation had the most abundant and diverse representation of all soil arthropod taxa compared to the pastures and croplands. Coleoptera, Diptera, Diplopoda, Diplura and Hemiptera were significantly more diverse in native vegetation than in pastures and croplands. The most abundant functional groups were the fungivores, herbivores and predators. The abundance of functional groups was significantly higher in the native vegetation for predators, herbivores and detritivores. 115 morphospecies of soil arthropods were identified in the study of arthropod response to pesticides in a complete randomized potato plot. Seasonal effects were documented for Acari, Collembola, Diptera, and Homoptera. Predators were most abundant in July and fungivores decreased in September. Neither arthropod orders nor functional groups showed a significant change in abundance between different treatments. Only Homoptera showed an increase in its abundance in the third sampling date and only in the Antracol plots. The potato plants in the whole block showed poor productivity, suggesting that the whole system was stressed by the fungal pest. / Graduation date: 2000

No description available.

The potential of hot water dipping, vapour heating or ultraviolet irradiation to eliminate the use of chemicals for control of sprouting and post-harvest diseases of the potato (Solanum tuberosum L.) was examined. The microorganisms on which these treatments were tested were the fungal dry rot (Fusarium solani) and the bacterial soft rot (Erwinia carotovora pv. carotovora), two major post-harvest pathogens of potatoes. The study focused on short-term storage (three months) at 8$ sp circ$C or 18$ sp circ$C, which are representative of storage temperatures used by producers in northern temperate and semi-arid tropical regions, respectively. / Response surface methodology was used in experimentation to facilitate analysis of data and identification of optimal operating conditions for the treatments. The following parameters were used to assess tuber quality after the treatments and 3-month storage: firmness, color and structure. / It was found possible to obtain 100% control of sprouting and diseases for the three-month storage without resorting to the use of chemicals. This was achieved without significantly altering the quality attributes of the tubers under certain conditions of hot water, ultraviolet radiation or combinations of these two with storage at 8$ sp circ$C for three months. Although 100% control was not possible for the storage at 18$ sp circ$C, treated tubers performed much better than the controls. Vapour heat (50-70$ sp circ$C) was much less effective at controlling sprouting and was therefore not tested on the pathogens. / A numerical model of the heat transfer phenomenon in the tuber was also developed. It was used to predict the transient temperature distribution in the tuber. The model was solved using the line-by-line technique and model simulations were validated against experimental data.

No description available.

Please read the abstract in the section 00front of this document. Also note that an abstract is provided for each chapter as well / Thesis (DPhil (Entomology))--University of Pretoria, 2005. / Zoology and Entomology / unrestricted

No description available.

Maize grey leaf spot (GLS), caused by Cercospora zeae-maydis, and potato late blight (LB), caused by Phytophthora infestans, are foliar diseases of maize and potato, two of the most widely grown crops in KwaZulu-Natal (KZN), after sugarcane and timber. Commercial maize in KZN accounts for just on 4.3% of the national maize crop. This is worth R563 million using an average of the yellow and white maize price for the 2001/02 season (at R1 332.87 ton(-1)). In 2003 KZN produced about 5% of the national potato crop (summer crop: 7531 300 10kg pockets from 2243 hectares). This equates to a gross value of R89.4 million based on an average price of R1 188 ton(-1) in 2001. Successful commercial production of maize and potatoes depends upon control of these diseases by translaminar fungicides with highly specific modes of action. This study extends an existing model available for timing of fungicide sprays for GLS and tests and compares two LB models for two calendar-based spray programmes. The study also evaluated the use of an early blight model which is caused by Alternaria solani, and over the single season of evaluation showed potential for use in KZN. For the GLS model it was found that a number of refinements are needed, e.g., the amount of infected maize stubble at planting and not the total amount of maize residue at planting. Based on two years' data, it was found that for the LB models there are no significant differences in levels of control between using a predicted fungicide programme and a calendar-based programme. The importance of knowing initial infection sites, and hence initial inoculum, was demonstrated. This led to the creation of a KZN LB incidence map, now being used to more accurately time the start of a preventative spray programme and to time the inclusion of systemic fungicides in the preventative spray programme. This study has contributed to the further development and expansion of the Automatic Weather Station Network (AWSN) at Cedara, which now comprises 15 automatic weather stations in KZN. The AWSN is currently used to aid farmers and advisers in decision-making regarding fungicide spray timing for GLS and LB. / Thesis (M.Sc.Agric.)-University of Natal, Pietermaritzburg, 2003.