It is essential for prey species to be able to detect predators to avoid them. The sense of smell is used by a number of prey species for this purpose. The aim of the present study was to assess if one of the odourants that make up a predator odour is sufficient to induce a behavioural response in mice (Mus musculus). Two predator odourants were used, 2,2-dimethylthietane and methyl-2-phenylethyl sulfide, which are both found in the secretions of natural predators of mice. An odourant found in fruits, n-pentyl acetate, was also used. All three odourants were presented at a concentration that was a factor of 100 above the olfactory detection threshold of mice. Ten adult predator-naïve CD-1 mice were individually put in a two-compartment chamber one of which contained an odourant while the other contained a near-odourless solvent (diethyl phthalate). The results indicated that methyl-2-phenylethyl sulfide was actively avoided by the mice. Towards 2,2-dimethylthietane and n-pentyl acetate, in contrast, the mice behaved indifferent. Further, the results suggest a significant correlation between the number of switches between the two compartments of the test chamber and the test sessions when the animals were presented with n-pentyl acetate, but not when they were presented with the predator odourants. The results support the notion of an innate fear response towards the predator odourant methyl-2-phenylethyl sulfide in mice, but further studies with more animals and different concentrations of the odourants are necessary.