Thesis (M. Sc. (Botany)) -- University of the North, 1986 / Refer to the document

Pterocarpus angolensis (kiaat) is one of 20 species of trees in the pantropical genus Pterocarpus. The species occurs in Southern Africa and has a broad distribution over several African countries, including Tanzania, Democratic Republic of Congo, Angola, Zambia, Mozambique, Namibia, Botswana, Zimbabwe and South Africa. The tree is commercially used in several traditional medicinal applications and is well-known as a source of timber for woodcarving and furniture. A single serious disease known as mukwa has been reported on P. angolensis, and the cause has been attributed to the generalist fungus Fusarium oxysporum. Reports of this disease date back to the late 1950s and appear localized in the Livingstone distinct, between Livingstone in Zambia and Bulawayo in Zimbabwe. Recent studies indicate that mukwa disease has begun to subside. There are more recent reports, however, of P. angolensis trees dying in South Africa, in the Mpumalanga Province. The aim of the research presented in this dissertation was to establish the nature of the disease affecting P. angolensis trees in the Mpumalanga Province in South Africa. Trees were sampled from several areas in the province, both from reported disease areas and from areas sustaining healthy trees. Field observations and fungal isolations provided a means to determine whether the disease was attributable to a biotic factor or the result of environmental factors affecting the tree. Isolations concentrated on obtaining species of the Botryosphaeriaceae and of the so-called ophiostomatoid fungi because these fungi are well-known pathogens of trees in the area, have been fairly well studied and the die-back symptoms suggested that they could be involved. The first chapter of this dissertation presents a review of the literature pertaining to P. angolensis, with a focus on mukwa disease. Aspects of the review introduce the genus Pterocarpus, the ecology of the environment wherein P. angolensis occurs and cover the biology of the species. Commercial aspects of the tree as well as the need for conservation are also addressed, providing the foundation for further studies in this dissertation. Many of the symptoms of diseased trees studied were indicative of infection by the Botryosphaeriaceae, a group of several genera of endophytic plant pathogens. These fungi were thus isolated from P. angolensis trees growing in different areas and were subsequently identified. Pathogenicity tests were used to evaluate the possible role that these species might play in the dieback of P. angolensis. Copyright / Dissertation (MSc)--University of Pretoria, 2010. / Microbiology and Plant Pathology / unrestricted

Thesis (M.Phil. (Environmental Law)) --University of Limpopo, 2008 / National Research Foundation, and the SANPAD scholarship

Thesis (MSc)--Stellenbosch University, 2015. / ENGLISH ABSTRACT: Pterocarpus angolensis is one of the timber tree species that regenerates naturally in the dry forest of Namibia, mainly assisted by the influence of forest fires. Tree development goes through a prolonged suffrutex stage to reach the sapling stage and then, finally, the bole tree stage. This study focused on assessing the main factors facilitating the development of Pterocarpus angolensis seedlings through the suffrutex stage to the sapling stage in Namibia dry forests. To achieve the study objectives three study locations (Okongo and Ncumcara Community Forests and Caprivi State Forest) were selected, representing a rainfall gradient. Within each study location, two different fire history treatments (recently burnt (RB) and recently unburnt (RU)) were selected, and four plots were randomly selected from each fire history treatment. Face to face individual interviews was conducted with community members surrounding the three forests to obtain indigenous knowledge information about Pterocarpus angolensis tree development. Seedlings and saplings found in all plots were counted and measured (tree height and diameter at breast height (DBH)) while trees more than 3 m high were only counted and measured for DBH. Laboratory analysis was performed to determine basic soil texture and nutrient status. In addition, destructive sampling was done on individual trees in the seedling and sapling stages at each study location. The destructive samples allowed for estimation of biomass in above and below ground components, determination of carbohydrate storage in the taproots and estimation of tree age by counting growth rings on the neck disc of the taproot sample. These measures could shed light on the tree development through the suffrutex stage. The main agents