Sun protection has gained worldwide attention because repetitive overexposure to ultraviolet radiation can result in harmful effects on human skin, including sunburn, premature skin ageing, and in the worst case, skin cancer (Eckhardt and Rohwer, 2000; Sengupta and Blain, 2001). The diminishing stratospheric ozone layer, due to environmental degradation in the past few decades, combined with the modern outdoor-oriented lifestyles, are leading to unexpected levels of skin cancer (Davis, Capjack, Kerr, and Fedosejevs, 1997). Wearing Ultraviolet protective clothing is a simple way of practicing sun safety; however, regular cotton generally has very low ultraviolet protection and it is one of the most environmentally damaging crops despite of it is commonly used to make summer clothing. With the increased interest of public awareness related to sustainability and environmental issues, naturally colored cotton was recommended as it provides better ultraviolet protection than regular cotton. In addition, the production of naturally colored cotton is more environmentally friendly than regular cotton. Although several studies have been conducted on the UVR protection of naturally colored cotton, many questions regarding the factors that influence the UVR protection of fabrics remain unanswered. The primary purpose of the study was to examine the effects of perspiration application, weathering exposures, washing action of automatic home clothes washers, and repeated laundering on the UVR protection of a NC lightweight cotton fabric. In addition, five fabric property changes in the test specimen after the treatments of perspiration, weathering exposure, washing action, and repeated laundering (i.e., fabric count change, thickness change, weight change, color change and dimensional change) were included in this study to serve as secondary dependent variables to examine if the four treatment factors (i.e., perspiration application, weathering exposures, washing action of automatic home clothes washers, and repeated laundering) will cause changes in these five fabric properties, and if these changes will lead to changes of UVR protection of NC lightweight cotton fabric. Based on the purpose and objectives of the study, a split-plot repeated measures experimental design was used for the current study. In this study, the whole plot treatment was the weathering exposure, which contained three levels (i.e., semi-tropical climate without water spray, semi-arid climate, and standard conditioning), and the split plot treatments were the combinations of two treatment factors. In order to understand the effects of repeated laundering on the UVR protection and the five fabric properties, except for the control group, all test specimens were laundered after being treated with the three treatment factors (i.e., perspiration, weathering exposure, and washing action), and this process was repeated 15 times. The UVR protection (i.e., express in UPF value change in current study) and the five fabric properties of these treated test specimens were measured before laundering, and after each laundering cycle. The results of UPF value change showed that test specimens treated with perspiration had a lower change in UPF value than the specimens without treatment. The test specimens exposed to Florida condition had the most UPF value change, followed by Arizona and Standard textile testing conditions. A significant difference also found in test specimens that laundered in a traditional washer after ninth cycle and the UPF value decreased as the number of laundering cycle increased. However, test specimens that laundered in a front-loading HE washer showed no significant UPF value change. For the five fabric properties that listed in secondary objective, all four treatments significantly influenced fabric count, fabric thickness and fabric weight. However, perspiration treatment had no significant effect on the dimensional change in warp direction of test specimens, and washing action had no significant effect on the dimensional change in filling direction of the test specimen as well as both Delta E and Delta L of color change. For testing the relationship between the changes of the five fabric properties and UPF value change, Delta E and Delta L of color change had the highest correlation coefficient with UPF value change. Therefore, it is possible that the changes of these two properties caused by the four treatments and lead to the UPF value change. Future research is needed to confirm this relationship. In conclusion, of perspiration application, weathering exposures, washing action of automatic home clothes washers, and repeated laundering do have influence on the ultraviolet protection of the naturally colored cotton. The color change of the test specimens caused by these four treatments possible lead to the change of the ultraviolet protection of the test specimens. More studies are needed to confirm this relationship. / Ph. D.