Thesis (Ph. D.)--University of Wisconsin--Madison, 1983. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.

by Leung Sau-mei. / With: Two articles bound together subsequent to publication. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1990. / Bibliography: leaves 101-108. / Abstract --- p.i / Acknowledgements --- p.iv / List of Abbreviations --- p.v / Table of content --- p.vi / Chapter Section 1 --- Introduction / Chapter 1.1 --- The phenomenon of host controlled restriction and modification in bacteria --- p.2 / Chapter 1.2 --- Classification of restriction and modification systems --- p.3 / Chapter 1.3 --- The nomenclature system for restriction and modification systems --- p.8 / Chapter 1.4 --- Variety of type II restriction and modification systems --- p.9 / Chapter 1.5 --- Properties of type II restriction endonucleases --- p.11 / Chapter 1.5.1 --- Biological function --- p.11 / Chapter 1.5.2 --- Protein structure --- p.12 / Chapter 1.5.3 --- Genetics --- p.16 / Chapter 1.5.4 --- Cleavage mechanism --- p.18 / Chapter 1.5.5 --- Factors affecting optimal activity --- p.21 / Chapter 1.6 --- Aim of study --- p.27 / Chapter Section 2 --- Materials and methods / Chapter 2.1 --- Screening for type II restriction endonucleases --- p.28 / Chapter 2.1.1 --- Sources --- p.28 / Chapter 2.1.2 --- Preparation of crude enzyme extract --- p.29 / Chapter 2.1.3 --- Assay of restriction enzyme activity --- p.30 / Chapter 2.1.4 --- Characterization of strains --- p.31 / Chapter 2.2 --- Purification of restriction endonucleases --- p.31 / Chapter 2.2.1 --- Preparation of column materials --- p.32 / Chapter 2.2.2 --- Purification of BcoI --- p.33 / Chapter 2.2.3 --- "Purification of Bcol0278I, BspI, Bsu8646I and PvuHKU" --- p.33 / Chapter 2.2.4 --- Purification of Bsu8565I and Pei9403I --- p.33 / Chapter 2.2.5 --- Purification of Sol3335I --- p.34 / Chapter 2.3 --- Characterization of discovered restriction endonucleases --- p.34 / Chapter 2.3.1 --- Determination of recognition specificity --- p.34 / Chapter 2.3.2 --- Determination of cleavage specificity of BcoI --- p.35 / Chapter 2.3.3 --- Unit definition --- p.36 / Chapter 2.3.4 --- Assays for ionic requirement and optimal temperature --- p.37 / Chapter 2.3.5 --- Heat inactivation --- p.37 / Chapter 2.4 --- Construction of Bacillus coagulans SM1 genomic library --- p.38 / Chapter 2.4.1 --- Preparation of chromosomal DNA --- p.38 / Chapter 2.4.1.1 --- Restriction digestion of chromosomal DNA --- p.40 / Chapter 2.4.2 --- Large scale preparation of vector pBR322 DNA --- p.43 / Chapter 2.4.2.1 --- Restriction digestion of vector DNA --- p.44 / Chapter 2.4.2.2 --- Preparation of lambda insert DNA for control tests --- p.45 / Chapter 2.4.3 --- Ligation of insert and vector DNA --- p.46 / Chapter 2.4.4 --- Transformation --- p.46 / Chapter 2.4.4.1 --- Preparation of electro-competent cells --- p.46 / Chapter 2.4.4.2 --- Electro-transformation --- p.47 / Chapter 2.4.5 --- Rapid screening for the presence of plasmid --- p.49 / Chapter Section 3 --- Results / Chapter 3.1 --- Presence of type II restriction endonucleases --- p.50 / Chapter 3.2 --- Strain identification --- p.50 / Chapter 3.3 --- Purification and properties of the discovered restriction endonucleases --- p.52 / Chapter 3.3.1 --- BcoI --- p.55 / Chapter 3.3.2 --- "Bco10278I, BspI, Bsu8646I and PvuHKUI" --- p.59 / Chapter 3.3.3 --- Bsu8565I and Pei9403I --- p.65 / Chapter 3.3.4 --- Sol3335I --- p.70 / Chapter 3.4 --- Construction of Bacillus coagulans SM1 genomic library --- p.73 / Chapter 3.4.1 --- Preparation of chromosomal DNA --- p.73 / Chapter 3.4.1.1 --- Generation of 4-10 kb insert fragments --- p.73 / Chapter 3.4.2 --- Preparation of plasmid DNA --- p.75 / Chapter 3.4.3 --- Ligation of insert and vector DNA --- p.76 / Chapter 3.4.4 --- Rapid screening for the presence of plasmid --- p.77 / Chapter Section 4 --- Discussion / Chapter 4.1 --- Screening of type II restriction endonucleases --- p.79 / Chapter 4.1.1 --- Methods for screening of type II restriction endonucleases --- p.79 / Chapter 4.1.2 --- Factors affecting the detection of restriction endonucleases --- p.83 / Chapter 4.2 --- Purification of the discovered restriction endonucleases --- p.87 / Chapter 4.3 --- Characterization of discovered restriction endonucleases --- p.91 / Chapter 4.3.1 --- Determination of recognition site --- p.91 / Chapter 4.3.2 --- Determination of cleavage specificity --- p.93 / Chapter 4.4 --- Characteristics of the discovered restriction endonucleases --- p.95 / Chapter 4.5 --- Molecular cloning of BcoI --- p.96 / Chapter 4.6 --- Future prospects --- p.99 / References --- p.101 / Appendix I --- p.109

Thesis (Ph. D.)--University of Washington, 2000. / Vita. Includes bibliographical references (leaves 81-84).

No description available.

Thesis (Ph. D.)--University of Washington, 1989. / Vita. Includes bibliographical references (leaves [83]-90).

This study provides information about the effects of polyamines on the restriction enzymatic cleavage of bacteriophage lambda DNA. The polyamines studied were spermine, spermidine, Nl-acetylspermidine and N8- acetylspermidine. The restriction enzymes studied were Xhoi, BamHI, EcoRI, and Hindiii. The electrophoretic pattern of lambda DNA digests by these enzymes were recorded photographically. These results were further analyzed by spectrographic digitization and replotting. Polyamines affect the electrophoretic pattern of restriction fragments in two ways: by causing DNA streaking and by decreasing ethidium bromide binding to DNA, which in turn affects DNA staining properties. The polyamines studied have effects which are increasingly dependent on the charge of the polyamine. The concentration necssary to alter the electrophoretic pattern decreases with increased positive charge of the polyamines. Spermine, the most highly charged polyamine studied, resulted in alterations at a lower concentration than any other polyamines studied. Following spermine was spermidine, and then the two acetylated polyamines, Nl-acetylspermidine, and N8- acetylspermidine.

Thesis (Ph. D.)--University of Washington, 2002. / Vita. Includes bibliographical references (leaves 95-109).

Thesis (Ph. D.)--University of Missouri-Columbia, 1998. / Typescript. Vita. Includes bibliographical references (leaves 205-207). Also available on the Internet.

Thesis (Ph. D.)--University of Missouri-Columbia, 1998. / Typescript. Vita. Includes bibliographical references (leaves 77-85). Also available on the Internet.

Thesis (Ph. D.)--University of Missouri-Columbia, 1998. / Typescript. Vita. Includes bibliographical references (leaves 205-207). Also available on the Internet.