Submitted by PPG Odontologia (odontologia-pg@pucrs.br) on 2018-03-09T13:09:24Z No. of bitstreams: 1 FERNANDA_BORGUETTI_MANFROI_TES.pdf: 2715505 bytes, checksum: 81352bd5d757e75e046659bb79849f86 (MD5) / Approved for entry into archive by Tatiana Lopes (tatiana.lopes@pucrs.br) on 2018-03-12T12:58:50Z (GMT) No. of bitstreams: 1 FERNANDA_BORGUETTI_MANFROI_TES.pdf: 2715505 bytes, checksum: 81352bd5d757e75e046659bb79849f86 (MD5) / Made available in DSpace on 2018-03-12T13:06:37Z (GMT). No. of bitstreams: 1 FERNANDA_BORGUETTI_MANFROI_TES.pdf: 2715505 bytes, checksum: 81352bd5d757e75e046659bb79849f86 (MD5) Previous issue date: 2018-01-16 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / (Artigo 1 - Efeito dos ciclos de pH na resist?ncia de uni?o de diferentes sistemas adesivos ? dentina) Purpose: To verify the influence of the pH cycles on the microtensile bond strength (?TBS) to dentin of three adhesive systems compared to the immediate ?TBS and after storage in artificial saliva for 6 months. Materials and methods: The occlusal surface of 32 sound third molar teeth was removed to obtain a flat dentin surface. The teeth were randomly divided into four groups (n=8/group): G1 - Single Bond Universal in the total-etch mode; G2 ? Single Bond Universal in the self-etch mode; G3 ? Scotchbond Multi Purpose (totaletch mode control); G4 ? Clearfil SE Bond (self-etch mode control). A block of composite with 6 mm height was built on the adhesive area. After 24 h of storage in distilled water at 37?C, the tooth/resin sets were cut parallel to the long axis of the tooth, in the x and y directions, with a section of approximately 0.8 mm2 . Fifty-two specimens were obtained for each group, of which 26 were submitted to pH cycles and the other 26 were stored in artificial saliva for 6 months. Subsequently, the specimens were submitted to a ?TBS test in a universal testing machine at a crosshead speed of 0.5 mm/min. For the immediate evaluation (24 h), results previously obtained were used for this study. Results: According to the two-way ANOVA test, the interaction between the factors (p=0.0001) was significant. There was no significant difference in the immediate ?TBS between the adhesive systems. After 4 cycles of pH and 6 months of storage in artificial saliva, there was a significant decrease in ?TBS for all adhesive systems. The failures were predominantly mixed. Conclusion: The pH cycles and the storage in artificial saliva for 6 months were detrimental to the stability of the bonding interface. (Artigo 2 - Efeito dos ciclos de pH na nanoinfiltra??o de diferentes sistemas adesivos ? dentina) Purpose: To verify the influence of the pH cycles on the nanoleakage in the dentin-adhesive interface of three adhesive systems compared to the immediate nanoleakage and after storage in artificial saliva for 6 months. Materials and methods: The occlusal surface of 24 sound third molar teeth was removed to obtain a flat dentin surface. The teeth were randomly divided into four groups (n=6/group): G1 ? Single Bond Universal in the total-etch mode; G2 ? Single Bond Universal in the self-etch mode; G3 ? Scotchbond Multi Purpose (total-etch mode control); G4 ? Clearfil SE Bond (self-etch mode control). A block of composite with 6 mm height was built on the adhesive area. After 24 h of storage in distilled water at 37?C, the tooth/resin sets were cut parallel to the long axis of the tooth to obtain slices with a thickness of approximately 1.0 mm. Three slices of the central region of each tooth were obtained. One slice was submitted to pH cycles, and one slice was stored in artificial saliva for 6 months. The nanoleakage methodology was applied. Images of the dentin-adhesive interfaces were obtained by scanning electron microscopy (2000x), and the images were analyzed in the ImageJ program to evaluate the percentage of nanoleakage area (%N). Results: According to the two-way ANOVA test, the interaction between the factors (adhesive system x storage) (p=0.0001) was significant. There was no significant difference in the %N between the adhesive systems at 24 h. After the pH cycles, the adhesive systems applied in the self-etch mode obtained lower %N, differing significantly from total-etch adhesives. After 6 months in artificial saliva, there was lower %N compared to pH cycles, and the lowest %N occurred in self-etch adhesives. Conclusion: The pH cycles caused higher nanoleakage than storage in artificial saliva for 6 