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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.

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Spelling suggestions: "subject:"recombinationdeficient mutants"" "subject:"recombinationcoefficients mutants""

1

Genetic characterisation of Escherichia coli RecN protein as a member of SMC family of proteins

Youssef, M.M., Al-Omair, M.A., Picksley, Stephen M. 06 February 2011 (has links)
Yes / The proteins of SMC family are characterised by having Walker A and B sites. The Escherichia coli RecN protein is a prokaryotic member of SMC family that involved in the induced excision of Tn10 and the repair of the DNA double strand breaks. In this work, the Walker A nucleotide binding site of the E. coli RecN protein was mutated by changing the highly conserved lysine residue 35 to the aspartic acid (D), designated as recN(K35D). Reverse genetics was utilized to delete the entire recN gene (Delta recN108) or introduce the recN(K35D) gene into the E. coli chromosomal DNA. The recN(K35D) cells showed decreasing in the frequency of excision of Tn10 from gal76
RECN
SMC
tn10 excision
Recombination
Recombination-deficient mutants
Double-strand breaks
DNA-repair
Chromosome condensation
Exonuclease-i
k-12
Identification
Mutations
Strains
Product

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