Thesis (Ph. D.)--University of Queensland,2001. / Includes bibliographical references.

No description available.

No description available.

Protein co-precipitates were prepared from whey powder and soybean flour using various extraction and co-precipitation techniques. The effect of extraction and co-precipitation on co-precipitate yield was investigated. Native and sodium dodecyl sulfate polyacrylamide gel electrophoresis (Native-PAGE, SDS-PAGE) and light compound microscopy (LCM) were used to study the structure of the co-precipitates. The rheological and gelation properties of the co-precipitates were determined. Highest yield (45%) for NaOH/Isoelectric Point IEP-Heating-Cooling, co-precipitate was obtained using the following conditions of extraction; extraction temperature, 40°C; temperature of precipitation 95°C, and pH of precipitation was 4.5. The yield of co-precipitates was affected by chelating agents and pH of precipitation and temperature of precipitation. Native-PAGE showed that 2 new protein bands result from the interactions between whey and soybean proteins during preparation of the co-precipitate. SDS-PAGE showed that the new proteins dissociated to give the protein subunits of whey and soybean proteins. LCM results showed differences in microscopic structure between the whey and soybean protein precipitates and the protein co-precipitates. Gels were characterized by measurement of water holding capacity (WHC), gelation start temperature (GST) and denaturation start temperature (DST) and gel strength (GS). Gels (16%) from a protein co-precipitate Mixed Powder MP:NaOH/IEP-Cooling had higher WHC and GS than gels from whey protein precipitate, soybean protein precipitate and protein co-precipitates Mixed Extract ME:NaOH/IEP-Cooling and co-precipitates MP: and ME:NaOH/IEP-Heating-Cooling. The DST of protein co-precipitates was dependent on protein concentration and pH, while GST was relatively dependent on protein concentration.

No description available.

Call number: LD2668 .T4 1978 K58 / Master of Science

No description available.

This research study was designed to develop new food product formulations from whole cowpeas (Vigna sinensis) with high protein content and specific nutritional characteristics adequate to meet the requirements of both malnourished and well-nourished children. Highly acceptable food products were developed from either spray-dried or dry-roasted flours. These included a chocolate-flavored pudding, cowpea "butter," cookies, frozen desserts and all-purpose, whole wheat/cowpea breads. A rat feeding experiment to evaluate the nutritional qualities of the flours was carried out using Protein Efficiency Ratio (PER) as the evaluated parameter. Weanling male rats were fed diets with 10% protein (unsupplemented and supplemented) from cowpeas or casein (control). Methionine (0.36%), threonine (0.06%) and lysine (0.1%) were the supplementing amino acids used. Unsupplemented diets had very low PERs. The spray-dried flour diet supplemented with methionine had a PER about 15% greater than that of the control. The dry-roasted diet supplemented with methionine, threonine and lysine had a PER approximately equal to the control. It is concluded that it is possible to avoid or alleviate child protein malnutrition with either industrially- or home-produced food products formulated from whole cowpeas.

No description available.

Thesis (M. Sc.)(Biochemistry)--University of Pretoria, 2006. / Includes bibliographical references. Available on the Internet via the World Wide Web.