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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

RNAlater som bevaringsmetod för muskelvävnad : En jämförande metodstudie om frystorkning, RNAlater och RNAlater-ICE som bevaring av skelettmuskulatur inför molekylärbiologiska analyser / RNAlater as a Method for Preservation of Muscle Tissue : A Comparative Method Study on Lyophilization, RNAlater and RNAlater ICE for Preservation of Human Skeletal Muscle Preceding Molecular Biological Analyzes

Engvall, Alice, Eriksson-Viklund, Tuva January 2022 (has links)
I denna metodstudie jämfördes Thermo Fishers bevarande lösningar RNAlater och RNAlater-ICE med frystorkning som bevaring av skelettmuskulatur från människa. Studien gjordes med syftet att avgöra om RNAlater och/eller RNAlater-ICE kan ersätta frystorkning, i hopp om att underlätta dissekering av muskelvävnad. De molekylärbiologiska analyser som utfördes var proteinbestämning; Western Blot inriktad på proteinerna mTor, S6, S6K1, eEF2, myosin typ II och aktin; samt mätning av glykogen och citratsyntasaktivitet. Därtill påbörjades även en aminosyraanalys. Studien utfördes hos William Apró på Åstrandlaboratoriet på Gymnastik- och idrottshögskolan i Stockholm. Resultaten visade att både RNAlater och RNAlater-ICE är olämpliga att använda i studier som innefattar samtliga av de genomförda analyserna. Detta då analysresultaten från de alternativa bevaringsmetoderna och de från frystorkningen inte var likvärdiga. Därmed drogs slutsatsen att varken RNAlater eller RNAlater-ICE kan ersätta frystorkning som bevaringsmetod i Aprós vidare studier. / In this method study Thermo Fisher’s preserving solutions RNAlater and RNAlater-ICE were compared to lyophilization for preservation of human skeletal muscle. The study was conducted with the aim of determining whether RNAlater and / or RNAlater-ICE can replace lyophilization, in the hope of facilitating dissection of muscle tissue. The molecular biological analyzes performed were protein assay; Western Blot focused on the proteins mTor, S6, S6K1, eEF2, myosin type II and actin; alongside of measurments on glycogen and citrate synthase activity. In addition, an amino acid analysis was initiated. The study was executed with William Apró at the Åstrand Laboratory at The Swedish School of Sport and Health Science in Stockholm. The results showed that both RNAlater and RNAlater-ICE are unsuitable for use in studies that include all of the analyzes performed. This is because the analysis results from the alternative preservation methods and those from lyophilization were not equivalent. Thus, it was concluded that neither RNAlater nor RNAlater-ICE can replace lyophilization as a preservation method in Apró’s further studies.

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