months. The self-etch adhesive systems had lower nanoleakage after pH cycles and storage in artificial saliva for 6 months in relation to total-etch adhesive systems. / (Artigo 1 - Efeito dos ciclos de pH na resist?ncia de uni?o de diferentes sistemas adesivos ? dentina) Objetivo: Verificar a influ?ncia dos ciclos de pH nos valores de resist?ncia de uni?o ? microtra??o (R?T) ? dentina de tr?s sistemas adesivos em compara??o com a R?T imediata e ap?s armazenamento em saliva artificial pelo per?odo de 6 meses. Materiais e m?todos: A superf?cie oclusal de 32 dentes terceiros molares h?gidos foi removida para obter superf?cie plana em dentina, sendo divididos aleatoriamente em quatro grupos (n=8/grupo): G1 ? Single Bond Universal na vers?o total-etch; G2 ? Single Bond Universal na vers?o self-etch; G3 ? Scotchbond Multi Purpose (controle da vers?o total-etch); G4 ? Clearfil SE Bond (controle da vers?o self-etch). Sobre o sistema adesivo foi confeccionado um bloco de 6 mm de altura em resina composta. Ap?s 24 h de armazenagem em ?gua destilada a 37?C, os conjuntos dente/resina foram cortados paralelamente no longo eixo do dente, nos sentidos x e y, com sec??o de aproximadamente 0,8 mm2 . Cinquenta e dois corpos de prova foram obtidos, em m?dia, para cada grupo, sendo 26 submetidos aos ciclos de pH, e os outros 26 ao armazenamento em saliva artificial por 6 meses. Na sequ?ncia, os corpos de prova foram submetidos ao teste de microtra??o em m?quina de ensaio universal EMIC DL-2000, com uma velocidade de 0,5 mm/min. Os tipos de falha foram observados em microscopia eletr?nica de varredura. Para a avalia??o imediata (24 h) foram utilizados resultados obtidos previamente a este estudo. Resultados: De acordo com o teste ANOVA de duas vias a intera??o entre os fatores (p=0,0001) foi significativa. N?o houve diferen?a estat?stica na R?T imediata entre os sistemas adesivos. Ap?s os 4 ciclos de pH e 6 meses de armazenamento em saliva artificial houve redu??o significativa das m?dias de R?T para todos os sistemas adesivos. As falhas foram predominantemente mistas. Conclus?o: Os ciclos de pH e o armazenamento em saliva artificial durante 6 meses prejudicaram a estabilidade da interface de uni?o. (Artigo 2 - Efeito dos ciclos de pH na nanoinfiltra??o de diferentes sistemas adesivos ? dentina) Objetivo: Verificar a influ?ncia dos ciclos de pH na nanoinfiltra??o da interface dentina-adesivo de tr?s sistemas adesivos em compara??o com a nanoinfiltra??o imediata e ap?s armazenamento em saliva artificial por 6 meses. Materiais e m?todos: A superf?cie oclusal de 24 dentes terceiros molares h?gidos foi removida para obter superf?cie plana em dentina, sendo divididos aleatoriamente em quatro grupos (n=6/grupo): G1 ?Single Bond Universal na vers?o total-etch; G2 ? Single Bond Universal na vers?o self-etch; G3 ? Scotchbond Multi Purpose (controle da vers?o total-etch); G4 ? Clearfil SE Bond (controle da vers?o self-etch). Sobre o sistema adesivo foi confeccionado um bloco de 6 mm de altura em resina composta. Ap?s 24 h de armazenagem em ?gua destilada a 37?C, os conjuntos dente/resina foram cortados paralelamente no longo eixo do dente, resultando em fatias, com espessura de aproximadamente 1,0 mm. Tr?s fatias da regi?o central de cada dente foram obtidas, sendo uma fatia submetida aos ciclos de pH, e uma fatia armazenada em saliva artificial por 6 meses. Na sequ?ncia foi aplicada a metodologia de nanoinfiltra??o. Imagens das interfaces dentina-adesivo foram obtidas por meio de microscopia eletr?nica de varredura (2000x), sendo as imagens analisadas no programa ImageJ para avaliar a porcentagem de ?rea de nanoinfiltra??o (%N). Resultados: De acordo com o teste ANOVA de duas vias, a intera??o dos fatores (sistema adesivo x armazenamento) foi significativa (p=0,0001). N?o houve diferen?a estat?stica na %N entre os sistemas adesivos na avalia??o em 24 h. Ap?s os ciclos de pH os sistemas adesivos aplicados de maneira self-etch obtiveram menor %N, diferindo estatisticamente dos adesivos total-etch. Ap?s 6 meses em saliva artificial, houve menor %N comparado aos ciclos de pH, e as menores %N ocorreram nos adesivos self-etch. Conclus?o: Os ciclos de pH causaram maior nanoinfiltra??o do que o armazenamento em saliva artificial por 6 meses. Os sistemas adesivos aplicados de maneira selfetch tiveram menor nanoinfiltra??o ap?s os ciclos de pH e armazenamento em saliva artificial por 6 meses em rela??o aos sistemas adesivos total-etch.

