The purpose of this study was to perform an indoor air quality (IAQ) investigation at the Center for Applied Reproductive Science (CARS) to assess whether VOCs exist at levels dangerous to embryo. Formaldehyde, n-hexane, benzene, and styrene concentrations were measured at six locations. Formaldehyde concentrations were comparable to office and residential indoor air. N-hexane, benzene, and styrene were not detected. In addition, acetaldehyde, ethanol, and isopropyl alcohol were detected. IAQ parameters (carbon dioxide, temperature, humidity, pressure, and particulates) were measured at 22 sites monthly for one year. Temperature and humidity readings were within Environmental Protection Agency recommendations. Particulate concentrations were below Occupational Safety and Health Administration standards. Pressure readings indicated the facility was under a negative pressure. Carbon dioxide concentrations exceeded recommendations established by American Society for Heating, Refrigeration, and Air Conditioning Engineers (ASHRAE). Recommendations include assessing air intakes to assure dampers are adjusted to allow 15 ft3/min/person of fresh air established by ASHRAE.