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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

OcorrÃncia de bactÃrias diazotrÃficas em cactÃceas nativas do semiÃrido e seus efeitos no crescimento de mudas de Mandacaru. / Occurrence of diazotrophic bacteria in cacti from semiarid and its effects on growth of Mandacaru seedlings

Josà VinÃcius Leite Lima 28 February 2013 (has links)
CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior / A presenÃa de bactÃrias fixadoras de nitrogÃnio atmosfÃrico tem sido evidenciada em diferentes espÃcies, incluindo leguminosas, gramÃneas, frutÃferas, entre outras plantas. Tem-se por hipÃtese que as cactÃceas estabelecem naturalmente associaÃÃo com as bactÃrias diazotrÃficas endofÃticas e podem se beneficiar da interaÃÃo planta-bactÃria na fase de crescimento inicial. Este estudo teve por objetivo isolar e caracterizar bactÃrias diazotrÃficas associadas Ãs cactÃceas do semiÃrido e avaliar sua contribuiÃÃo no crescimento de mudas de Mandacaru. A anÃlise da ocorrÃncia de bactÃrias diazotrÃficas foi realizada em dezembro de 2011, coletando-se amostras de raÃzes de partes aÃreas de Cereus jamacaru e Melocactus zehntneri, na EstaÃÃo EcolÃgica (ESEC) de Aiuaba, CearÃ, Brasil. A presenÃa das bactÃrias endofÃticas foi confirmada nas cactÃceas nativas e as estirpes bacterianas caracterizadas pela fisiologia de crescimento em meios artificiais, sob condiÃÃo de fixaÃÃo biolÃgica de nitrogÃnio. Representantes destes grupos bacterianos foram inoculados em mudas micropropagadas de Mandacaru. ApÃs seis meses de cultivo em casa de vegetaÃÃo, os Mandacarus apresentaram crescimento similar independente da inoculaÃÃo com endÃfitos. Todas as bactÃrias isoladas apresentaram habilidade em solubilizar fosfato inorgÃnico no meio Pikoskaya. As bactÃrias endofÃticas oriundas dos cactos nativos diferem na forma de crescimento sob condiÃÃo de fixaÃÃo biolÃgica de nitrogÃnio e pelo uso de fontes de carbono. As sete estirpes bacterianas testadas nÃo influenciaram o crescimento inicial de Mandacarus em substrato composto de casca de arroz parcialmente carbonizada, vermiculita e vermicomposto. / The presence of nitrogen-fixing bacteria has been observed in various plant species, including legumes, grasses, and fruit plants. It has been hypothesized that the cactus from semiarid region naturally establishes association with endophytic bacteria and take advantages from plant-bacteria interaction in the initial growth phase. This study aimed to isolate and characterize diazotrophic from cactus in semiarid and evaluate their contribution on the growth of Mandacaru seedlings. The survey of occurrence of diazotrophs was held in December 2011, collecting samples from roots and aerial parts of Mandacaru (Cereus jamacaru) and Coroa-de-frade (Melocactus zehntneri) at the Ecological Station of Aiuaba, state of CearÃ, Brazil. The presence of endophytic bacteria was confirmed in native cacti and bacterial strains characterized by the physiology of growth on artificial medium, on condition of biological nitrogen fixation. Representative strains from homogeneous bacterial groups were used to inoculate Mandacaru seedlings. After six months of growing in greenhouse, the Mandacaru seedlings showed similar growth independently of bacterial inoculation. All bacteria obtained cacti showed ability showed ability to solubilize inorganic phosphate in medium Pikoskaya. The endophytes obtained differ in growth condition under nitrogen fixation and by using different carbon sources. The seven bacterial strains tested did not affect significantly the initial growth of Mandacaru in substrates with rice hulls partially burned, vermiculite and humus.

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