Brassica carinata (A.) Braun and Camelina sativa (L.) Crantz are two re-emerging oilseed crops of the Brassicaceae family that are being adapted for cultivation in western Canada. Both seeds of these species reportedly accumulate considerable amounts of sulfur-containing secondary metabolites called glucosinolates. The purpose of the current work was to gain knowledge of the occurrence and distribution of glucosinolates during primary processing of these oilseeds, including during pressing and dehulling. In the first study, a reversed phase HPLC method was developed for the analysis of sinigrin, the major glucosinolate in B. carinata. Both C18 columns selected were able to separate the compound with an isocratic eluent containing 100% tetramethylammonium bromide (10 mM, pH 5) delivered at 1 mL/min at a column temperature of 25oC. These chromatographic conditions were applied and sinigrin concentration of whole B.carinata seed was estimated to be 29 μg/mg. Average matrix effect was estimated to be 104% that was caused by other components in the B. carinata seed matrix. In the second study, high concentrations of glucosinolates were detected and identified in fractions of C. sativa seeds using HPLC-ESI-MS. Methods for extraction, isolation, and purification of three individual glucosinolates from these fractions are reported. Quantitation of total glucosinolates was performed on proton NMR using DMF as an internal standard. Quantitation of individual glucosinolates was achieved by using MS extracted ion chromatogram data. Total glucosinolates were found in C. sativa whole seed at a concentration of 14 μg/mg, and glucocamelinin, the major glucosinolate, constituted 65% of the total amount. In addition, a dehulling treatment was applied to C. sativa seeds, from which both oil content and crude protein content increased after dehulling of the seeds.

Producing oil is a complex process where the fluids existing in a deep layer of the subsurface must be raised to the surface through the production well, where oil and natural gas are handled. The primary treatment of oil is basically done in two stages, the separation of gaseous and liquid phase separation, oil, water and other contaminants. Production facilities in offshore fields, which concentrates the largest and most recent discoveries of oil, the relatively small space in the production platforms has led to the employment of more compact process equipment. Pressure vessels are used in the separation of liquid phases, oil and water, imposing greater turbulence to fluids contributing to the formation and stabilization of emulsions of oil. This work aims at using CFD (Computational Fluid Dynamics) a software tool to visualize the behavior of fluid flow to analyze the dynamics of atmospheric flow in gravitational separators. To evaluate the influence of some internal atmospheric gravity separator tank known as Free Water Tank and how some its dimensions interfere with the gravitational separation. We restrict our analysis in a qualitative view of fluid dynamics in the gravitational separator and the occurrence of atmospheric phenomena that may influence its efficiency. For the separator model we could highlight the importance of the inductor tube, since the velocity profiles produced show the influence of its dimensions in the functionality of the equipment. Therefore the gravitational separators type, Free Water Tank height and diameter of the tube inducer, greatly influence the performance of the gravitational separator, thus, appears that supporting devices should be objects of analysis also at existing facilities. / Produzir petróleo é um processo complexo, no qual os fluidos existentes em uma camada profunda do subsolo devem ser elevados através do poço produtor, até a superfície, onde o petróleo e o gás natural são tratados. O tratamento primário do petróleo é realizado basicamente em dois estágios: a separação da fase gasosa e a separação da fase líquida constituída de petróleo, água e outros contaminantes. Nas unidades de produção em campos marítimos, onde se concentram as maiores e mais recentes descobertas de petróleo, o espaço físico relativamente pequeno nas plataformas de produção tem induzido ao emprego de equipamentos de processo mais compactos. Vasos de pressão são utilizados na separação das fases líquidas, nos quais ocorre grande movimentação dos fluidos, o que contribui fortemente para a formação e estabilização das emulsões de petróleo. Este trabalho objetiva com o uso de CFD (Computacional Fluid Dynamics) uma ferramenta computacional que permite visualizar o comportamento de fluidos em movimento apresentar uma análise da dinâmica de fluxo em separadores gravitacionais atmosféricos. Faz-se a avaliação da influência de alguns internos de um separador gravitacional atmosférico conhecido como Tanque de Água Livre (TAL), verificando-se como algumas dimensões dos mesmos interfere na separação gravitacional. Este trabalho restringiu-se a uma análise da dinâmica dos fluidos no separador gravitacional atmosférico e à ocorrência de fenômenos que possam influir na sua eficiência. Para o separador modelo pôde-se evidenciar a importância do tubo indutor, uma vez que os perfis de velocidade produzidos mostram a influência das dimensões deste dispositivo no desempenho do equipamento. Constatou-se que nos separadores do tipo Tanque de Água Livre a altura e o diâmetro do tubo indutor influenciam sensivelmente no desempenho do separador gravitacional, denotando a importância de analisar o efeito que estes dispositivos coadjuvantes podem ter nas instalações existentes para o tratamento primário de petróleo.