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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Gene regulatory networks controlling an epithelial-mesenchymal transition

Wu, Shu-Yu 03 May 2007 (has links)
Epithelial-mesenchymal transitions (EMTs) are fundamental and indispensable to embryonic morphogenesis throughout the animal kingdom. At the onset of gastrulation in the sea urchin embryo, micromere-derived primary mesenchyme cells (PMCs) undergo an EMT process to ingress into the blastocoel, and these cells later become the larval skeleton. Much has been learned about PMC specification in sea urchin embryos. However, much less is known about how states of the sequentially progressing PMC gene regulatory network (GRN) controls the EMT process during PMC ingression. Transcriptional regulators such as Snail and Twist have emerged as important molecules for controlling EMTs in many model systems. Sea urchin snail and twist genes were cloned from Lytechinus variegates, and each has been experimentally connected to the PMC regulatory network; these experiments demonstrate several requirements for PMC ingression, and in doing so, begin to illustrate how a gene regulatory network state controls morphogenesis. Functional knockdown analyses of Snail with morpholino-substituted antisense oligonucleotides (MASO) in whole embryos and chimeras demonstrated that Snail is required in micromeres for PMC ingression. Investigations also show that Snail downregulates cadherin expression as an evolutionarily conserved mechanism, and Snail positively regulates a required endocytic clearance of epithelial membrane molecules during EMT. Perturbation experiments indicate that Twist has accessory roles in regulating PMC ingression, and possibly plays a maintenance role in PMC specification network state. In addition, Twist also functions in the post-EMT network state, particularly in directing PMC differentiation and skeletogenesis. The recently annotated sea urchin genome accelerates the discovery of new genes and holds strong promise of mapping out a complete canvas of the micromere-PMC gene regulatory network. Using the genome resources we successfully cloned several newly identified PMC genes, and found most of them to be expressed in micromeres just prior to ingression of the nascent PMCs. Current experiments focus on the roles of these genes in preparing for, executing, and/or controlling the mesenchymal behavior following PMC ingression. The functions and inter-relationships of these genes will greatly augment our understanding of how a gene regulatory network state controls a crucial morphogenetic event. / Dissertation
2

Custom biomineral production using synthetic embryonic tissue

Cao, Yi 04 October 2022 (has links)
Continuous efforts have been directed towards controlled calcium carbonate biomineral synthesis in recent years. Compared to their inorganic counterparts, biominerals are more tensile in industrial applications, biocompatible with scientific designs, and sustainable for the environment. Most current approaches for synthetic biomineral production rely heavily on sophisticated engineering techniques to constrain the physical property of their crystals, which limits the adaptability of these products. Here, we proposed a novel approach to synthesize calcium carbonate biominerals by reproducing skeletogenesis of the sea urchin larva in vitro using common cellular and molecular methods. Skeleton formation in Lytechinus variegatus sea urchin embryos is a highly coordinated event, where ectodermal cells in different domains express distinct patterning cues that are received by adjacent primary mesenchyme cells (PMCs), which in turn secrete the skeleton. Our group and others have identified a range of skeletal patterning cues, and based on our current understanding of the mechanism, we envisioned a synthetic ectoderm culture using defined ectodermal lineages that, when combined with PMCs, will direct the synthetic production of skeletal structures. Here we have developed a detailed protocol for establishing such as ectoderm culture and have begun initial experiments towards this goal. Future deployment of this protocol will provide invaluable insights into the mechanism of skeletal patterning in sea urchins, as well as an unprecedented system for customized synthetic calcium carbonate biomineral production. Finally, improving our mechanistic understanding of skeletal patterning in echinoderms has the potential to shed light on analogous biomineralization processes in other species as well. / 2024-10-03T00:00:00Z

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