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Optofluidic Detection of Aqueous Ammonia and Parasitic CystsTemkov, Melissa January 2022 (has links)
Water quality monitoring in Canada is essential to providing safe water to all. Indigenous and remote communities, many of which are under boiling drinking water advisories, lack availability and/or funding to water monitoring resources. A low-cost, point-of-care detection mechanism has been proposed for the detection of aqueous ammonia and protozoan parasites, which affect the safety of a source of water. An ammonia fluorescence responsive hydrogel, based on the fluorescence quenching of rare earth metal Europium (Eu3+) upon contact with aqueous ammonia, has been proposed to be incorporated into a microfluidic device, which utilizes shadow imaging and flow analysis to detect parasitic (oo)cysts of Cryptosporidium and Giardia, two of the most prevalent protozoan parasites which cause gastrointestinal illness around the world. Fabrication of the ammonia sensitive hydrogel was completed, and the essential components to the ammonia sensitivity were determined. Chemical analysis and solvent modifications found that Formamide is the essential solvent to maintain ammonia sensitivity. A literature review into the current detection mechanisms of Cryptosporidium and Giardia was completed to provide a reference and starting point for the development of the low-cost, point-of-care device proposed in this thesis. Baseline images of Cryptosporidium parvum and Giardia lamblia were captured to provide a reference for the development of a particle tracking algorithm to be used in the microfluidic device. The images captured highlight morphological features essential to developing a tracking mechanism based on the morphology of the (oo)cysts. / Thesis / Master of Science in Biomedical Engineering / Water quality monitoring for remote and Indigenous communities is needed to provide safe water to all. Detection of aqueous ammonia by fluorescent hydrogel, and parasitic cysts by flow analysis, provides a low-cost, point-of-care detection mechanism. A fluorescence responsive hydrogel for aqueous ammonia detection was produced and the essential components for ammonia responsiveness were determined. Detection mechanisms of parasites Cryptosporidium and Giardia, two of the most prevalent parasitic protozoans causing human gastrointestinal illness, were analyzed and compared. Baseline images of the parasitic (oo)cysts were captured by conventional microscopy for the training of particle tracking algorithms to be implemented into a microfluidic device. The microfluidic device detection mechanism will utilize shadow imaging and flow analysis to detect parasitic (oo)cysts.
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Chitobiase as a tool in water quality monitoringMacKenzie, Scott 11 March 2016 (has links)
Time-consuming and expensive benthic surveys are currently the most common means of determining impacts on invertebrates and fish habitat in lotic systems. We propose using the rate of chitobiase production by benthic invertebrates as a complement for determining impacts on freshwater systems. We successfully modified the existing assay to a microplate approach for high throughput analysis of chitobiase activity. Next we conducted two case studies in: 1) the Dead Horse Creek, Manitoba, to determine if changes in chitobiase could detect impacts on the benthic community from wastewater effluent and; 2) in Snake and Kinch Creeks, Manitoba to see if chitobiase could be used to assess fish habitat quality. In both cases, we observed no strong relationships between chitobiase and traditional metrics (e.g., abundance, biomass, diversity). We recommend further studies concerning the timing of chitobiase release in lotic systems and assessments of its use in mesocosm and microcosm toxicity studies / May 2016
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The Design of a DSP Based Power Quality Monitoring DeviceLin, Jin-Yi 03 July 2001 (has links)
Electric power utilities and end users are becoming increasingly concerned about the quality of power supply. To reduce the losses caused by power service disturbances, mitigation devices are available for improving the power quality. The first step in the power quality improvement is to monitor the system behavior by using some Electronic recording devices. A design and implementation of a digital signal processor based power quality monitoring device is presented in this thesis. Several event-triggering methods are studied and implemented to detect system disturbances. Simulation and test results indicate that the proposed design can meet the requirements for power measurements and transient event recording during steady and transient states.
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A critical review of Hong Kong air quality data /Ip, To-yan, Francis. January 2001 (has links)
Thesis (M. Sc.)--University of Hong Kong, 2001. / Includes bibliographical references (p. [84-85]).
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A mathematical procedure for air monitoring instrumentation locationLee, Thomas Davis 08 1900 (has links)
No description available.
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Study of air-borne polycyclic aromatic hydrocarbons in El Paso, TXSantiago, Lynn Marie, January 2008 (has links)
Thesis (M.S.)--University of Texas at El Paso, 2008. / Title from title screen. Vita. CD-ROM. Includes bibliographical references. Also available online.
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Application of cluster analysis to identify sources of particulate matter in Hong Kong /Chan, Sik-foon, Joyce. January 1995 (has links)
Thesis (M. Sc.)--University of Hong Kong, 1995. / Includes bibliographical references.
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Chance constrained models for air pollution monitoring and control /Lelas, Vedran, January 1998 (has links)
Thesis (Ph. D.)--University of Texas at Austin, 1998. / Vita. Includes bibliographical references (leaves 226-244). Available also in a digital version from Dissertation Abstracts.
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Participatory Air Quality Monitoring SystemChoi, Daeyoung 08 September 2010 (has links)
No description available.
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Site Selection for Air Pollution Monitoring in the Vicinity of Point SourcesBrown, John C. 01 January 1978 (has links) (PDF)
Ever since air pollution became a national concern in the 1950's, more and more emphasis has been placed on collection of representative air samples for many purposed, to include (1) evaluation of the degree to which national ambient air quality standards are being met and (2) to monitor maximum emission levels from point sources. Until recently efforts were directed toward qualitative methods of siting monitors for representative sampling. Since the dispersion of effluents is most complex, the quality of the data collected on the basis of judgment and, more or less, incremental siting about the source, has become suspect. Furthermore, with the increasing demands for monitoring due to international growth in network monitoring systems, amendments to the Clean Air Act and the legislation on the Prevention of Significant Deteoriation of Air Quality, it is not cost-effective to encircle point sources with large numbers of equally spaced monitors. This paper discussed the history of air pollution concerns that have resulted in the need for monitoring; the development of siting techniques through largely qualitative measures; and finally, summarizes three quantitative methodologies for monitoring point sources. Emphasis is placed on the methodology developed by Noll, et al., (1977), based on the author's belief that this methodology represents the state of the art.
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