Binder-based assays are employed throughout the life sciences. Powerful signal amplification techniques have enabled detection of very rare molecule species diluted in simple buffers. Unspecific binding of primary binders leads to increased background in more complex samples. By requiring two recognition events, ligation-based molecular analyses provide highly specific detection of biomolecules in complex samples. We developed a highly multiplexed padlock-ligation assay targeting signature sequences in the hemagglutinin and neuraminidase genes. From a panel of 77 avian influenza isolates of all major serotypes, 97% were genotyped correctly in accordance with previous classifications by classical diagnostic methods (Paper I). Alternative splicing is an important mechanism expanding the proteome. Current analysis techniques fail to provide sequences of complete transcripts beyond the read length of sequencing instruments. We devised and implemented a strategy to compress the sequence information contained in the splicing pattern of a transcript into the presence or absence of sequence-blocks. We demonstrate that this assay yields information about the splicing patterns in thousands of transcripts from cellular cDNA (Paper II). Expression changes of mucin proteins and glycosylation structures are frequently observed from the early stages of cancer development. Expression of mucin 2 and sialyl-Tn are common features of intestinal metaplasia and gastric cancer, and are known to co-locate. Here we have developed an in situ proximity ligation assay (PLA) directed against mucin 2 and sialyl-Tn. Our study on intestinal metaplasia and gastric cancer tissue sections identified mucin 2 as a major carrier of sialyl-Tn in these conditions, and demonstrated how conveniently glycosylation of proteins can be studied by in situ PLA (Paper III). This thesis shows how the dual recognition requirement of ligation-based assays can be employed to detect target molecules with high specificity, to analyze several sequence features of nucleic acids or to study the proximity of two antigens in situ.

This thesis compares the Anglo-Saxon epic Beowulf and John Gardner&amp / #8217 / s novel Grendel in terms of their generic relations within a framework of Bakhtin&amp / #8217 / s genre theory. The analysis restricts its theoretical framework to basically two essays by Mikhail M. Bakhtin, namely Epic and Novel and Discourse in the Novel included in Michael Holquist&amp / #8217 / s The Dialogic Imagination: Four Essays by M. M. Bakhtin (1981). This study argues that Beowulf represents a monologic world, which is hierarchically distanced from the present. As Bakhtin puts it, the epic presents an already completed world placed in an absolute past, which demands a pious attitude as it is hierarchically above the reader. Gardner&amp / #8217 / s Grendel, on the other hand, is a retelling of the Beowulf story through the monster&amp / #8217 / s eye in the contemporary world. It suggests a dialogue between the elevated world of the epic hero Beowulf and the novelistic world of Grendel to achieve multiplicity in a truly Bakhtinian sense. For Gardner&amp / #8217 / s version enables the monstrous other, which is Grendel, to raise its voice. By changing the temporal order and narrative perspective, Gardner succeeds in re-writing an epic story in the novelistic zone of maximal proximity to the present. This thesis, however, argues that although Gardner&amp / #8217 / s Grendel displays all the novelistic features, basically multiplicity and contemporaneity, as put forward by Bakhtin, it still celebrates the ideal world of the epic.

During the past 10 years, construction was the leading industry of occupational fatalities when compared to other goods producing industries in the US. This is partially attributed to ineffective safety management strategies, specifically lack of automated construction equipment and worker monitoring. Currently, worker safety performance is measured and recorded manually, assessed subjectively, and the resulting performance information is infrequently shared among selected or all project stakeholders. Accurate and emerging remote sensing technology provides critical spatio-temporal data that has the potential to automate and advance the safety monitoring of construction processes. This doctoral research focuses on pro-active safety utilizing radio-frequency location tracking (Ultra Wideband) and real-time three-dimensional (3D) immersive data visualization technologies. The objective of the research is to create a model that can automatically analyze the spatio-temporal data of the main construction resources (personnel, materials, and equipment), and automatically measure, assess, and visualize worker's safety performance. The research scope is limited to human-equipment interaction in a complex construction site layout where proximities among construction resources are omnipresent. In order to advance the understanding of human-equipment proximity issues, extensive data has been collected in various field trials and from projects with multiple scales. Computational algorithms developed in this research process the data to provide spatio-temporal information that is crucial for construction activity monitoring and analysis. Results indicate that worker's safety performance of selected activities can be automatically and objectively measured using the developed model. The major contribution of this research is the creation of a proximity hazards assessment model to automatically analyze spatio-temporal data of construction resources, and measure, evaluate, and visualize their safety performance. This research will significantly contribute to transform safety measures in construction industry, as it can determine and communicate automatically safe and unsafe conditions to various project participants located on the field or remotely.