causing Pterocarpus angolensis tree damage and stand disturbances observed are drought, fires, insects, diseases, temperature, lightning, wind, animals and humans. Forest fires were found to be one of the major disturbances in all the study locations, particularly damaging to seedlings when fire intensity is high. Likewise, the most important factors influencing the tree development from seedlings to sapling and sapling to bole tree stages are soil water, soil fertility, plant competition, sunlight and fires. Through counting growth rings of taproot neck discs, it is estimated that the ages of seedlings most commonly range from 5 to 12 years in the dry forests of Okongo, Ncumcara and Caprivi. The soil texture in the three forests is dominated by sand, with the soil reaction usually being moderately acidic while the soils have low levels of organic carbon, phosphorus and exchangeable base cations. This study revealed that Caprivi State Forest (location with the highest rainfall) has the highest stand density followed by Okongo Community Forest and Ncumcara Community Forest with the lowest. Trees were grouped into different DBH and height classes. The highest numbers of trees are found in DBH class 0 – 10 cm and in height class 0.6 – 1.0 m at Okongo Community Forest but at Ncumcara and Caprivi many of the trees are in height class 1.1 – 1.5 m. The mean DBH difference is significant between locations but not significant between fire history treatments. A higher abundance of mature trees are found at Okongo Community Forest while a greater abundance of saplings occur at Ncumcara Community Forest which shows a significant difference between study locations. Seedling abundance is the same across study locations and fire history treatments. The difference in stand structure between study locations appears to be strongly influenced by different management regimes on the three locations. A majority of respondents from all the study locations alleged soil water followed by soil fertility as the main influential factors to Pterocarpus angolensis development. Again, most of the respondents revealed that seedling takes 4 – 7 years to reach sapling stage and their main environmental disturbance is fire. Tree cutting by members of the community was also perceived by the respondents as an important non-environmental disturbance. The most abundant tree development stage perceived by respondents was mature trees while seedlings rated the sparsest stage. Based on the respondents no silvicultural practices are performed to promote Pterocarpus angolensis growth. It follows that the Kiaat trees are currently growing without human intervention that might enhance their development. A combination of social survey (interview) and ecological survey provided reliable information on ecological processes. A weak positive significant correlation relationship existed between shoot mass (aboveground biomass) and taproot mass (belowground biomass), meaning when the taproot mass increases the shoot mass also increases. Analysis of non-structural carbohydrates (NSC) storage in taproots showed that both sugar and starch contents in the taproots could facilitate the survival of the tree during suffrutex stages and its rapid growth thereafter. Based on this study Pterocarpus angolensis regeneration in these three dry forests is poor because seedling abundance is the lowest compared to saplings and mature trees. These study findings can be used as the basis for further studies to predict Pterocarpus angolensis natural regeneration in the dry forests, as well as input when management regimes are being developed for the dry forests of North Namibia. / AFRIKAANSE OPSOMMING: Pterocarpus angolensis (Kiaat) is een van die boomspesies wat natuurlik verjong in die droë bosveld van Namibië, met die hulp van bosbrande. Die boom ontwikkel deur ʼn lang semi-struik stadium waartydens die boompies as saailinge bekendstaan. Daarna ontwikkel dit deur die jongboom stadium tot dit uiteindelik die kroon stadium bereik. Hierdie studie fokus op die faktore bydra tot die ontwikkeling van Pterocarpus angolensis van die semi-struik stadium na die jongboom stadium in die droë bosveld van Namibië. Om die doelstellings van die tesis te bereik is drie studiegebiede gekies langs ʼn reënvalgradiënt (naamlik Okongo en Ncumcara gemeenskapsbosse asook Caprivi Staatsbos). Binne elke studiegebied is twee behandelings met verskillende brandgeskiedenis gekies (gebrand of nie-gebrand in die onlangse verlede). Vier persele is ewekansig uit elk van hierdie behandelings gekies vir eksperimentering. Persoonlike onderhoude is gevoer met gemeenskapslede wat in die omgewing woon ten einde inheemse kennis en inligting te versamel