As concerns regarding the growing human population, rising seafood demand, and up to present limited success of fisheries management intensify, aquaculture is increasingly posited as a means to more efficiently produce seafood commodities. However, aquaculture expansion raises contentious issues itself. The current study addresses a number of these issues in attempts to improve hatchery production and related activities. Investigation of less-harmful chemotherapeutants for disease reduction at the egg stage could lead to improved hatchery microbial management, increased survival during early larval stages, increased efficacy of bacterial probiotic incorporation, and reductions in disease transfer between hatcheries and locations. Attempts to surface sterilize and disinfect cobia Rachycentron canadum eggs with 3 and 2% hydrogen peroxide solutions significantly reduced survival through the yolk-sac larvae stage. Furthermore, timing of treatment application at differing stages of egg development was found to significantly impact survival, highlighting the importance of this compounding factor. Bacterial probiotics can remediate water quality, reduce target host stress, and improve survival and population growth rates of live feed organisms. Two of the following studies investigated the benefits of incorporation of a Bacillus spp. probiotic blend in aquaculture activities. During closed container mock shipment of yellowfin tuna Thunnus albacares yolk-sac larvae, significant reductions in total ammonia nitrogen resultant from probiotic incorporation were observed. Furthermore, significantly reduced dissolved oxygen utilization might have resulted from stress reduction, as suggested by a non-significant lower degree in the breakdown of osmoregulation of the larvae. Incorporation of probiotics within rotifer Brachionus plicatilis cultures resulted in significantly higher daily mean populations, significantly lower population variability among replicates, and non-coincident logistic population growth regressions which yielded higher population growth rates and system carrying capacities. Broodstock management should not only maximize quantity of reproductive output, but quality as well. Significant increases in cobia egg diameter with increasing broodstock female age were detected, as well as significant increases in egg diameter with decreasing salinity. Confirmation of potentially increased larval growth rates resultant from increased energy stores of larger eggs when produced by older females, as demonstrated in other species, is warranted. Attempts to find production parameters which maximize cobia larval growth and survival would lead to increased sustainability via reducing demand for wild-collected Artemia and economic efficiency. Gaps in knowledge for achieving this goal continue to exist, but conclusions drawn from analysis of multiple production scale trials indicate temperatures of 29-31 °C maximize growth and survival. Improvement in sampling design and data analysis would increase statistical rigor and ease comparability of larvicultures outcomes across ranging influential factors. Further investigation of all of these matters is certainly warranted, although conclusions drawn could be effectively utilized to improve success of hatchery operations.

Les probiotiques sont des microorganismes vivants qui, lorsqu’ils sont ingérés en quantité adéquate, peuvent exercer des propriétés bénéfiques sur la santé de l’hôte. Les souches de Bacillus utilisées en tant que probiotiques ne sont pas colonisatrices du tractus intestinal, mais sont des résidents transitoires du microbiote. Ce travail fait l’investigation in vitro de l’association, qualifiée de synbiotique, entre une souche probiotique de Bacillus subtilis et une source carbonée prébiotique, composé alimentaire peu digéré par les enzymes intestinales mais utilisable par les bactéries dans l’intestin. L’étude de cette association met en évidence la capacité de la souche à utiliser les isomaltooligosaccharides (IMOS) prébiotiques comme unique substrat carboné. L’effet positif de ce substrat sur la tolérance à la bile de la souche a été démontré in vitro. Les résultats d’une analyse protéomique faisant l’étude des enzymes clés impliquées dans le métabolisme des IMOS, ainsi que d’autres biomarqueurs d’intérêt probiotique, sont en cours d’exploitation. Ce travail préliminaire d’investigation de l’association synbiotique entre les IMOS prébiotiques et la souche probiotique B. subtilis, aboutira à des essais in vivo. Les effets bénéfiques des probiotiques du genre Bacillus, notamment au niveau de la modulation du système immunitaire, résultent de l’interaction entre les molécules de la surface bactérienne et les cellules de l’intestin. Les mécanismes moléculaires à l’origine de l’immunomodulation sont mal connus, alors que leur compréhension est nécessaire à l’optimisation de l’utilisation du probiotique. Un deuxième volet de ce travail concerne la comparaison des structures d’entités moléculaires de surface de trois Bacilli probiotiques, les acides lipotéichoïques (LTAs), et leurs activités immunologiques respectives. Une étude structurale des LTAs par des méthodes biochimiques et par RMN a permis de mettre en évidence la diversité structurale au sein du même genre Bacillus. Le rôle clé de la D-alanine dans l’activité biologique de ces antigènes bactériens a été démontré. / Probiotics are live microorganisms, which when administered in adequate amounts confer a health benefit on the host. Bacillus probiotic strains are not able to colonize the gut, and are considered as transient residents of the microbiota. Prebiotic are non-digestible food ingredients that could stimulate growth of bacteria in the gut. This work investigates the in vitro effect of a prebiotic isomaltooligosaccharide (IMOS) on the growth of a probiotic strain Bacillus subtilis. This study highlights the ability of the strain to use IMOS as unique carbon source. A comparative proteomic analysis investigates the main enzymes implicated in the prebiotic metabolism, and biomarkers possibly involved in probiotic effects. This preliminary work, which studies the synbiotic association between a probiotic and a prebiotic, will lead to in vivo assays. Beneficial effects of probiotic Bacilli, mainly modulation of intestinal immune system, result from interaction between bacterial cell-wall molecules and intestinal cells. The molecular origin of immunomodulatory mechanisms are poorly understood, while understanding is needed to optimize the use of probiotics. A second part of this work consists in comparing the structure of a molecular cell-wall component named lipoteichoic acids (LTA) from three Bacillus probiotic, a molecular cell-wall component of Gram positive bacteria, and their immunological activity. A structural study, using biochemical determinations and NMR spectroscopic analysis, highlights the structural diversity between LTAs from different Bacillus species. The key role of D-alanine substituents in the biological activity of these bacterial antigens has been demonstrated.

Background: Almost all foals develop transient diarrhoea within the first weeks of life. Studies indicated different viral, bacterial, and parasitic causes, such as rotavirus, Clostridium perfringens, Escherichia coli, and Cryptosporidium are discussed. But little is known about the development of intestinal microflora in foals. The present study investigated whether the supplementation with Bacillus cereus var. toyoi would modify the developing intestinal microflora and consequently reduce diarrhoea in foals. From birth, the foals were randomly assigned to three treatment groups: placebo (10 mL isotonic NaCl, n = 8), low dosage (LD; 5 × 108 cfu B. cereus var. toyoi, n = 7) and high dosage (HD; 2 × 109 cfu B. cereus var. toyoi, n = 10). Treatment groups were supplemented orally once a day for 58 days. Faeces scoring and sampling were performed within the first 24 h after birth and on day 9, 16, 23, 30, 44, 58 of the foal’s life and also on the first day of diarrhoea. Culture-plate methods were used to analyse the bacterial microflora. Results: Eighty-eight per cent of the foals developed diarrhoea (placebo 7/8, LD 5/7, HD 10/10) during the first 58 days of life. Bacillus cereus var. toyoi supplementation had no effect on bacterial microflora. Clostridium perfringens and enterobacteria were equally prevalent in foals with diarrhoea and those who were not afflicted. Conclusions: We conclude that the supplementation of B. cereus var. toyoi had no effect on the occurrence of diarrhoea and health status in the foals.