The gastrocnemius muscle was removed from the hindlimb of female rodents, minced into 1mm3 fragments and autotransplanted. Experimental groups had the sural or tibial nerves laid in the mince as follows: both nerves (BN), sural only (SN), tibial only (TN) or no nerve (NN). Animals were sacrificed 45 days postsurgery and the regenerate and contralateral muscles studied for fiber composition and aerobic capacity. Regenerate mass and aerobic capacity were significantly less than control and followed the order BN > TN > SN > NN. The percentage of Types I, IIA and IIB fibers were also different between treatment groups. The data suggests (1) either a more rapid development of axons associated with IIA fibers following nerve transection or greater reinnervation capacity, and/or (2) a shift in the relative number of these axons in the regenerating nerve.

Thesis (Th. M.)--Detroit Baptist Theological Seminary, 2001. / Abstract. Includes bibliographical references (leaves 93-107).

Thesis (M.S.)--University of Louisville, 1995. / School of Dentistry, Department of Biological and Biophysical Sciences. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

Thesis (M. S.)--Biology, Georgia Institute of Technology, 2009. / Committee Chair: Boyan Barbara; Committee Member: Guldberg Robert; Committee Member: Lovachev Kiril; Committee Member: Schwartz Zvi. Part of the SMARTech Electronic Thesis and Dissertation Collection.

Thesis (Th. M.)--Detroit Baptist Theological Seminary, 2001. / Abstract. Includes bibliographical references (leaves 93-107).

Thesis (M.S.)--University of Louisville, 1995. / School of Dentistry, Department of Biological and Biophysical Sciences. Includes bibliographical references.

Thesis (Th. M.)--Detroit Baptist Theological Seminary, 2001. / Abstract. Includes bibliographical references (leaves 93-107).

Liver injury stimulates hepatocyte proliferation, regenerating the liver through self-replication. In cases where there is severe, repetitive, parenchymal damage, as seen in human chronic liver disease, hepatocyte mediated regeneration becomes impaired. In this setting it is currently unclear whether endogenous biliary epithelial cells can repopulate the hepatocyte compartment. This thesis therefore aimed to address this point by lineage tracing the main two liver epithelia populations on a background of impaired hepatocyte regeneration. To impair regeneration, an Itgb1 transgene was specifically deleted, conditionally, from the hepatocyte epithelium. Long-term loss of β1-Integrin alone or with additional injury caused an epithelial ductular reaction of biliary origin. Alongside β1-Integrin ablation, the hepatocyte epithelium was also labelled with a heritable ROSA26LSLtdTomato reporter. Impaired hepatocyte regeneration mediated by β1- integrin ablation resulted in 25% of hepatocytes becoming tdTomato negative (non-hepatocyte derived). To verify that the non-hepatocyte mediated regeneration was originating from the biliary epithelium, anti-Itgb1 RNAi was administrated to K19CreERT LSLtdTomato mice. Resulting in tdTomato positive hepatocytes that had differentiated from the labelled tdTomato positive biliary epithelial cells. In summary, this thesis demonstrates that hepatocyte β1-Integrin ablation combined with toxic damage causes marked ductular reactions and results in a substantial regeneration of functional hepatocytes from the biliary epithelium.

No description available.

published_or_final_version / Anatomy / Doctoral / Doctor of Philosophy