oor die ontwikkeling van die jong Pterocarpus angolensis bome. Alle saailinge en jongbome wat voorkom in die persele is getel en gemeet (boomhoogte en deursnee op borshoogte (DBH)) terwyl bome wat hoër as 3 m is, slegs getel en vir DBH gemeet is. Laboratoriumtoetse is gedoen op grondmonsters ten einde ‘n basiese beskrywing van die grondtekstuur en voedingstofstatus te verkry. Verder is destruktiewe bemonstering toegepas op bome in beide die saailing en jongboom stadium op elke studiegebied. Hierdie bemonstering het dit moontlik gemaak om bogrondse en ondergrondse biomassa te skat, om die opberging van koolhidrate in die penwortels te bepaal, en ook om die boom ouderdom te skat vanaf jaarringe in die nek van die penwortel monster. Hierdie metings kon lig werp op die boomontwikkeling deur die semi-struik stadium. Die faktore wat skade aan Pterocarpus angolensis bome veroorsaak asook versteuring van die opstande waarin die bome voorkom is droogte, brande, insekte, siektes, temperatuur uiterstes, weerlig, wind, diere en mense. Die bevindinge dui op bosbrande as een van die belangrikste versteuringsfaktor in al drie studiegebiede; dit is veral skadelik vir saailinge in die semi-struik stadium wanneer die vuurintensiteit hoog is. Die faktore wat die boomontwikkeling van saailing, na jongboom en kroonstadium beïnvloed is hoofsaaklik grondwater, grondvrugbaarheid, plantkompetisie, sonlig en brande. Die ouderdom van saailinge (bepaal vanaf jaarring tellings in die nek van penwortel monsters) van die meeste saailinge én jongbome is na raming tussen 5 en 12 jaar vir die droë bosse in die studiegebiede van Okongo, Ncumcara en Caprivi. Die grondtekstuur van hierdie studie se drie bosgebiede is hoofsaaklik sanderig, met ’n effens suur grondreaksie terwyl die gronde lae vlakke van organiese koolstof, fosfor, en uitruilbare basiese katione bevat. Die studie het aangedui dat Caprivi staatsbos (met die hoogste reënval) die digste opstande huisves, gevolg deur Okongo en dan Ncumcara gemeenskapsbos, met die laagste digtheid. Bome is gegroepeer in verskillende DBH en hoogte klasse. Die meeste bome kom voor in die DBH klas van 0-10 cm en in die hoogteklas van 0.6 – 1.0 m by Okongo, maar by Ncumcara en Caprivi is daar meer bome in die hoogteklas van 1.1 - 1.5 m. Die gemiddelde DBH verskil is betekenisvol tussen studiegebiede, maar is nie betekenisvol verskillend tussen brandgeskiedenis behandelings nie. ’n Hoër voorkoms van volwasse bome is by Okongo aangetref, terwyl ’n hoër voorkoms van jongbome by Ncumcara waargeneem is, en hierdie verskil was statisties betekenisvol. Die voorkoms van saailinge is soortgelyk oor alle studiegebiede en brandgeskiedenis behandelings heen. Die verskil in die struktuur van die opstande op die drie studiegebiede word skynbaar sterk beïnvloed deur verskillende bestuurspraktyke wat in elke gebied toegepas word. Die meerderheid van respondente van al drie studiegebiede beweer dat grondwater, gevolg deur grondvrugbaarheid die belangrikste faktore is wat P. angolensis ontwikkeling beïnvloed. Meeste van die respondente onthul dat saailinge 4 tot 7 jaar neem om die jongboom stadium te bereik en dat die belangrikste versteuringsagent bosbrande is. ʼn Belangrike nie-omgewingsfaktor wat verantwoordelik is vir versteuring in die bosse is mense wat bome, lote en/of takke afsaag. Respondente is van mening dat volwasse bome die grootteklas met die mees algemene voorkoms is, terwyl saailinge die skaarsste grootteklas uitmaak. Die respondente het aangedui dat geen boskultuurpraktyke toegepas word om die groei van P. angolensis aan te help nie. Die gevolgtrekking is dus dat die Kiaatbome tans groei sonder menslike ingryping om hul ontwikkeling te verbeter. Die kombinasie van persoonlike onderhoude en ʼn ekologiese opnames het betroubare inligting rakende ekologiese prosesse opgelewer. ’n Swak positiewe, maar betekenisvolle korrelasie bestaan tussen die massa van die bogrondse lote en die penwortelmassa, wat beteken dat die lote se massa toeneem met toenemende wortelmassa. Analise van opgebergde nie-strukturele koolhidraatreserwes in die penwortel toon dat beide suiker- én styselinhoud in die penwortels die oorlewing van die boom in die struikstadium aanhelp, asook sy vinnige groei na die struikstadium. Die feit dat die saailinge minder volop is as jongbome en volwasse bome in hierdie studie dui aan dat verjonging van Pterocarpus angolensis in hierdie droë bosse maar swak is. Die bevindinge van die studie bevat inligting wat gebruik kans word (a) as die grondslag van verdere studies op die natuurlike verjonging van Pterocarpus angolensis in droë bosse, en (b) as inset wanneer bestuursaanbevelings vir die droë bosse van Noord Namibië ontwikkel word.