Submitted by PPG Odontologia (odontologia-pg@pucrs.br) on 2018-05-28T13:41:25Z No. of bitstreams: 1 RAQUEL_DE_OLIVEIRA_LOPES_TES.pdf: 2230148 bytes, checksum: a749d045cae0f8098669b38fe6b648b6 (MD5) / Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2018-06-11T18:19:23Z (GMT) No. of bitstreams: 1 RAQUEL_DE_OLIVEIRA_LOPES_TES.pdf: 2230148 bytes, checksum: a749d045cae0f8098669b38fe6b648b6 (MD5) / Made available in DSpace on 2018-06-11T18:25:33Z (GMT). No. of bitstreams: 1 RAQUEL_DE_OLIVEIRA_LOPES_TES.pdf: 2230148 bytes, checksum: a749d045cae0f8098669b38fe6b648b6 (MD5) Previous issue date: 2018-01-17 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / (Artigo Os adesivos universais s?o t?o eficientes quanto um primer para a ades?o ? zirc?nia?) O objetivo do trabalho foi comparar a resist?ncia de uni?o ? cer?mica de zirc?nia de quatro adesivos universais e um primer para zirc?nia. Setenta e cinco amostras de zirc?nia foram confeccionadas e inclu?das em resina acr?lica. A superf?cie das amostras foi polida com lixas de carbeto de sil?cio nas granula??es 600, 800 e 1.200 e jateadas com ?xido de alum?nio 50 ?m por 5 s. As amostras foram divididas aleatoriamente em cinco grupos (n=15): G1 ? Single Bond Universal (SBU); G2 ? All Bond Universal (ABU); G3 ? Peak Universal Bond (PUB); G4 ? Ambar Universal (AU) e G5 ? Z-Prime Plus (ZP). Um cone de resina composta foi constru?do, atrav?s de uma matriz, sobre o material adesivo aplicado na superf?cie das amostras. Os corpos de prova foram armazenados em ?gua destilada a 37?C por 24 h, sendo submetidos ao teste de resist?ncia de uni?o ? tra??o em m?quina de ensaio universal EMIC com velocidade de 0,5 mm/min. Os tipos de falhas foram classificados em adesiva, coesiva ou mista. Os valores de resist?ncia de uni?o foram submetidos ? an?lise de vari?ncia (ANOVA), seguido do teste de Tukey (?=0,05). M?dias de resist?ncia de uni?o (MPa) seguidas de letras distintas diferem estatisticamente entre si: G5=21,12a, G1=20,55a, G4=19,12ab, G2=14,22b, G3=8,45c. As falhas foram predominantemente mistas no G1, G4 e G5, e predominantemente adesivas no G2 e G3. Os adesivos SBU e AU obtiveram resist?ncia de uni?o compar?vel ao ZP. / (Artigo Bond to zirconia ceramic: evaluation of different primers and a universal adhesive) The aim of the study was to evaluate the effect of a universal adhesive and different primers on the bond strength to zirconia ceramic. Seventy-five zirconia ceramic samples were obtained and divided into five groups (n=15): G1 ? Scothbond Universal (SBU); G2 ? silane + SBU; G3 - Signum Zirconia Bond; G4 - Z-Prime Plus; G5 - MZ Primer. A cone of composite resin was built. The specimens were stored in 100% relative humidity with distilled water at 37?C for 48 h, and then submitted to a tensile bond strength test in a universal testing machine at a crosshead speed of 0.5 mm/min. The type of failure that occurred during the debonding procedure was analyzed. The mean results of the bond strength test (MPa) followed by the same letter represent no statistical difference by ANOVA and Tukey?s post-hoc test (p<0.05): G2=27.55a (?6.99), G4=23.71a (?5.65), G1=22.64a (?5.67), G5=13.64b (?5.49), G3=7.54c (?4.75). G2 and G4 exhibited predominantly cohesive failure in the resin composite cone. G1 and G5 had predominantly mixed failures, and G3 exhibited only adhesive failures. The SBU and Z-Prime Plus provided higher bond strength to zirconia ceramic. / (Artigo Os adesivos universais s?o t?o eficientes quanto um primer para a ades?o ? zirc?nia?) The aim of the study was to compare four universal adhesives and a primer on the bond strength to zirconia ceramic. Seventy-five zirconia ceramic samples were obtained and embedded in acrylic resin. The surface of the samples was polished with 600-, 800- and 1200-grit silicon carbide abrasive papers, and sandblasted with 50 ?m aluminum oxide particles for 5 s. The samples were divided into five groups (n=15): Single Bond Universal (SBU); G2 ? All Bond Universal (ABU); G3 ? Peak Universal Bond (PUB); G4 ? Ambar Universal (AU) e G5 ? Z-Prime Plus (ZP). A cone of composite resin was built on the adhesives and primer applied. The specimens were stored in distilled water at 37oC for 24 h, and then submitted to a tensile bond strength test in a universal testing machine at a crosshead speed of 0.5 mm/min. The type of failure that occurred during the debonding procedure was classified as adhesive, cohesive or mixed. The values of bond strength were analyzed by analysis of variance (ANOVA) followed by Tukey?s test (?=0.05). Means of bond strength (MPa) followed by the distinct letters represent statistical difference: G5=21,12a, G1=20,55a, G4=19,12ab, G2=14,22b, G3=8,45c. The failures were predominantly mixed in G1, G4 and G5, and predominantly adhesive in G2 and G3. SBU and AU adhesives obtained bond strength comparable to ZP.

