Garry oak (Quercus garryana Douglas ex. Hook) is a white oak (Quercus sect. Quercus) with a geographic range extending from southwestern BC to south-central California. It is the only native white oak in BC and Washington, and is the northernmost species of the California Floristic Province-Pacific Northwest white oak clade. I used molecular methods to address the following questions: 1) What are the patterns of genetic variation within Garry oak? 2) How do these patterns vary geographically, and how did the spatial distribution of the gene lineages come to occupy its current geographical range? 3) Does Garry oak show evidence of genetic interaction with other white oak species in western North America? 4) Is there morphological or genetic evidence to support the three described varieties of Garry oak? I obtained samples of Garry oak from 117 localities over its geographic range, as well as samples of two other California white oaks (Q. lobata and Q. douglasii) and a Rocky Mountain species (Q. gambelii). Analyses of DNA sequence data from four plastid DNA regions revealed 24 distinct molecular variants (haplotypes) in Garry oak. These show a strong south-to-north decrease in genetic diversity, consistent with post-glacial northward expansion. Haplotypes present in the northern part of the range provide evidence of two separate northward migrations, only one of which reached the northern range limit of Garry oak in BC. I found that Garry oak shared plastid DNA haplotypes with two other white oak species, indicating that it hybridizes with other oaks in the southern part of its range. The nuclear ribosomal ITS phylogeny showed poor resolution, but both cpDNA and nrDNA may indicate that Q. garryana is more closely related to the white oaks of central North America than was previously thought. My findings also suggest that the three currently recognized varieties of Garry oak (var. garryana, breweri and semota) are not well differentiated genetically, but show morphological variation at the regional level. This study shows the phylogeographic patterns within Q. garryana. In addition, it contributes to conservation efforts in Garry oak ecosystems by indicating regions of high genetic diversity in Garry oak, including genetically unique populations that may be especially worthy of preservation. / Graduate

Graduation date: 2006 / Habitat loss causes a reduction in available resources for wildlife, alters the configuration of remaining habitat, and may isolate wildlife populations. White-breasted nuthatches (Sitta carolinensis) are experiencing long-term population declines in the Willamette Valley of Oregon, where they are historically associated with oak woodlands. As secondary cavity-nesters, white-breasted nuthatches may be limited by the availability of existing cavities for nesting and roosting. Oak vegetation in the Willamette Valley has changed since European-American settlement times from vast areas of open oak savanna to isolated closed-canopy stands separated by agricultural fields. We examined nuthatch density, nest cavity selection, and nest success in relation to oak woodland structure and landscape context. We conducted point transect surveys in 3 strata: woodland interiors, large woodland edges, and small woodlands. We located and monitored nuthatch nests and sampled vegetation at nest locations and matching random locations around each nest. Woodland structure and edge density were measured at a 178-m radius (home range) scale, and landscape context was measured using vegetation cover within a 1-km radius around point transects and nests. We used program DISTANCE to fit detection functions and calculate nuthatch densities. We used conditional logistic regression to compare nest locations with random locations, and analyzed nest success with Mayfield logistic regression. White-breasted nuthatch density was significantly higher in small woodlands than in edges of large woodlands, which had higher nuthatch density than woodland interiors. Density of nuthatches increased with a combination of oak cover within a 1-km radius of the point, edge density within a 178-m radius, and number of oak trees >50 cm diameter at breast height (dbh) within a 100-m radius. Nest cavities were situated in oak trees containing more cavities than random oak trees that had cavities, and oak trees used as nest trees had a larger dbh than oak trees within random plots. Local woodland structure at nest locations was characterized by larger trees, measured by greater mean dbh, canopy cover, and basal area of oaks than random locations within the home range. Nest success in natural cavities was 71% and was not predicted by attributes of nest cavities, nest trees, local woodland structure at nests, woodland structure at the home range scale, or landscape context. These results suggest that the most suitable habitat for white-breasted nuthatches in the Willamette Valley includes oak woodlands in close proximity to one another with a high proportion of edge and mature oak trees. Managers should preserve trees containing cavities and large oak trees whenever possible. Thinning of small oaks and removal of conifers in oak woodlands to create more open, savanna-like conditions may also promote the development of larger oaks with more spreading branches, providing more opportunities for cavities to form and more foraging surface area for nuthatches.