This essay argues that Sara Ahmed’s methodology for reading the emotionality of texts, through its focus on the relationships between emotions, language and bodies, can be applied to the emotional responses of Australians to refugees and asylum seekers. This essay specifically focuses on the emotions of disgust and pain in the participants of Go Back To Where You Came From, a three-part Australian documentary/realia TV series, because these two emotions’ preoccupation with surface and proximity provide a useful metaphor for what can be observed in the participants’ emotionality. Sensuous proximity in the form of sight, taste, smell, touch and hearing underlies the disgust experienced by the Go Back participants, while shared surfaces enable the participants to feel the pain of others. The essay concludes that Ahmed’s methodology is indeed an effective tool for analysing the emotions of people “affected” by the transnational movements of others.

Cylindrically conformal microstrip antennas are investigated. Two different structures, namely proximity coupled and E-shaped microstrip antennas are analyzed and information about the design parameters is obtained by means of parametric study. With these structures, cylindrical arrays, having omnidirectional radiation in the circumferential plane of the cylinder, are designed. Proximity coupled cylindrical arrays operate in the 2.3-2.4 GHz aeronautical telemetry band with approximately 4% bandwidth. On the other hand, more than 30% bandwidth is obtained by E-Shaped cylindrical array antenna structure, which also includes the commercial telemetry band. In order to verify the simulation method, a fabricated antenna in literature is simulated and acceptable agreement with simulation and fabrication results obtained.

Construction has a higher injury and fatality rate than most of the other industries. Given this situation, existing research has studied various issues and factors affecting construction safety management and has attempted to use all available methods to improve the construction safety performance. However, the construction accident rate remains among the highest in the United States and the world. The primary objective of this research is to advance autonomous proximity monitoring and hence provide a safer environment for construction workers. In particular, I seek to advance current evaluations of proximity warning technologies to a more robust engineering approach to the design and deployment of autonomous safety monitoring systems. The contributions of the research are demonstrated through specifications, deployments, and testing of proximity monitoring systems for crane loads and falling from height. My research advances current knowledge in three areas. First, I develop specifications for proximity safety monitoring in a sensed environment, built from existing guidelines and expert interviews. Second, I translate the specifications to computer interpretable rules and deploy them in a distributed computing environment. This demonstrates the feasibility of a systems approach and reusability of components to speed deployment. Third, I evaluate the accuracy of the specifications and systems under imperfect data. I further evaluate some approaches to dealing with imperfect data. Collectively, these advances move existing proximity warning research from evaluation of specific systems to an engineering approach to development and deployment of distributed systems with reusable components that explicitly treats imperfect data. / text

The extent of devastation caused by a biological warfare attack is highly correlated to the time from release to detection. As a step towards lowering the detection time the international project TWOBIAS was launched. Here, the main goal is to develop an automated, specific and sensitive combined detection and identification instrument capable of identifying a biological threat within an hour. The identification unit is comprised of a sample preparation module, an amplification module and a detection module and utilizes a proximity ligation assay in combination with circle-to-circle amplification in order to detect a biological threat. This thesis describes the automation of the sample preparation steps of the assay and the integration with the downstream units. The functionality of the sample preparation module was verified by subjecting it to biological samples in a laboratory and at a real-life location. The results showed that the sample preparation module was capable of preparing a sample collected in a complex environment with the same results as a sample prepared in a laboratory.

