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11	Příprava rekombinantních forem extracelulární domény myších leukocytárních receptorů z rodiny NKR-P1. / Preparation of recombinant forms of the extracellular part of mouse leukocyte receptors from NKR-P1 family. Adámek, David January 2012 (has links) Mouse NK cell receptors belonging to NKR-P1 family plays role in activation, inhibition and cytokine secretion by these cells. Aim of this thesis is preparation of extracellular parts of C57BL/6 mouse strain activating receptors mNKR-P1A and mNKR-P1C. Production vectors with coding sequences of both proteins were prepared. Next, optimization of production in E. coli was done and appropriate in vitro refolding and purification protocol were developed. Purified proteins were characterized by mass spectrometry and labeled by a fluorescent dye. Primary screening for potential ligand was performed. Further work will involve structural characterization of the receptors and identification of their ligands. These data may help to clarify the function of NK cells.
12	Studium interakce lektinových receptorů přirozených zabíječů s jejich proteinovými ligandy. / Studies on interactions between natural killer cell lectin receptors and their protein ligands. Hernychová, Lucie January 2014 (has links) NK cells are innate lymphocytes which constitute the first line of organism's defence against infections through their receptor system. These cells represent an important part of antiviral and antitumor immunity, they also play a role in transplant immunity, autoimmunity and reproduction. This diploma thesis inquires into the structure of the transmembrane receptor NKR-P1B of mouse NK cells and the interaction with its ligand Clr-b. The aim was to prepare the expression vector coding the ligand-binding and whole extracellular region of the receptor NKR-P1B and to optimize its production and refolding in vitro. Purified protein samples were analyzed by size-exclusion chromatography, electrophoresis and mass spectrometry. Interaction between NKR-P1B and Clr-b proteins was tested using biophysical (size-exclusion chromatography and surface plasmon resonance) and biological methods (labelling of cellular sample with NKR-P1B proteins marked with fluorescent dye). In vitro binding experiments have not confirmed mutual interaction between NKR-P1B and Clr-b despite the prepared proteins binding to the bone marrow cells.
13	Electromagnetic field and neurological disorders Alzheimer´s disease, why the problem is difficult and how to solve it Lyttkens, Peter January 2018 (has links) No description available. Alzheimer oxidative stress EMF microwave VGCC ACh protein refolding APOE big data FINGER wifi DECT AChE DNA-damage mobile phone glucose metabolism demyelinating antioxidant defence defective proteins non thermal effects Alzheimer risk pathophysiology mechanism amyloid pathologies multifactor strategy diet exercise cognitive training vascular risk management exposure sleep disturbance circadian dysfunction neuropsychiatric symptom risk assessments Neurosciences Neurovetenskaper

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