1 |
H19 and miR-675 : a long noncoding RNA conceals a growth suppressing microRNAKeniry, Andrew James January 2012 (has links)
No description available.
|
2 |
Light activated RNA interferenceShah, Samit, Friedman, Simon H. January 2007 (has links)
Thesis (Ph. D.)--School of Pharmacy and Dept. of Chemistry. University of Missouri--Kansas City, 2007. / "A dissertation in pharmaceutical science and chemistry." Advisor: Simon H. Friedman. Typescript. Vita. Description based on contents viewed July 16, 2008; title from "catalog record" of the print edition. Includes bibliographical references (leaves 206-220). Online version of the print edition.
|
3 |
The transient receptor potential channel 1 (TRPC1) mediates calcium-regulated differentiation in oral gingival keratinocytes /Cai, Shiwei. January 2004 (has links)
Thesis (Ph. D.)--University of Washington, 2004. / Vita. Includes bibliographical references (leaves 113-124).
|
4 |
Inhibition of ErbB2 and Thymidylate Synthase by a Multi-Targeted Small-Interfering RNA in Human Breast Cancer Cell LinesHunter, Rebecca Stephanie 14 February 2008 (has links)
The therapeutic potential of a novel multi-targeted small-interfering RNA (siRNA) was investigated in human breast cancer cells. Previous studies had identified an siRNA that specifically and potently inhibited expression of thymidylate synthase (TS) by directly targeting human TS mRNA. TS is a folate-dependent enzyme that catalyzes the key reaction involved in synthesizing nucleotide precursors for DNA biosynthesis, and as such, it plays a critical role in maintaining cell growth. The goal of this thesis was to design and develop a novel siRNA molecule that targeted TS mRNA as well as a cellular mRNA that encodes a different cellular protein involved in cancer cell growth and proliferation, such as a member of the ErbB family. Gene sequence analysis was performed and identified an overlapping sequence between TS and ErbB2 mRNAs. An siRNA duplex was then designed to simultaneously target human TS and ErbB2 mRNA. Transfection of the multi-targeted siRNA (TS1M17) revealed that both ErbB2 and TS proteins were significantly suppressed in a time and dose-dependent manner in ErbB2-overexpressing human breast cancer SKBR3 cells. The corresponding mRNA levels, as determined by RT-PCR, were also decreased. Protein levels of other ErbB family members, including ErbB1 and ErbB3, remained unchanged with siRNA treatment. An ErbB2-specific siRNA (B2450) inhibited ErbB2, but had no effect on TS expression demonstrating the specificity of the multi-targeted siRNA against both TS and ErbB2. Mismatched (TS1-Mismatch) and control (GL2) siRNAs had no inhibitory effects on expression of the two target proteins. Suppression of activated ErbB2, as determined by expression of phosphorylated ErbB2 protein, was observed with transfection of TS1M17 siRNA. In addition, the expression of downstream signaling proteins, such as phosphorylated mitogen activated protein kinase (p-MAPK), p27Kip1, p21Cip1, cyclin D1, and survivin were significantly changed. In contrast, control siRNAs did not exert any inhibitory effects on downstream signaling. Taken together, these findings suggest that TS1M17 siRNA inhibits signaling of the ErbB2 pathway. The effect of TS1M17 siRNA on cytotoxicity was analyzed by WST-1 assay. Upon transfection into SKBR3 cells, the TS1M17 siRNA significantly suppressed cell proliferation with an IC50 value of 0.65 nM, which is 154-fold more potent than ErbB2- and TS-specific siRNAs. This study suggests that targeting expression of ErbB2 and TS, two key proteins involved in distinct and critical pathways for cancer growth and proliferation, with a single siRNA molecule may provide a novel approach for cancer chemotherapy.
|
5 |
Interference with HIV-1 primer selection by siRNA directed to the HIV-1 primer binding siteHan, Wenlong. January 2006 (has links) (PDF)
Thesis (Ph. D.)--University of Alabama at Birmingham, 2006. / Title from first page of PDF file (viewed Feb 15, 2008). Includes bibliographical references.
|
6 |
To develop a small interfering RNA (siRNA) design and information resource to facilitate manipulation of human cells.Shah, Jyoti Khetsi January 2006 (has links)
Thesis (M.S.) -- University of Texas Southwestern Medical Center at Dallas, 2006. / Vita. Bibliography: p. 98-102.
|
7 |
Nucleic acid based therapeutic approaches /Elmén, Joacim, January 2005 (has links)
Diss. (sammanfattning) Stockholm : Karol. inst., 2005. / Härtill 4 uppsatser.
|
8 |
Linear and branched chitosan oligomers as delivery systems for pDNA and siRNA in vitro and in vivo /Issa, Mohamed Mahmoud, January 2006 (has links)
Diss. (sammanfattning) Uppsala : Uppsala universitet, 2006. / Härtill 4 uppsatser.
|
9 |
High resolution genomic tools for the discovery of protein function in mammalian cells /Hodges, Emily Carol, January 2006 (has links)
Diss. (sammanfattning) Stockholm : Karol. inst., 2006. / Härtill 6 uppsatser.
|
10 |
The use of RNA technologies to decrease HIV-1 replication and investigate the role of RNA interference in the innate immune response to HIV-1 infectionSoye, Kaitlin Jane. January 1900 (has links)
Thesis (M.Sc.). / Written for the Dept. of Microbiology and Immunology. Title from title page of PDF (viewed 2008/05/29). Includes bibliographical references.
|
Page generated in 0.1178 seconds