The Gaseous state of hydrogen at ambient temperature, combined with the fact that hydrogen is highly flammable, results in the requirement of more robust, high pressure storage systems that can meet modern safety standards. To develop these new safety standards and to properly predict the phenomena of hydrogen dispersion, a better understanding of the resulting flow structures and flammable regions from controlled and uncontrolled releases of hydrogen gas must be achieved. In this study the subsonic release of hydrogen was emulated using helium as a substitute working fluid. A sharp-edged orifice round turbulent jet is used to emulate releases in which leak geometry is circular. Effects of buoyancy, crossflow and adjacent surfaces were studied over a wide range of Froude numbers. The velocity fields of turbulent jets were characterized using particle image velocimetry (PIV). The mean and fluctuation velocity components were well quantified to show the effect of buoyancy due to the density difference between helium and the surrounding air. In the range of Froude numbers investigated, increasing effects of buoyancy were seen to be proportional to the reduction of the Fr number. The obtained results will serve as control reference values for further concentration measurement study and for computational fluid dynamics (CFD) validation. / Graduate

The Electrical Submersible Pump (ESP) manufactured by Baker Hughes, model no. WJE-1000 is designed for wells that are expected to have a high content of abrasive solids. It is a mixed flow, tandem compression type pump. Although the erosion of the pump diffuser and impeller stages are significant, the ESP study shows that the most sever failure is due to components that affect the pump’s rotor dynamics such as radial bearings and impeller seals when eroded with 100 mesh sand. Erosion of these seals will result in an internal leakage that can significantly affect stage pressure rise, efficiency, power consumption, vibration, pump life and running cost. The erosion study utilizing 100 mesh fracture sand at 0.2% concentration, with the pump operating at 3600 RPM, 40 PSI intake pressure, 1150 GPM for over 117 hours comparisons are made to the pump’s baseline performance. Measurements of the rotor bearings, impeller seals and their corresponding stators showed that the wear patterns generally increase with time and differ by location. Stage 1 bearings and seals suffered the least amount of erosion and stage 3 rotor components suffered the most erosion. The maximum change in stage 3 bearing clearances was 223% and the maximum change in stage 3 impeller seal clearances was 300%. Performance wise the total pump efficiency dropped by 6.77%, the total pressure rise dropped by 6.3%, the pump’s best efficiency point decreased by 0.78%, and the power consumption increased by 0.49%. Pump vibration patterns also changed with time and by location. The maximum shaft orbit diameter was at stage 3 and it grew 643% in diameter after 117 hours of erosion. The waterfall plots of the pump’s ramp up changed significantly with time. After 117 hours at 3600 RPM, sub-synchronous oscillations at 67% of the synchronous speed dominated the amplitude peaks showing that the rotor vibration locked with the rotor’s first natural frequency at around 2500 RPM. After 117 hours, another sub-synchronous started showing a peak at the rotor’s second natural frequency at 1500 RPM.

This study seeks to make a theoretical contribution to the rapidly growing field of International Entrepreneurship by investigating the process of internationalization of Small and Medium Enterprises (SMEs). Bell, McNaughton, Young & Crick, (2003) emphasized the need for researchers to re-conceptualize their thinking on the internationalization process of smaller firms. While there has been substantial research done on the small business internationalization and how the participation of these firms in the global economy has fuelled economic growth in a number of countries (Audretsch & Thurik, 2003; Acs, Randall Morck, Shraver & Yeung, 1997; Storey, 1994; Alam & Pacher, 2003), there is tangible evidence that SMEs in Australia are not keeping up with global trends. Studies conducted on Australian firms allude to the following reasons for their constrained presence in international markets: (i) geographic and psychic distance; (ii) costs disadvantage; (iii) overdependence on inward FDI from large foreign multinationals; (iv) a history of inward-looking and narrowly focussed economic development policies of the Australian government. These factors had the combined effect of imbalanced economic growth which was particularly detrimental to the small business sector (Australian Trade Commission, 2002; Maitland & Nicholas, 2002; Alam & Pacher, 2003). Despite the identified obstacles, some Australian SMEs have succeeded in penetrating international markets. This study involved a preliminary qualitative investigation of selected Australian SMEs and their unique internationalization process. Evidence from the case study based investigation will indicate that the respondent firms have leveraged on entrepreneurial qualities to overcome the obstacles and enhance their success in international markets. As such, the internationalization process for these selected firms is seen as an extension of and integral to their entrepreneurial behaviour. Using the lenses provided by relevant facets of the entrepreneurship, internationalization and strategy scholarly fields, this exploratory qualitative study, while building the foundation for further empirical research into the internationalization process of SMEs, can serve as a guide to researchers for ascertaining future directions in this emergent field. The findings from the study are intended to contribute to a body of knowledge encompassing the cross-border operations of SMEs. The research also has value from a practical perspective as Australian SMEs can draw from this body of knowledge as they pursue opportunities internationally.