Thesis (M.Sc. (Microbiology)) --University of Limpopo, 2011 / Pterocarpus angolensis seed lectin (PAL), a 28 kDa non glycosylated protein, was initially successfully cloned and expressed in E. coli for ease of high protein production. It was discovered, however, as in similar studies that the recombinant PAL yield in E. coli is low and localized intracellularly. This makes extraction even more difficult because most of the protein is lost either when the cell undergoes lysing or when there is incomplete extraction. As a result of the low yields in E. coli, expression vectors were constructed for pal expression in S. cerevisiae, Y. lipolytica and A. niger. Colony PCR of S. cerevisiae transformants confirmed the presence of pal gene whilst sequencing revealed a 66% homology to native PAL. Expression of recombinant PAL in S. cerevisiae, which was expected to be intracellular, was doubtfully unsuccessful since no signal was detected following Western blot analysis. A pBARMTE1-pal expression vector was successfully constructed and could be used for expression studies in Aspergillus niger, however, it was not used in this study. A pal gene whose codons were optimized for Y. lipolytica was synthesized and successfully cloned and expressed in Y. lipolytica. Gene sequence alignment of native pal and the codon optimized pal showed 81% homology whilst the amino acid alignment showed 100% homology. A 31 kDa, recombinant PAL was successfully expressed in Y. lipolytica. The recombinant PAL was approximately 3 kDa larger than native PAL. It was established that this is due to glycosylation of the recombinant PAL. This recombinant protein was found to be more thermostable than native PAL since it demonstrated haemagglutination activity after 10 minutes of exposure in a boiling water bath and only lost activity after 2 hours of exposure to boiling. This study succeeded in producing a more stable extracellular recombinant PAL which demonstrated biochemical activity that was largely similar to that of native PAL but only differed in carbohydrate specificity and haemagglutinating strengths. / Flemish Interuniversity Council (VLIR-UOS)-Own Initiative Project,the SARBIO- South African Regional Co-operation in Biochemistry, Molecular Biology and Biotechnology, the (CSIR) Council for Scientific and Industrial Research,the (NRF) National Research Foundation,(TBI) The Biovac Institute Foundation, and the (SIDA) Swedish International Agency

Thesis (MScFor)--Stellenbosch University, 2013. / ENGLISH ABSTRACT: Kiaat trees in Namibia are threatened by unsustainable harvesting practice. This study’s aim was to estimate the total value of an average Kiaat tree selected for harvesting with a focus on current timber use value, value of an optimally utilised tree, carbon value, and alternative uses of Kiaat trees. To accomplish this aim, the following specific objectives were set: (1) to estimate the total tree volume and optimum utilisable timber volume of an average Kiaat tree; (2) to estimate the amount and value of carbon stored in the above-ground parts of an average Kiaat tree; (3) to determine timber utilisation levels; and (4) to identify alternative use options to reduce timber losses of Kiaat trees. Previous studies on Kiaat in Namibia and elsewhere focused on growth, development and socio-economical aspects of Kiaat trees. A combination of a socio-economic survey and tree volumes and biomass determination was used to collect data. The survey entailed Kiaat products assessment and face-to-face personal interviews with known key-informants in and around Rundu. Loggers with permits to harvest Kiaat trees were asked to harvest 40 Kiaat trees and data collected from each tree before and after felling included: lower-stem diameter, diameter at breast height (DBH), upper-stem diameter, stem length, stump height, recovered merchantable logs lengths and lower-and-upper diameters and lengths of branches greater than 10 cm in diameter. All canopy parts smaller than 10 cm in diameter were directly weighed, sampled and oven-dried at 105°C until constant weight. Volume of different tree parts was calculated and in combination with basic wood density