Rhodococcus rhodochrous is an aerobic, non- pathogenic ,gram-positive bacterium that is often used in industries as a biocatalyst.R. rhodochrous DAP 96253 is capable of exhibiting contact-independent inhibition of selected fungal pathogens.The use of R. rhodochrous as a potential biocontrol agent against plant and animal fungi was examined.The fungi tested were Botrytis cinerea,Pseudogymnoascus destructans,Aspergillus flavus, Fusarium oxysporum’Cigar Tip’ , Rhizopus stolonifer’D1’ ,and other species isolated from berries.Each species was studied to establish the effect of dose (g/cells) and time of exposure to R. rhodochrous.Antifungal inhibition tests were done with the use of dosing,agar diffusion, frozen fermentation paste and exposed slides.Inhibition was observed with B.cinerea,P.destructans,A.flavus and D1,and reduced sporulation was observed with Cigar Tip. The results varied amongst the type of tests used on each target species.