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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

In vitro studies on the mechanism of homologous DNA recombination promoted by Escherichia coli RecA protein

黃楚華, Wong, Choi-wah, Brian. January 1993 (has links)
published_or_final_version / Biochemistry / Doctoral / Doctor of Philosophy
2

In vitro studies on the mechanism of homologous DNA recombination promoted by Escherichia coli RecA protein /

Wong, Choi-wah, Brian. January 1993 (has links)
Thesis (Ph. D.)--University of Hong Kong, 1993. / Photocopy from microfilm of typescript.
3

Some physiochemical characteristics of selected bovine caseins and cloning of DNA coding for bovine [beta]-casein

Zoerb, Hans Frederic. January 1983 (has links)
Thesis (Ph. D.)--University of Wisconsin--Madison, 1983. / Typescript. Vita. Includes bibliographical references.
4

Flp recombinase : structural and functional studies /

Chen, Yu. January 2002 (has links)
Thesis (Ph. D.)--University of Chicago, Dept. of Biochemistry and Molecular Biology, December 2002. / Includes bibliographical references. Also available on the Internet.
5

Studies on the 5' non-coding region of the genome of poliovirus

Sullivan, Michael A. January 1992 (has links)
The last decade has seen widespread application of recombinant DNA technology to the study of picornaviruses. Comparative sequence analysis has revealed that the most highly conserved region amongst many members of this family of viruses is the 5' non-coding region. Using recombinant type 3 polioviruses it has been shown that a single point mutation located in this region dramatically reduces neurovirulence and inhibits the intracellular life-cycle of the virus. Mutation at this nucleotide contributes to the observed reversion to neurovirulence of the Sabin attenuated poliovirus type 3 vaccine strain currently used in vaccination programmes throughout the world. Knowledge concerning the function of the 5' non-coding region remains scant, and as a result, the mechanism whereby a single point mutation within this region results in alteration of the expressed phenotype of the virus remains unknown. Clearly, an understanding of the molecular mechanism(s) involved requires greater knowledge of the function of the 5' non-coding region. This thesis describes the design and construction of vectors that allow analysis of the role of the 5' non-coding region in the control of viral translation, replication, and encapsidation of viral RNA. In the plasmid pRSV-5'polio-CATm2 (N+), the 5' non-coding region of poliovirus was fused to the coding region of the bacterial chloramphenicol acetyltransferase reporter gene. The presence of the 5' non-coding region resulted in the inhibition of CAT expression when this plasmid was introduced into eukaryotic cells in culture. Deletion analysis of the 5' non-coding region in this vector identified two regions that were responsible for the marked inhibition of expression of the reporter gene. It would appear from the results of these experiments that the poliovirus/CAT chimaeric message is translatsed as a normal eukaryotic mRNA and is subject to the rules of the "scanning model". This observation suggests that the 5' non-coding region of poliovirus on its own does not possess features which enable a message containing it to be translated efficiently. It is concluded that a second factor, present in infected cells, is required for the efficient translation of poliovirus. A second plasmid was designed and constructed to investigate the role of the 5' non-coding region in replication and encapsidation of viral RNA. Preliminary data suggest that the product of this vector does undergo replication while its ability to be encapsidated has still to be tested.
6

The electrokaryotype and molecular characterization of Trypanosoma cervi isolates using recombinant DNA techniques

Bennett, J. Lindsley 01 January 1990 (has links)
The distribution of trypanosomes infecting wild ruminants of North America has only recently been investigated . Many isolates have been mensurally studied and were determined to be conspecific with Trypanosoma cervi. Widely divergent forms exist however, between host species and seasonally within a host . To determine the validity of all inclusions in the taxon and the extent of intraspecific variability , trypanosome isolates of moose, reindeer , antelope, muledeer, Roosevelt Elk and two discrete transplant populations of Rocky Mountain elk were characterized and differentiated using recombinant DNA techniques.
7

Phylogenetic utility of ribosomal and protein-coding genes in Sordariomycetes systematics and evolutionary relationships within the Xylariaceae

Tang, Ming-chak. January 2007 (has links)
Thesis (Ph. D.)--University of Hong Kong, 2007. / Title proper from title frame. Also available in printed format.
8

The molecular cloning of bacteriophage T4: the identification and expression of a clone coding for bacteriophage thymidylate synthetase

DiRenzo, Anthony Benjamin January 1979 (has links)
No description available.
9

Left-handed Z-DNA in DNA restriction fragments and recombinant plasmids

Stirdivant, Steven Milton. January 1982 (has links)
Thesis (Ph. D.)--University of Wisconsin--Madison, 1982. / Typescript. Vita. Description based on print version record. Includes bibliographical references.
10

Construction of a recombinant cDNA library and isolation of a plasmid containing thymidylate synthetase cDNA sequences /

Geyer, Pamela Kent January 1983 (has links)
No description available.

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