Call number: LD2668 .T4 1979 M34 / Master of Science

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The purpose of this study is to determine the optimum conditions for obtaining a leukocyte button which most effectively will be subsequently labelled with 111In oxine. As in all radiopharmaceuticals, the highest radiopharmaceutical purity, or the fraction of total radioactivity in the desired radiopharmaceutical form (111In oxine leukocyte), the better the product. Many 111In ocine labelled leukocytes are contaminated by labelled platelets, red cells, and proteins, resulting in a “dirty” product. But with careful leukocyte culturing, sedimentation, centrifugation, and labelling, as demonstrated by this study, a highly desirable, pure radiopharmaceutical can be made. In an attempt to further purify the leukocyte button beyond centrifugation, hypotonic red cell lysing and its effect on leukocyte viability will be studied. The optimum incubation time will be determined by examining the leukocyte and red cell elution profiles at different incubation times. And, 0.9% saline washes of plasma and proteins from the leukocytes will be varied by both volume and number to determine if extra washes will optimize the labelling efficiency.

A thesis submitted to the faculty of Engineering, University of the Witwatersrand, Johannesburg , in fulfillment of the requirements for the degree of Doctor of Philosophy. Johannesburg, November 1994 / Organ function is often characterised using imaging techniques. In particular a tracer is often used which does not react with tissue, is low in concentration, follows body fluid flows and is distinguishable from the observed system and thus measurable. These requirements ensure linear characteristics of the tracer. In this thesis, these linear characteristics are used to develop a generalised mathematical theory to determine organ function from imaged tracer experiments. The theory is based on anatomical and physiological information for single and multiple input organs. [Abbreviated Abstract. Open document to view full version] / MT2018