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INVESTIGATING THE POTENTIAL APPLICATIONS OF A RAMAN TWEEZER SYSTEMWray, John 30 April 2013 (has links)
This thesis describes the construction of an Optical Tweezer apparatus to be used in conjunction with a confocal Raman spectrometer. The tweezer utilizes an infrared (λ=1064 nm) laser directed into an inverted microscope with NA=1.4 oil immersion 100x objective lens that strongly focuses the laser light into a sample to function as a single-beam gradient force trap. The long term goal of this research program is to develop a single molecule Raman tweezers apparatus that allows one to control the position of a Raman nanoplasmonic amplifier. This thesis describes the construction of the Raman tweezer apparatus along with several Raman spectra obtained from optically trapped samples of polystyrene fluorescent orange, amine-modified latex beads. In addition, I explored the Raman spectra of bulk cytochrome c mixed with or injected onto Ag aggregates for SERs enhancement.
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