Thesis (Ph. D.)--Oregon State University, 1973. / Typescript (photocopy). Includes bibliographical references. Also available on the World Wide Web.

by Hon-ming Lam. / Thesis (M.Ph.)--Chinese University of Hong Kong, 1987. / Bibliography: leaves 193-217.

Legislation to reduce open field burning in grass seed fields within the Willamette valley of western Oregon changed established production practices. In the creeping grasses such as Kentucky bluegrass (Poa pratensis L.) and creeping red fescue (Festuca rubra L.) non-thermal management resulted in reduced yield. Studies were conducted to examine the effects of three stubble height treatments in comparison to open field burning in creeping red fescue seed production. The effects of light quality on characteristics of plant development were investigated in field and controlled environments. Exogenous applications of plant growth regulators (PGR's) were made to elucidate the causes of low seed yields observed without burning. Field plots were prepared in fall of 1994, and 1995 in creeping red fescue commercial production fields as well as at Hyslop research farm in 1995. Three cultivars were included in the trial; Shademaster and Hector, which produce many rhizomes, and Seabreeze which produces few rhizomes. The effects of stubble height, PGRs, and field burning were measured during fall regrowth and flowering. Non-structural carbohydrates available for early regrowth were reduced when stubble was removed below 5.0 cm, particularly in first-year stands. Fall tiller height was increased by stubble remaining and was negatively correlated with flowering. Rhizome development was reduced when stubble was removed mechanically or burned to the crown, whereas yield potential was increased. Fall ethylene application reduced fall tiller height, fall tiller number, and percent fertile tillers the following spring and was similar to control treatment compared with burn. Other PGRs did not produce consistent results in this study. Excess ethylene produced by decaying stubble may impact floral induction and reduce yield potential in creeping red fescue seed crops. Light quality as measured by red:far-red ratio (R:FR) was reduced by canopy closure during regrowth but not by the presence of stubble. In controlled environment studies, red light (R) promoted taller tillers, greater stage of development, and greater tiller number than far-red (FR) light. Sunlight enriched with FR completely inhibited rhizome formation. Results suggest that environments with excess reflected FR may negatively impact early development of creeping red fescue seed crops. / Graduation date: 1998

Chewings fescue [Festuca rubra L. subsp. fallax (Thuill.) Nyman] is a desired turfgrass with dense sod forming capabilities and superior shade tolerance. Thermal residue management (open-field burning) has traditionally been used to remove post-harvest residue and maintain seed yield over the life of the stand. However, alternative non-thermal residue management practices have been observed to produce adequate seed yields dependent upon cultivar. Strong creeping red fescue (F. rubra L. subsp. rubra) is desired for its prolific tillering capacity and creeping rhizomatous growth habit. In contrast to Chewings fescue, maintenance of seed yield in strong creeping red fescue has only been profitably produced under thermal residue management. Slender creeping red fescue [F. rubra L. var. littoralis (Vasey)] is a desired turfgrass with a compact, less rhizomatous growth habit, similar to Chewings fescue in desirable turf attributes. However, little is known about the effects of post-harvest residue management in slender creeping red fescue. The objectives of this study were: 1) to evaluate seed yield and yield components among different cultivars to thermal (open-burning), and non-thermal (flail low and flail high) post-harvest residue management; 2) to evaluate harvest index and percent cleanout to thermal and non-thermal residue management in different cultivars; and 3) and to provide an economic analysis of thermal and non-thermal residue management in all cultivars based on partial budgeting. Three post-harvest residue management treatments (burn, flail low and flail high) were applied over the course of two years. Seed yield components measured included: total dry weight, fertile tiller number, spikelets per panicle, florets per spikelet, and panicle length. Final seed yield in each cultivar and residue management treatment method was determined after seed harvest and conditioning. Seed yield component analysis was conducted over three production seasons. Chewings fescue, strong creeping red fescue, and slender creeping red fescue cultivars responded differently to residue management as indicated by a residue management by cultivar interaction. In 2003 and 2004, residue management by cultivar interactions were evident in seed number, seed weight, fertile tiller number, percent cleanout, harvest index, and seed yield. Residue management by cultivar interactions occurred in spikelets per panicle in 2003, whereas in 2004 a residue management by cultivar interaction occurred in panicle length and florets per spikelet. In 2004, non-thermal flail low, and thermal residue management resulted in significantly greater spikelets per panicle in all cultivars. Thermal residue management resulted in the greatest number of spikelets per panicle. Results indicate that thermal residue management best maintained seed yield in most subspecies and cultivars across both years. However, in 2003, non-thermal flail low residue management produced profitable seed yield in only Marker slender creeping red fescue. In contrast, thermal residue management resulted in poor seed yields in Marker slender creeping red fescue and enhanced yields in Seabreeze slender creeping red fescue in 2003. However, following the second year of thermal treatment in 2004, Marker and Seabreeze both had lower seed yields, thus exhibiting the only negative impact of thermal management among the cultivars tested in this study. Moreover, upon review of an economic analysis, Marker slender red fescue was the only cultivar that produced a positive net return of $78 and $4 ha⁻¹ under non-thermal residue management in 2003 and 2004, respectively. Furthermore, in 2003, thermal residue management net return increases ranged from $104 ha⁻¹ to -$996 ha⁻¹ in Barnica and Shademark, respectively. In 2004, thermal residue management net return increases ranged from $115 ha⁻¹ and $1,332 ha⁻¹ in Seabreeze and Shademark, respectively. Poor seed yields were observed in all strong creeping red fescue cultivars under non-thermal residue management across both years of the study. This may be attributed to an observed reduction in fertile tiller number and seed yield. In addition, percent seed cleanout was increased with non-thermal residue management. In 2004, as stand age increased, thermal residue management resulted in greater seed yields in all cultivars and species, except both cultivars of slender creeping red fescue. Thus, this study provided substantial evidence that thermal residue management has the potential to maintain or increase fine fescue seed yield as stands age as well as to maintain stand profitability. / Graduation date: 2005

