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Effects of N1-methylated purines on DNA double helical structures and stabilities.January 2007 (has links)
Zhan, Yingqian. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2007. / Includes bibliographical references (leaves 49-54). / Abstracts in English and Chinese. / Title Page --- p.i / Thesis Committee --- p.ii / Abstract (English version) --- p.iv / Abstract (Chinese version) --- p.v / Acknowledgment --- p.vi / Table of Contents --- p.vii / List of Tables --- p.ix / List of Figures --- p.x / List of Abbreviations and Symbols --- p.xiii / Chapter 1. --- Introduction --- p.1 / Chapter 1.1 --- DNA Methylation --- p.1 / Chapter 1.2 --- DNA Structure --- p.2 / Chapter 1.2.1 --- Nomenclature Scheme for DNA --- p.2 / Chapter 1.2.2 --- Conformations of DNA --- p.5 / Chapter 1.2.3 --- Base Pair Scheme --- p.5 / Chapter 1.2.4 --- Sugar Conformation --- p.7 / Chapter 1.2.5 --- Backbone Conformation --- p.7 / Chapter 1.3 --- DNA Melting --- p.7 / Chapter 1.4 --- Objective of this Work --- p.8 / Chapter 2. --- Materials and Methodology --- p.9 / Chapter 2.1 --- Sequence Design --- p.9 / Chapter 2.2 --- Sample Preparation --- p.10 / Chapter 2.3 --- Resonance Assignment --- p.10 / Chapter 2.3.1 --- Proton Resonance Assignment --- p.11 / Chapter 2.3.2 --- Phosphorous Resonance Assignment --- p.14 / Chapter 2.4 --- Determination of Sugar Conformation --- p.15 / Chapter 2.5 --- Determination of Backbone Conformation --- p.16 / Chapter 2.6 --- Thermodynamic study on DNA --- p.18 / Chapter 3. --- Effects of N1-methylated Adenine on DNA Structure and Stability --- p.21 / Chapter 3.1 --- Overview --- p.21 / Chapter 3.2 --- Resonance Assignment Results --- p.21 / Chapter 3.2.1 --- 1H Resonance Assignments --- p.21 / Chapter 3.2.2 --- 31P Resonance Assignments --- p.23 / Chapter 3.3 --- Base Pair Structures --- p.24 / Chapter 3.4 --- Sugar Conformation --- p.26 / Chapter 3.5 --- Backbone Conformation --- p.27 / Chapter 3.6 --- Thermodynamic Stability Study --- p.29 / Chapter 4. --- Effects of N1-methylated Guanine on DNA Structure and Stability --- p.31 / Chapter 4.1 --- Overview --- p.31 / Chapter 4.2 --- Resonance Assignment Results --- p.31 / Chapter 4.2.1 --- 1H Resonance Assignments --- p.31 / Chapter 4.2.2 --- 31P Resonance Assignments --- p.33 / Chapter 4.3 --- Base Pair Structures --- p.34 / Chapter 4.4 --- Sugar Conformation --- p.36 / Chapter 4.5 --- Backbone Conformation --- p.39 / Chapter 4.6 --- Thermodynamic Stability Study --- p.41 / Chapter 5. --- Conclusion and Future work --- p.43 / Appendix I H1'-H6/H8 region of NOESY spectra of TmeA at 25 °C with different mixing times --- p.44 / "Appendix II 3JH1'h2"", 3JH1'h2"", and %S of TmeA and refTA" --- p.45 / "Appendix III 3JH1' h2',3JH1'-H2"", and %S of CmeG and refCG" --- p.46 / "Appendix IV 31P chemical shifts, 3JH3'p and %B1 of TmeA, and refTA" --- p.47 / "Appendix V 31P chemical shifts, 3JH3'P and %B1of CmeG, and refCG" --- p.48 / References --- p.49
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