used to calculate the biomass. It was established that a typically harvested Kiaat tree had an above-ground dry volume of 1.63 m3, of which 1.34 m3 (82%) was utilisable timber volume but that only 0.37 m3 (23%) was used and 0.97 m3 (59%) was left behind in the field. Merchantable logs were mainly cut into planks from which finished products - beds, chairs, doors and tables - were made. An average of 10.7 planks were cut per trunk and the local price of planks was N$45.26 at the time of the study. More income is generated from finished products compared to selling loose planks. Canopy parts were mainly cut into woodcrafts – bowls, music drums, and walking sticks. Current timber use value (N$484.73) surpasses carbon value (N$123.74). A further result of the study was that a significantly higher income could be earned for local livelihoods from Kiaat trees in the Kavango Region if trees were optimally used. Carbon trading is a noble conservation initiative, particularly when trees unsuitable for timber are considered. Use of timber trees exclusively for carbon trading is, however, not a viable option in respect of supporting local people’s livelihoods. / AFRIKAANSE OPSOMMING: Kiaatbome in Namibië word bedreig deur onvolhoubare ontginningspraktyke. Die doelwit van die studie was om die totale waarde van ‘n gemiddelde Kiaatboom, wat gekies is vir ontginning, te bepaal met die fokus op huidige houtwaarde, waarde van ‘n optimaal gebruikte boom, koolstofwaarde en alternatiewe gebruike vir Kiaatbome. Om hierdie doelwit te bereik is die volgende spesifieke sub-doelwitte gestel: (1) bepaal die totale boomvolume en optimale bruikbare houtvolume van ‘n gemiddelde Kiaatboom; (2) bepaal die hoeveelheid koolstof wat in die bogrondse dele van ‘n gemiddelde Kiaatboom gestoor word en bereken die monitêrewaarde daarvan; (3) bepaal die houtgebruiksvlakke en houtvermorsing van Kiaatbome; en (4) identifiseer alternatiewe gebruike om die vermorsing van Kiaatbome te verminder. Vorige studies oor Kiaat in Namibië en elders het gefokus op groei, ontwikkeling en sosio-ekonomiese aspekte van Kiaatbome. ‘n Kombinasie van ‘n sosio-ekonomiese opname en boomvolume en biomassa bepalings is gebruik vir die insameling van data. Die opname het ‘n Kiaat produkbepaling en gesig-tot-gesig persoonlike onderhoude met sleutel informante in en om Rundu behels. Boomkappers met permitte om kiaatbome te ontgin is gevra om 40 Kiaatbome te ontgin en data is versamel van elke boom voor en na ontginning. Die data het ingesluit: lae-stam deursnee, deursnee op borshoogte (DBH), bo-stam deursnee, stamlengte, stomphoogte, herwinde bruikbare stomplengte, bo- en onder- deursnee en lengtes van takke wat 10 cm of groter deursnee het. All kroondele 10 cm en kleiner in deursnee is geweeg en ‘n steekproef versamel wat oondroog gemaak is by 105ºC totdat konstante gewig bereik is. Die volume van verskillende boomdele is bereken en in kombinasie met houtdigtheid gebruik om biomassa te bereken. Dit is bereken dat ‘n tipiese geoeste Kiaatboom ‘n bogrondse droë volume van 1.63 m³ het, waarvan 1.34 m³ (82%) bruikbare houtvolume is. Net 0.37 m³ (23%) van die bruikbare houtvolume is egter gebruik en 0.97 m³ (59%) is agtergelaat in die veld. Bruikbare stompe is meestal opgesaag in planke waarvan finale produkte soos beddens, stoele, deure en tafels gemaak is. ‘n Gemiddeld van 10.7 planke is verkry per stomp en die plaaslike prys van planke was N$45.26 gedurende die studie tydperk. Meer inkomste is verkry van finale produkte as van die verkoop van los planke. Kroondele is meestal opgesaag in houtkunswerke soos bakke, musiekdromme en kieries. Die huidige houtwaarde van N$484.73 is meer as die koolstof waarde (N$123.74) van die bome. ‘n Belangrike resultaat van die studie is dat ‘n beduidende groter inkomste gegenereer kan word vir plaaslike lewensbestaan van Kiaatbome in die Kavangostreek as bome optimaal benut word. Koolstofhandel is ‘n edele bewaringskonsep, veral as bome ongeskik vir houtgebruik is soos in die geval van krom bome en jonger bome. Die eksklusiewe gebruik van houtbome vir koolstofhandel is nie ‘n lewensvatbare opsie om plaaslike mense se lewensbestaan te ondersteun nie.