Submitted by SBI Biblioteca Digital (sbi.bibliotecadigital@puc-campinas.edu.br) on 2017-02-21T18:17:11Z No. of bitstreams: 1 Diana Roberta Pereira Grandizoli.pdf: 22186247 bytes, checksum: eda73530f877f7998ed73ea7c0033e18 (MD5) / Made available in DSpace on 2017-02-21T18:17:11Z (GMT). No. of bitstreams: 1 Diana Roberta Pereira Grandizoli.pdf: 22186247 bytes, checksum: eda73530f877f7998ed73ea7c0033e18 (MD5) Previous issue date: 2016-12-19 / The present study sought to evaluate the influence of protease inhibitors on the bond strength of a self-etch adhesive system during hybrid layer formation in caries-affected dentin. The occlusal thirds of 80 permanent third molars were ground down to flat dentin surfaces. Dentinal caries were induced artificially by the microbial method. Groups were divided as follows: G1 (n=20), application of Clearfil SE Bond adhesive system (CL) alone; G2 (n=20), pretreatment with 2% chlorhexidine (CLX)+CL; G3 (n=20), pretreatment with sodium bicarbonate (BIC)+CL; G4 (n = 20), BI +CLX+CL. Bond strength was assessed immediately and at 6 months. Composite resin (Z350) build-ups were made on the dentin surfaces, and beam-shaped specimens with a cross-sectional area of 1 mm? were obtained. Microtensile bond strength testing was performed. Only adhesive or mixed-mode fractures were taken into account for calculation of bond strength. The results were submitted to the Kruskal-Wallis test (Student-Newman-Keuls). There was no significant difference on bond strength between the control, bicarbonate and chlorhexidine groups in the immediate test (p> 0.05). After 6 months, adhesive resistance fell for all groups. The control group had higher bond strength (p <0.05). The predominant fracture was of the adhesive type independent of the period evaluated. It can be concluded that after six months, there was decrease on bond strength for all groups. This reduction was greater in the groups in which the inhibitors were used / O objetivo desse trabalho foi avaliar a influ?ncia do bicarbonato de s?dio na resist?ncia adesiva durante a hibridiza??o da dentina cariada utilizando o sistema adesivo autocondicionante. Foram selecionados 80 terceiros molares permanentes, feita a remo??o do ter?o oclusal e superf?cies dentin?rias planas foram obtidas. A les?o de c?rie dentin?ria foi confeccionada atrav?s do m?todo microbiol?gico. Os grupos foram: G1 (n=20): aplica??o do sistema adesivo Clearfil (CL), G2 (n=20): aplica??o de clorexidina 2% (CLX) + CL, G3 (n=20): aplica??o de bicarbonato de s?dio (BI) + CL, G4 (n=20): BI + CLX + CL. A resist?ncia adesiva foi avaliada imediatamente e ap?s seis meses. Um bloco de resina composta Z350 foi constru?do em dentina. Cada bloco dente/adesivo/resina foi seccionado com disco diamantado acoplado ? m?quina de corte em planos paralelos obtendo-se corpos de prova em forma de palito, com ?rea de sec??o transversal de 1 mm?. Foi realizado teste de resist?ncia de uni?o por meio do teste de microtra??o. Foi considerada apenas fratura adesiva/mista para c?lculo da resist?ncia de uni?o. Os resultados foram submetidos aos teste de Kruskal-Wallis (Student-Newman-Keuls). N?o houve diferen?a significante na resist?ncia de uni?o entre os grupos controle, bicarbonato e clorexidina no teste imediato (p>0.05). Ap?s 6 meses, houve queda da resist?ncia adesiva para todos os grupos. O grupo controle apresentou maior resist?ncia de uni?o (p<0.05). A fratura predominante foi do tipo adesiva independente do per?odo avaliado. Pode-se concluir que ap?s seis meses, houve diminui??o na for?a de uni?o para todos os grupos. Essa redu??o apresentou-se maior nos grupos em que os inibidores foram utilizados.