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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Desenvolvimento de revestimento à base de concentrado protÃico de soro de leite e Ãleo essencial de erva doce e sua eficiÃncia na vida pÃs-colheita de mamÃo "Golden" / Protein concentrate based coating development whey and essential oil of fennel and its efficiency in papaya postharvest life " Golden "

Jorgiane da Silva Severino Lima 10 July 2015 (has links)
Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico / O mamÃo (Carica papaya L.) à um fruto climatÃrico, apresenta altas taxas de transpiraÃÃo e, devido ao intenso metabolismo durante o amadurecimento, senesce rapidamente, dificultando o armazenamento, por perÃodos prolongados e, consequentemente gerando altos Ãndices de perdas pÃs-colheita, as quais podem ser agravadas pela aÃÃo do Colletotrichum gloeosporioides. Na perspectiva de estender o tempo de vida Ãtil do mamÃo ÂGolden este estudo objetivou desenvolver um revestimento usando uma combinaÃÃo de concentrado protÃico de soro de leite (CPSL), Ãleo essencial de erva doce (OED), cloreto de cÃlcio (CC) e glicerol (G).Foram realizadas avaliaÃÃes in vitro do efeito de diferentes concentraÃÃes de OED na reduÃÃo do crescimento micelial de C. gloesporioides, assim como foram testados in vitro a capacidade fungitÃxica do OED. Utilizando as duas concentraÃÃes mais eficientes do OED juntamente com diferentes concentraÃÃes de CPSL (10, 12, 14%), CC (1%) e G (5%), foram formulados nove revestimentos, os quais tiveram sua estabilidade avaliada por meio das anÃlises de Ãngulo de contato, diÃmetro mÃdio das partÃculas, potencial zeta e microscopia de varredura. De posse dos revestimentos mais promissores, foram iniciados trÃs experimentos em paralelo. No primeiro,mamÃes inoculados artificialmente com C. gloesporiodes foram submetidos aos revestimentos,para avaliaÃÃo da incidÃncia e severidade do patÃgeno. No segundo e terceiro experimentos, mamÃes sadios foram revestidos e submetidos à anÃlise de sobrevivÃncia e da qualidade, respectivamente. Os testes in vitro demonstraram um potencial positivo na utilizaÃÃo do Ãleo de erva doce no desenvolvimento micelial do fungo C. gloesporioides, com concentraÃÃo mÃnima inibitÃria (CMI) e concentraÃÃo mÃnima fungicida de 2000ppm. O conjunto de analises utilizado para avaliar a estabilidade dos revestimentos formulados indicou que, aqueles contendo menor percentual de CPSL (10%)e maiores concentraÃÃes de OED (0,2% e 0,4%) proporcionaram, a formaÃÃo de revestimentos mais estÃveis. No experimento utilizando mamÃes inoculados com C. gloeosporioides, foi possÃvel observar menor incidÃncia e severidade da doenÃa nos pontos nÃo inoculados dos mamÃes revestidos. Os revestimentos testados na anÃlise de sobrevivÃncia foram capazes de retardar o processo de maturaÃÃo dos mamÃes, sendo o tratamento composto por: 10% de CPSL, 0,4% de OED, 1% de CC e 5% de G o mais eficiente no controle da incidÃncia de C. gloeosporioides e na extensÃo da vida pÃs-colheita destes frutos. / Papaya (Carica papaya L.) is a climacteric fruit, has high transpiration rates and due to the intense metabolism during ripening, senesce quickly, making it difficult to store for long periods and consequently generating high rates of post harvest losses, which may be aggravated by the action of Colletotrichum gloeosporioides. In view of extending the useful life of the papaya'Golden' this study aimed to develop a coating using a combination of protein whey concentrate (WPC), essential oil of fennel (OED), calcium chloride (CC) and glycerol (G). Evaluations were carried out in vitro effect of different concentrations of OED reduction in mycelial growth of C. gloesporioides as were tested in vitro fungitoxic capacity of the O ED. Using the two most efficient concentrations of OED togethe r with different concentrations of WPC (10, 12, 14%), DC (1%) and G (5%) were formulated nine coatings, which have had their stability evaluated using the analysis contact angle, average particle diameter, zeta potential and microscopy. Possession of the most promising coatings were started three experiments in parallel. In the first, papayas artificially inoculated with C. gloesporiodes were submitted to coatings, to assess the incidence and severity of the pathogen. The second and third experiments, sound papayas were coated and subjected to analysis of survival and quality respectively. In vitro tests showed a positive potential in use of the fennel oil on mycelial growth of the fungus C. gloesporioides with minimal inhibitory concentration (MIC) and mini mum fungicidal concentration of 2000 ppm. The set of analyzes used to assess the stability of the formulated coatings indicated that those containing a lower percentage of WPC (10%) and the OED higher concentrations (0.2% and 0.4%) provided the formation of more stable coatings. In the experiment using papayas inoculated with C. gloeosporioides, we observed a lower incidence and severity of disease in non - inoculated points of the coated papayas Coatings tested in survival analysis were able to slow the ripening process of papayas, and the treatment consists of: 10% of CPSL, 0.4% O ED, 1% and 5% CC G was the most effective in controlling the incidence of C. gloeosporioides and the extension of postharvest life of these fruits

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