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Thesis (Ph. D.)--Chemistry and Biochemistry, Georgia Institute of Technology, 2009. / Committee Chair: Hud, Nicholas; Committee Member: Doyle, Donald; Committee Member: Lobachev, Kirill; Committee Member: Oyelere, Adegboyega; Committee Member: Wartell, Roger. Part of the SMARTech Electronic Thesis and Dissertation Collection.

Leishmania parasites are reliant on salvage mechanisms to acquire purines from the extracellular environment. GMP reductase (GMPR) catalyzes the conversion of GMP to IMP, an integral reaction for maintaining purine nucleotide balance. Enzymatically active L. major GMPR (LmGMPR) has been cloned, expressed and purified. The LmGMPR gene complements GMPR deficiency in E. coli strains. Quaternary structure analysis indicates that LmGMPR forms tetramers and higher order complexes under reducing conditions. Kinetic assays reveal that the enzyme deviates from hyperbolic behaviour with regard to GMP but conforms to typical Michaelis-Menten kinetics for NADPH. Sequence analysis indicates that LmGMPR contains CBS domains and an MPA binding site. MPA competes for the NADPH binding site with a K i of 20 muM. ATP and GTP regulate enzymatic activity through inhibition and activation, respectively. This data indicates that LmGMPR is a novel enzyme that performs a highly regulated step in Leishmania purine metabolism.

Thesis (Ph.D.) -- Flinders University, Dept. of General and Digestive Surgery, School of Medicine. / Typescript (bound). Includes bibliographical references (leaves 254 - 270). Also available online.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1959. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 57-62).

Thesis (M.S.)--University of Missouri-Columbia, 2008. / The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file (viewed on August 14, 2009) Includes bibliographical references.

Thesis (Ph. D.)--Michigan State University. Neuroscience, 2008. / Title from PDF t.p. (Proquest, viewed on Aug. 17, 2009) Includes bibliographical references (p. 202-230). Also issued in print.