Open-field burning has been an effective, economical, and widespread method of post-harvest residue management in creeping red fescue seed production in the Willamette Valley since the late 1940s. However, the use of field burning has been legislatively restricted due to air quality and safety issues. The foliar-applied plant growth regulator trinexapac-ethyl (TE), commercialized in the USA as Palisade, has been accepted by producers as a yield enhancing agent and is considered here as an alternative to open-field burning over a four-year period. The effects of open-field burning versus mechanical removal (flailing) of post-harvest residue, and spring versus fall applications of TE on seed yield, dry matter partitioning, and seed yield components were evaluated in a split-plot design. The response to the different treatment combinations differed across years. The young stand responded with a seed yield increase to spring TE applications, regardless of residue management treatment. However, as the stand aged, field burning became critical for maintaining high yields and, in 2003 and 2004, only spring TE applications resulted in seed yield increases in burned plots. The higher potential seed yield achieved in burned plots over flailed plots, as a result of a higher number of panicles per unit area and spikelets per panicle, was critical for maintaining high seed yields as the stand aged. Spring applications of TE, further increased seed yield over the untreated check by increasing the number of florets per spikelet, reducing fertile tiller height and lodging and consequently, favoring pollination and fertilization of the florets. Late spring TE applications also increased 1000-seed weight in 2003 and 2004. Although spring applications of TE were a promising alternative to open-field burning early during the life of the stand, as the stand aged they did not increase seed yield on flailed plots. Fall TE applications did not have a consistent effect on seed yield, dry matter partitioning or seed yield components, and were found not to be a viable management practice. / Graduation date: 2005

The hypothesis for this study was that phenanthrene degrading bacterial inoculants, in combination with grass species able to tolerate petroleum hydrocarbon contamination, will result in increased degradation, as compared to natural rates of hydrocarbon degradation, or to rates of degradation attributed to bacteria or plants alone. Three experiments were performed to examine this hypothesis: i) assessment of the effect of phenanthrene degrading bacteria (<i>Sphingomonas yanoikuyae</i>, <i>Rahnella aquatilis</i>, and <i>Arthrobacter globiformis</i>) on seed germination, location of attachment on seeds and roots, and inoculant survival on selected grass species, ii) determination of the inoculant survival in contaminated soil in the absence of plants and the ability to degrade target compounds, and iii) degradation potential and survival of selected grass species and bacterial inoculants in soil. In general, all applied inoculants were able to effectively colonize the seeds and had a neutral or positive effect on seed germination and seedling growth. Possible plant and bacteria pairs were chosen based on positive influence of the inoculant and are as follows: perennial ryegrass (<i>Lolium perenne</i>) or creeping red fescue (<i>Festuca rubra</i>) with <i>A. globiformis</i> or <i>S. yanoikuyae</i>, or slender wheatgrass (<i>Elymus trachycaulus</i>) with <i>A. globiformis</i> or <i>R. aquatilis</i>. Soil-based assessment of the survival and degradation of hydrocarbons by the selected inoculants was examined with or without a manure nutrient amendment. The addition of the inoculants had a positive impact on the efficacy of hydrocarbon removal in the soil. The manure-amended soil, or <i>A. globiformis</i> inoculated non-amended soil treatments reduced total petroleum hydrocarbon concentration by ~45%, whereas the non-amended control only resulted in a ~20% reduction. When soils were amended with manure and inoculated with any of the phenanthrene degrading bacteria, contaminant concentration decreased in soil by ~33%. <i>Sphingomonas yanoikuyae</i> survived the longest in soil in the absence of plants. A growth chamber experiment was conducted to determine the efficacy of plant and bacteria pairs for hydrocarbon removal in recalcitrant contamination found in soil from Bruderheim, Alberta. Additional replicates containing this soil were spiked with hexadecane, phenanthrene, and pyrene so the effectiveness of the plant and bacteria pairs at higher levels of fresh contamination could be assessed. In the spiked treatment, inoculation with <i>S. yanoikuyae</i> increased creeping red fescue root biomass. In the non-spiked treatment, <i>S. yanoikuyae</i> application increased creeping red fescue root and shoot biomass. Perennial ryegrass root and shoot biomass did not increase when inoculated with <i>S. yanoikuyae</i>, although root biomass values were observably higher in non-spiked soils. Creeping red fescue inoculated with <i>S. yanoikuyae</i> resulted in the greatest decrease in hydrocarbon concentration as compared to other treatments (~61%). The perennial ryegrass treatment, when inoculated with <i>S. yanoikuyae</i> increased percent hydrocarbon removal (~10%) above that obtained with perennial ryegrass alone. The addition of plants and <i>S. yanoikuyae</i> increased hydrocarbon degradation relative to control soils, although the addition of vegetation alone had a comparable effect. A critical benefit of inoculation was the increase in creeping red fescue root biomass at higher concentrations of contamination. This is important because the larger the root biomass the larger the volume of soil that can be remediated. The results indicate that the use of specific plant-bacterial inoculants can enhance remediation of hydrocarbon contaminated soils.

