Thesis (Ph. D.)--University of Washington, 1983. / Vita. Bibliography: leaves [201]-228.

Thesis (Ph. D.)--Oregon State University, 2001. / Typescript (photocopy). Includes bibliographical references. Also available on the World Wide Web.

Thesis (Ph. D.)--Washington State University, December 2009. / Title from PDF title page (viewed on Dec. 9, 2009). "College of Veterinary Medicine." Includes bibliographical references.

Some of the most devastating plant and animal pathogens belong to the oomycetes. Saprolegnia parasitica, a major pathogen of freshwater fish and the causal agent of Saprolegniosis, causes large economic losses in aquaculture due to the death of freshwater fish which contract Saprolegniosis. Losses due to Saprolegniosis are also incurred by natural fish populations. Saprolegniosis was previously controlled through the use of a biocide called malachite green, until the dye was found to have potentially carcinogenic effects. Following this discovery, the use of malachite green on fish destined to enter the food chain was banned worldwide and subsequently there has been an increase in the occurrence of Saprolegniosis. This work investigates various aspects of S. parasitica with the view to inform the development of new control strategies. Three cDNA libraries, including two interactive libraries, were analysed and bioinformatics provided valuable insight into the genes expressed not only by S. parasitica but also by the host. The annotation and expression profiles of several of these genes are described in this work and the potential of S. parasitica genes as vaccine targets is discussed. One protein, identified as a vaccine candidate, is annotated, cloned and overexpressed in order to evaluate its efficacy as a vaccine. It has been shown previously that the plant pathogenic oomycete Phytophthora infestans is able to utilise a protein localisation motif, the RxLR, in order to translocate effector proteins into the host. The question as to whether S. parasitica possesses a similar translocation system is addressed in this work. Localisation studies on an RxLR protein of S. parasitica origin are described and the microscopic interaction between S. parasitica and its host are described and attempts to optimise production of interaction structures are discussed.

No description available.

This paper is an investigation into the way that paranoia is represented in Thomas Pynchon's novel Gravity's Rainbow. Using various definitions of paranoia which are given in the text itself, I outline how each definition is demonstrated, both in narrative events and in the structural principles of the text. As well, I show how each definition may lend a different perspective on the reading process itself, thus implicating the Pynchon's reader in the paranoid dynamic which Gravity's Rainbow depicts. In effect, I attempt to return the pluralism to Pynchon's definition of paranoia.

No description available.

No description available.

This study of Gravity's Rainbow replies to critics who have misread Thomas Pynchon's novel, and defines the novel's focal concept. In Gravity's Rainbow, style, structure, and concept are integrated in the author’s design. The fulfillment of this design results in a comprehensive representation of the apocalyptic temper of western civilization in the modern period—the consciousness that has caused the armageddon of World War II and the threat of nuclear war. A meaningful term for this apocalypse is “parousia”, an end to history taking the form of a general surrender to deathliness. A beneficial way of defining “parousia” is through a certain ideological social stratification. Characters in the novel can be categorized as the Elect, the Preterite, or the Redeemer. Ethical struggles between these classes result in an on-going historical process toward an apocalypse.This paper organizes Pynchon’s apocalyptic concept into a centrifugal axis where meaning is organically interrelated and then spirals outward toward varied novelistic developments which offer other perspectives on the same basic concepts. The thesis explicates approximately thirty episodes which substantiate the “parousia” concept as it appears in the author’s style, structure, and thematic ideology.

A comprehensive survey of representative rainbow trout (Oncorhynchus mykiss) hatcheries revealed that the Gram negative, yellow-pigmented, filamentous bacterium Cytophaga psychrophila was implicated in a single disease in the U.K. and other European states. The involvement of C. psychrophila as the aetiological agent of the syndrome was substantiated by the fulfilment of Koch's postulates. Infectivity studies with isolates of C. psychrophila, carried out under natural and laboratory conditions successfully reproduced clinical signs and gross pathological changes analogous to those observed during field outbreaks of the condition. Histopathological examination of artificially and naturally infected fish tissues demonstrated several features that were considered to be pathognomonic for RTFS. Preliminary electron microscopic studies described the ultra-structure of C. psychrophila and partially elucidated the cellular response to the pathogen. Environmental sampling across selected hatchery sites demonstrated that members of the family Cytophagaceae formed a substantial element of the bacterial flora from natural waters, although recovery of C. psychrophila was restricted to areas where the substantial mortalities in fry attributable to RTFS had occurred. C. psychrophila was isolated from the sexual fluids of broodstock and additionally the bacterium was demonstrated associated with surfaces of eyed ova following various disinfection regimes. The minimum inhibitory concentrations of a range of antimicrobial agents both existing in, and novel to, aquaculture were examined, revealing compounds which would potentially mitigate losses attributable to RTFS during field outbreaks. The emergence of bacterial resistance to chemotherapeutants was discussed. The minimum inhibitory and bactericidal concentrations and required exposure times to a number of disinfecting agents were demonstrated. The efficacy of these agents as disinfectants of egg surfaces and equipment associated with fish production was assessed. The potential of a number of serodiagnostic techniques were evaluated as a means of rapid detection of C. psychrophila in diseased fish.