Ganzzellsensoren sind potentielle Kandidaten für den Einsatz in der Umwelttechnik zur Detektion von Schwermetallen, organischen Lösungsmitteln oder Xenobiotika. Ebenso können mit ihrer Hilfe andauernde Prozesse, wie z.B. in der Biogasentwicklung, überwacht werden. Etablierte, auf Genexpression basierende Ausleseverfahren besitzen unterschiedliche Nachteile, die den Einsatz bisher weitestgehend auf das Labor beschränken. In der vorliegenden Arbeit wurden dazu drei alternative Auslesemöglichkeiten evaluiert, die durch kostengünstige Messverfahren und einem einfachen experimentellen Aufbau realisiert werden können. Die gezielte Morphologieänderung von Sensorhefen, die analytinduzierte Aggregation von Zellen und die analytabhängige Anlagerung von Goldnanopartikeln an Sensorhefen. Während sich die zwei erstgenannten Verfahren derzeit noch in Entwicklungszustand befinden, konnte durch die Anlagerung von Goldnanopartikeln an Sensorhefen der Wirkstoff Diclofenac erfolgreich detektiert werden. Die dazu notwendige Inkubationsdauer der Sensorhefen mit Diclofenac als auch das Detektionslimit wurde im Vergleich zu veröffentlichten Daten um je eine Größenordnung verringert.

<p>The compressive sensing (CS) paradigm provides an efficient image acquisition technique through simultaneous sensing and compression. Since the imaging philosophy in CS imagers is different from conventional imaging systems, new physical structures are required to design cameras suitable for CS imaging.</p> <p>While this work is focused on the hardware implementation of CS encoding for CMOS sensors, the image reconstruction problem of CS is also studied. The energy compaction properties of the image in different domains are exploited to modify conventional reconstruction problems. Experimental results show that the modified methods outperform the 1-norm and TV (total variation) reconstruction algorithms by up to 2.5dB in PSNR.</p> <p>Also, we have designed, fabricated and measured the performance of two real-time and area-efficient implementations of the CS encoding for CMOS imagers. In the first implementation, the idea of active pixel sensor (APS) with an integrator and in-pixel current switches are used to develop a compact, current-mode implementation of CS encoding in analog domain. In another implementation, the conventional three-transistor APS structure and switched capacitor (SC) circuits are exploited to develop the analog, voltage-mode implementation of the CS encoding. With the analog and block-based implementation, the sensing and encoding are performed in the same time interval, thus making a real-time encoding process. The proposed structures are designed and fabricated in 130nm technology. The experimental results confirm the scalability, the functionality of the block read-out, and the validity of the design in making monotonic and appropriate CS measurements.</p> <p>This work also discusses the CS-CMOS sensors for high frame rate CS video coding. The method of multiple-camera with coded exposure video coding is discussed and a new pixel and array structure for hardware implementation of the method is presented.</p> / Doctor of Philosophy (PhD)

This dissertation deals with the issue of reading skills of pre-school aged children, their abilities to understand the text being read out to them and answer the questions related to the text meaningfully, but also to ask individual questions. It addresses a possible usage of some methods of critical thinking for this age group in the field of nursery schools. The dissertation is based on the fact that the role of the pre-school education is irreplaceable in this respect. The theoretical part concentrates on the problems related to answering the questions and asking them, on the development of cognitive processes and speech, growth of self- confidence and self-assurance as an important precondition for a pre-school child's expressing and the creation of a positive pedagogical communication in the class. It offers opportunities to use some methods of the programme called " By Reading and Writing to Critical Thinking " (RWCT) at nursery schools. The practical part of this dissertation introduces a programme based on working with the texts of different genres intended for the pre-school age. In individual lessons it is verified whether the chosen methods from the RWCT programme are suitable and if the questions asked, and the request for asking them, correspond to the abilities of pre-school aged...

La matière sombre compte pour 85% de la matière composant l’univers et nous ne savons toujours pas ce qu’elle est. Depuis plusieurs années, l’expérience Picasso, maintenant devenue l’expérience PICO, tente d’élucider ce mystère. Les fréons de la famille des CXFY sont utilisés comme cibles de choix dans les détecteurs à liquides surchauffés de l’expérience PICO. Situés à SNOLab, en Ontario, ces détecteurs font parties des plus performant de la recherche de la matière sombre. Lors d’interactions de particules avec le liquide en surchauffe, un changement de phase est induit par le dépôt d’énergie engendré par l’interaction. Les bulles créées par l’évènement sont alors détectées par différents capteurs afin de déterminer le type d’interaction qui a eu lieu. Dans ce travail seront présentés les détecteurs à liquides surchauffés dans le cadre de la recherche de la matière sombre. Principalement, nous y verrons trois types de détecteurs utilisés par les expériences PICO et Picasso. Le principe de fonctionnement de chacun des détecteurs sera exposé en premier lieu ainsi que leur fabrication, puis leur mode d’opération et l’analyse des données. Les méthodes de calibration seront par la suite expliquées pour terminer avec une description des résultats obtenus démontrant la performance de ce type de détection. / Dark matter makes up 85% of the matter content of the universe and we still don’t know what it is made of. The Picasso experiment, now named PICO, has been searching for it for several years with the use of superheated liquid detectors. Following the interaction of a particle with a superheated liquid freon of the CXFY family, a bubble is formed through a phase change and is detected with several types of sensors, telling us about the nature of the event. Located at SNOLab, in Ontario, these detectors produce some of the best results in the field. The present work will go through three types of superheated liquid detectors. A full description of the working principles will be presented for each of them. In addition, the fabrication, the operation mode and the data analysis will be shown. Detector calibration techniques will then be presented with different particle sources. Finally, the most recent results will be discussed, demonstrating the performance of the superheated liquid detector technique.