The hypothesis for this study was that phenanthrene degrading bacterial inoculants, in combination with grass species able to tolerate petroleum hydrocarbon contamination, will result in increased degradation, as compared to natural rates of hydrocarbon degradation, or to rates of degradation attributed to bacteria or plants alone. Three experiments were performed to examine this hypothesis: i) assessment of the effect of phenanthrene degrading bacteria (<i>Sphingomonas yanoikuyae</i>, <i>Rahnella aquatilis</i>, and <i>Arthrobacter globiformis</i>) on seed germination, location of attachment on seeds and roots, and inoculant survival on selected grass species, ii) determination of the inoculant survival in contaminated soil in the absence of plants and the ability to degrade target compounds, and iii) degradation potential and survival of selected grass species and bacterial inoculants in soil. In general, all applied inoculants were able to effectively colonize the seeds and had a neutral or positive effect on seed germination and seedling growth. Possible plant and bacteria pairs were chosen based on positive influence of the inoculant and are as follows: perennial ryegrass (<i>Lolium perenne</i>) or creeping red fescue (<i>Festuca rubra</i>) with <i>A. globiformis</i> or <i>S. yanoikuyae</i>, or slender wheatgrass (<i>Elymus trachycaulus</i>) with <i>A. globiformis</i> or <i>R. aquatilis</i>. Soil-based assessment of the survival and degradation of hydrocarbons by the selected inoculants was examined with or without a manure nutrient amendment. The addition of the inoculants had a positive impact on the efficacy of hydrocarbon removal in the soil. The manure-amended soil, or <i>A. globiformis</i> inoculated non-amended soil treatments reduced total petroleum hydrocarbon concentration by ~45%, whereas the non-amended control only resulted in a ~20% reduction. When soils were amended with manure and inoculated with any of the phenanthrene degrading bacteria, contaminant concentration decreased in soil by ~33%. <i>Sphingomonas yanoikuyae</i> survived the longest in soil in the absence of plants. A growth chamber experiment was conducted to determine the efficacy of plant and bacteria pairs for hydrocarbon removal in recalcitrant contamination found in soil from Bruderheim, Alberta. Additional replicates containing this soil were spiked with hexadecane, phenanthrene, and pyrene so the effectiveness of the plant and bacteria pairs at higher levels of fresh contamination could be assessed. In the spiked treatment, inoculation with <i>S. yanoikuyae</i> increased creeping red fescue root biomass. In the non-spiked treatment, <i>S. yanoikuyae</i> application increased creeping red fescue root and shoot biomass. Perennial ryegrass root and shoot biomass did not increase when inoculated with <i>S. yanoikuyae</i>, although root biomass values were observably higher in non-spiked soils. Creeping red fescue inoculated with <i>S. yanoikuyae</i> resulted in the greatest decrease in hydrocarbon concentration as compared to other treatments (~61%). The perennial ryegrass treatment, when inoculated with <i>S. yanoikuyae</i> increased percent hydrocarbon removal (~10%) above that obtained with perennial ryegrass alone. The addition of plants and <i>S. yanoikuyae</i> increased hydrocarbon degradation relative to control soils, although the addition of vegetation alone had a comparable effect. A critical benefit of inoculation was the increase in creeping red fescue root biomass at higher concentrations of contamination. This is important because the larger the root biomass the larger the volume of soil that can be remediated. The results indicate that the use of specific plant-bacterial inoculants can enhance remediation of hydrocarbon contaminated soils.