"Submitted to the Faculty of the Graduate School of the University of Wisconsin in partial fulfillment of the requirements for the degree of Doctor of Philosophy" (1932). / Reprinted from Scientific agriculture, vol. XIV, no. 4 (Dec. 1933). Includes bibliographical references (p. 235-237).

Thesis (Ph. D.)--University of Minnesota, 1924. / Cover title. Vita. "Reprinted from Phytopathology, January, 1928, vol. xviii, no. 1." Bibliography: p. 121-123.

Thesis (Ph. D.)--University of Minnesota, 1924. / Published also as University of Minnesota Agricultural Experiment Station, Technical bulletin 35, December, 1925. "Literature cited": p. 32-33.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1957. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 80-88).

Thesis (Ph. D.)--University of Wisconsin--Madison, 1953. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 41-42).

Thesis (Ph. D.)--Washington State University, May 2009. / Title from PDF title page (viewed on June 1, 2009). "Department of Crop and Soil Sciences." Includes bibliographical references (p. 34-43).

Thesis (Ph. D.)--Oregon State University, 2007. / Printout. Includes bibliographical references (leaves 93-99). Also available on the World Wide Web.

Zugl.: Giessen, Univ., Diss., 2010

The aim of this work is the accurate diagnosis of the Puccinia species of the selected turf grass species under field and laboratory conditions and evaluate influences on the intensity of occurrence. The laboratory tests will be recommended for the best stage of plants to artificial infection. The experiment was carried out in collaboration with SŠ Větrov. In the two-year follow-up (in 2011 and 2012) were at particular times of samples of infected plants, which were then evaluated microscopically. And at the same time experimental work was carried out in laboratory conditions (artificial infection) in plant material of the same origin and assessed degree assault with a precise diagnosis of the type of pathogen. Between 2011 and 2012, was higher occurence stem rust than crown rust.. From a total of 514 tests was stem rust in 379 cases, while crown rust in only 40 cases. In 95 samples was observed incidence of both simultaneously rust and mildew spores remaining plants were discovered. Greenhouse resistance tests were performed on younger plants (3 sheets) and older (6 sheets) in 16 variants, of which 4 were control. Infected leaves were spray uredispor with talc and injected with an aqueous suspension followed by looking drops and were then sealed under insulators at 23 ° C for 24 - 48 hours.

Germinating uredospores of race C17 of Puccinia graminis f.sp. tritici form characteristic infection structures (appressorium, infection peg, vesicle, infection hypha) in response to a 1.5 h heat shock at 29° C administered 2 h after germination at 19° C. The proportion of sporelings forming infection structures was augmented by nutrients, n-nonyl alcohol and, an appropriately timed heat shock. The heat shock temperature required to induce maximum differentiation had a very precise optimum which varied slightly for each spore lot. Variations one degree above or below this optimum reduced the percent differentiation by greater than 40%. The presence of an inhibitor of protein synthesis, puromycin, in the germination medium: (1) prevented uredosporeling differentiation but had no effect on germination, (2) significantly reduced the proportion of germtubes forming appressoria, and (3) in most cases prevented the division of germtube nuclei. It was concluded that essential differentiation-specific proteins are synthesized from the onset of germination, throughout the formation of appressoria and to the completion of differentiation. These results were consistent with the observed effects of heat shock on the rate of protein hydrolysis. During germination there was a net hydrolysis of protein leading to an increase in size of the endogenous pool of free amino acids and to an increased leakage of amino acids to the germination medium. Heat shock effectively reduced the amount of endogenous free amino acids and the extent to which amino acids were lost to the medium. It was concluded that in heat shocked sporelings protein synthesis was increased relative to protein hydrolysis by comparison with the relative rates of these two processes in germinating (non-shocked) uredosporelings. Moreover, there was no net protein synthesis during the formation of infection structures induced by heat shock. The loss of amino acids to the germination medium was selective, particularly in heat shocked sporelings. / Land and Food Systems, Faculty of / Graduate