The strategy required for the development of the immunochemical reagents needed for a non-competitive assay for the detection of haptens was investigated. For this work, these reagents were termed "anti-complex" antibodies. To preserve the integrity of such an assay, these "anti-complex" antibodies should not react with either the free anti-hapten antibody or the free hapten and they should not compete with the hapten for binding to the anti-hapten antibody. Anti-hapten hybridomas were developed using standard hybridoma technology. The development of anti-complex hybridomas was investigated using standard hybridoma technology and the novel approach of intrasplenic immunization. Both immunization protocols addressed the three questions concerning the strategy for developing such hybridomas, namely the type of immunization (snygeneic or allogeneic), the physico-chemical nature of the hapten in the complex (free or conjugated) and the physico-chemical nature of the anti-hapten antibody in the complex (intact or fragmented). (Abstract shortened with permission of author.)

published_or_final_version / Chemistry / Master / Master of Philosophy

No description available.

by Lawrence Tin-chi Law. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1993. / Includes bibliographical references (leaves 96). / acknowledgements --- p.iii / ABSTRACT --- p.iv / CONTENTS --- p.v / ABBREVIATION --- p.vii / Chapter CHAPTER I --- METALLATION OF 8-METHYLQUINOLINE / Chapter 1.1 --- INTRODUCTION --- p.1 / Chapter 1.1.1 --- A Brief Review of Metal-Alkyl Chemistry --- p.1 / Chapter 1.1.2 --- General Considerations --- p.2 / Chapter 1.1.3 --- 8-Methylquinoline as Ligand Precursor --- p.8 / Chapter 1.1.4 --- Metallations by Organolithium Compounds --- p.9 / Chapter 1.1.5 --- Other Methods for Metallations --- p.16 / Chapter 1.1.6 --- Aim of the Present Work --- p.21 / Chapter 1.2 --- RESULTS AND DISCUSSION --- p.24 / Chapter 1.2.1 --- Reactions of 8-Methylquinoline with Organolithium Reagents --- p.26 / Chapter 1.2.2 --- Synthesis of Grignard Reagent --- p.35 / Chapter 1.2.3 --- Attempted Metal-Halogen Exchange Reaction at Low Temperature --- p.39 / Chapter 1.2.4 --- Metallation of 8-methylquinoline by Lithium Diisopropylamide --- p.40 / Chapter 1.3 --- EXPERIMENTAL FOR CHAPTER I --- p.43 / Chapter 1.4 --- REFERENCES FOR CHAPTER I --- p.53 / Chapter CHAPTER II --- SYNTHESIS AND CHARACTERISATION AND STRUCTURE OF SOME MAIN GROUP 14 ALKYLS / Chapter 2.1 --- INTRODUCTION --- p.58 / Chapter 2.1.1 --- General Aspects of Group 14 Organometallic Compounds --- p.58 / Chapter 2.1.2 --- Group 14 Organometallic Confounds --- p.59 / Chapter 2.1.3 --- Subvalent Group 14 Metal Alkyls --- p.63 / Chapter 2.2 --- RESULTS AND DISCUSSION --- p.67 / Chapter 2.2.1 --- Synthesis of Five Co-ordinated Tin (IV) Compound --- p.67 / Chapter 2.2.2 --- Molecular Structure of [Sn{8-(CHSiMe3)C9H6N}Ph2Cl] --- p.70 / Chapter 2.2.3 --- Synthesis of Group 14 Subvalent Metal Complexes --- p.74 / Chapter 2.3 --- EXPERIMENTAL FOR CHAPTER II --- p.76 / Chapter 2.4 --- REFERENCES FOR CHAPTER II --- p.79 / Chapter CHAPTER III --- SYNTHESIS AND CHARACTERISATION OF SOME GROUP 12 (ZINC AND CADMIUM) METAL DIALKYLS / Chapter 3.1 --- INTRODUCTION --- p.81 / Chapter 3.1.1 --- A General Aspect of Group 12 Organometallics --- p.81 / Chapter 3.2 --- RESULTS AND DISCUSSION --- p.88 / Chapter 3.2.1 --- Synthesis of Group 12 Organometallic Confounds --- p.88 / Chapter 3.2.2 --- Molecular Structure of [Cd{8-(CHSiMe3)C9H6N}(tmeda)Cl] --- p.91 / Chapter 3.3 --- EXPERIMENTAL FOR CHAPTER III --- p.93 / Chapter 3.4 --- REFERENCES FOR CHAPTER III / APPENDIX I / Chapter 1. --- GENERAL PROCEDURES --- p.97 / Chapter 2. --- PHYSICAL AND ANALYTICAL MEASUREMENTS --- p.100 / APPENDIX II / LIST OF SELECTED 1H NMR SPECTRA --- p.101

by Zhi-Jie Ni. / Thesis (Ph.D.)--Chinese University of Hong Kong, 1989. / Bibliography: leaves 143-153.

Thesis (Ph. D.)--University of California, San Diego and San Diego State University, 2002. / Vita. Includes bibliographical references (leaves 124-134).

published_or_final_version / Chemistry / Doctoral / Doctor of Philosophy

No description available.

No description available.

No description available.