Previous studies on wood instability have identified some wood basic properties which influence the timber distortion and shape changes while the wood is losing or gaining moisture. These properties include wood anisotropic shrinkage, equilibrium moisture content (EMC) in both desorption and adsorption, fibre saturation point (FSP) and water diffusion coefficient. This study investigated the stability related wood properties and their variations within the stems of the New Zealand grown radiata pine trees. The basic density, EMC and shrinkage for the earlywood and the latewood were also experimentally determined. In taking into account these properties and their variations, an analytical model was developed to simulate the wood distortion. The equilibrium moisture content was determined at 30°C and humidity range from 27% to 90%. It was found that the EMC difference between the earlywood and the latewood was negligible. The experimental results also show that the EMC decreases from pith towards the bark over the disc cross section and the trend is most obvious at high humidities (70-90%). Along the tree height, the EMC decreases from ground towards the top of the stem. The tangential shrinkage results were highly variable but, in general, the latewood has higher values than the earlywood. The opposite trend was found for the longitudinal shrinkage, higher values for the earlywood than the latewood. In the tree height direction, the tangential shrinkage was found to decrease with the tree height. This follows the same trend as the microfibril angle which in general also decreases with the tree height. The fibre saturation point (FSP) was determined from the tangential shrinkage values at different equilibrium moisture contents. These were taken as the intersection point of the tangential shrinkage line with the moisture content axis. The experimental results have revealed a trend that the FSP decreases from the pith towards the bark and also decreases with the tree height. Similar behaviour was found for the tangential and longitudinal swelling during the adsorption. The moisture transport within radiata pine was investigated based on the diffusion theory under transient and isothermal condition of 30°C. The moisture dependant diffusion coefficient was derived from the experimental data using the inverse analysis method. The surface emission coefficient was determined by taking into account the variable surface moisture content, wood density and the ambient conditions. In the experiments, 138 samples in total (46 samples for each XIV of the longitudinal, tangential and radial directions) were used and their diffusion coefficients were determined for both the adsorption and the desorption with moisture content ranging from 6% to 22%. The derived diffusion coefficients are comparable with the reported data in the literature. Further investigation of the wood type influence on the diffusion coefficient revealed that compression wood has the lowest diffusion coefficients in the three directions (longitudinal, tangential and radial) both in the desorption and in the adsorption. In addition, the moisture content has the least influence on the diffusion coefficient in the compression wood. Investigation on the effects of the wood density has shown that in general, the diffusion coefficient decreases with increasing wood density although the correlations are only significant for the longitudinal direction in desorption. An analytical model for the simulation of the wood twist was developed which is based on the geometry and geometrical changes of the grains aligning non parallel to the pith. A board consists of numerous such grains which change the length and shape with moisture content change. The model predictions confirm that the grain angle, the tangential and longitudinal shrinkage and the conical angle all affect the development of the board twist. However, the discrepancies between the model prediction and the experimental data are significant, partly due to the experimental uncertainties and partly due to the model errors. In this study, sound velocity in the longitudinal direction was measured for the test samples used in the shrinkage experiments. The results revealed that the correlations between the sound velocity and the volumetric shrinkage at oven dry are significant. Based on this finding, the relationship between the tangential shrinkage and the radial shrinkage, the tangential shrinkage can be predicted from the sound velocity measurements. As the tangential shrinkage has significant influence on the wood distortion, this method can be used to segregate logs which may be prone to wood distortion.

Interactions between plant roots and soil microorganisms in the rhizosphere are critical for plant growth. However, understanding of precisely how root exudates influence the diversity and activity of rhizosphere microorganisms is limited. The main objective of this study was to investigate the effect of radiata pine (Pinus radiata) root exudates on rhizosphere soil microbial communities, with an emphasis on the role of low molecular weight organic anions. The study involved the development and validation of new methods for investigating rhizosphere processes in a purpose-built facility. This included development of an in situ sampling technique using an anion exchange membrane strip to collect a range of organic anions exuded from radiata pine roots grown in large-scale rhizotrons. These included tartarate, quinate, formate, malate, malonate, shikimate, lactate, acetate, maleate, citrate, succinate and fumarate. Soil microbial activity and diversity were determined using dehydrogenase activity and denaturing gradient gel electrophoresis. Links between organic anions in root exudates and rhizosphere soil microbial community structures were investigated by comparing wild type and genetically modified radiata pine trees which were grown in rhizotrons for 10 months. As expected, there was considerable temporal and spatial variability in the amounts and composition of organic anions collected, and there were no consistent or significant differences determined between the two tree lines. Significant differences in rhizosphere microbial communities were detected between wild type and genetically modified pine trees; however, they were inconsistent throughout the experiment. The shifts in microbial communities could have been related to changes in exudate production and composition. Based on results from the main rhizotron experiment, a microcosm study was carried out to investigate the influence of selected pine root exudate sugars (glucose, sucrose and fructose) and organic anions (quinate, lactate and maleate) on soil microbial activity and diversity. Soil microbial activity increased up to 3-fold in all of the sugar and organic anion treatments compared to the control, except for a mixture of sugars and maleate where it decreased. The corresponding impacts on soil microbial diversity were assessed using denaturing gradient gel electrophoresis and 16S rRNA phylochips. Addition of the exudate compounds had a dramatic impact on the composition and diversity of the soil microbial community. A large number of bacterial taxa (88 to 1043) responded positively to the presence of exudate compounds, although some taxa (12 to 24) responded negatively. Organic anions had a greater impact on microbial communities than sugars, which indicated that they may have important roles in rhizosphere ecology of radiata pine. In addition, a diverse range of potentially beneficial bacterial taxa were detected in soil amended with organic anions, indicating specific regulation of rhizosphere microbial communities by root exudates. This project highlighted the considerable challenges and difficulties involved in detailed investigation of in situ rhizosphere processes. Nonetheless, the findings of this study represent a significant contribution to advancing understanding of relationships between root exudates and soil microbial diversity, which will be further enhanced by refinement and application of the specific methodologies and techniques developed.