by Xu, Jing Huai. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1994. / Includes bibliographical references (leaves 119-126). / ABSTRACT --- p.i / ACKNOWLEDGMENTS --- p.ii / DEDICATION --- p.iii / TABLE OF CONTENTS --- p.iv / LIST OF ABBREVIATIONS --- p.viii / LIST OF FIGURES --- p.ix / LIST OF TABLES --- p.xii / Chapter 1. --- Introduction / Chapter 1.1. --- General Introduction --- p.1 / Chapter 1.2. --- Purpose of Study --- p.2 / Chapter 1.3. --- General Consideration and Objectives --- p.3 / Chapter 2. --- Literature Review / Chapter 2.1. --- Rhododendrons in Hong Kong --- p.9 / Chapter 2.2. --- History of Tissue Culture --- p.23 / Chapter 2.3. --- Tissue Culture of Rhododendron --- p.26 / Chapter 3. --- Materials and Methods / Chapter 3.1 --- General Protocol --- p.31 / Chapter 3.2. --- Plant Materials --- p.31 / Chapter 3.3. --- Culture Media / Chapter 3.3.1. --- Basal medium --- p.32 / Chapter 3.3.2. --- Sterilization --- p.32 / Chapter 3.4. --- Growth Condition --- p.33 / Chapter 3.5. --- Establishment of the Aseptic Culture / Chapter 3.5.1. --- Explants --- p.33 / Chapter 3.5.2. --- Pretreatment --- p.33 / Chapter 3.5.3. --- Disinfection --- p.33 / Chapter 3.5.4. --- Prevention of Browning --- p.34 / Chapter 3.6. --- Multiplication of the Culture / Chapter 3.6.1. --- General Operation --- p.35 / Chapter 3.6.2. --- Basal Medium --- p.35 / Chapter 3.6.3. --- Combination of Various Plant Hormones --- p.36 / Chapter 3.6.4. --- Addition of Vitamins --- p.36 / Chapter 3.6.5. --- Filter Paper as Supporting Material --- p.36 / Chapter 3.7. --- In Vitro Rooting / Chapter 3.7.1. --- General Operation --- p.37 / Chapter 3.7.2. --- Basal Medium --- p.37 / Chapter 3.7.3. --- Sucrose --- p.37 / Chapter 3.7.4. --- Auxin Concentration --- p.38 / Chapter 3.7.5. --- Activated Charcoal --- p.38 / Chapter 3.7.6. --- Effect of Ethylene on Rooting --- p.38 / Chapter 3.8. --- Acclimatization --- p.39 / Chapter 4. --- Results / Chapter 4.1. --- Establishment of the Aseptic Culture / Chapter 4.1.1. --- Optimization of the Disinfection Conditions --- p.45 / Chapter 4.1.2. --- Effect of Different Excised Plant Parts on Establishment of the Aseptic Culture --- p.47 / Chapter 4.2. --- Multiplication / Chapter 4.2.1. --- Effect of Basal Medium on Multiplication --- p.57 / Chapter 4.2.2. --- Combination of Plant Hormones --- p.57 / Chapter 4.2.3. --- Effect of Vitamins on Multiplication --- p.58 / Chapter 4.2.4. --- Using Filter Papers as Supporting Materials --- p.59 / Chapter 4.3. --- Rooting Studies / Chapter 4.3.1. --- Effect of Basal Medium Strength --- p.71 / Chapter 4.3.2. --- Effect of Sucrose Concentrations --- p.71 / Chapter 4.3.3. --- Effect of Auxins --- p.71 / Chapter 4.3.4. --- Effect of Activated Charcoal --- p.72 / Chapter 4.3.5. --- Effect of Ethylene --- p.72 / Chapter 4.4. --- Acclimatization / Chapter 4.4.1. --- Effect of Different Kinds of Supporting Materials for Transplanting --- p.90 / Chapter 4.4.2. --- Effect of Different pH on Growth of Explant after Transplanting --- p.90 / Chapter 4.4.3. --- Microcutting --- p.90 / Chapter 5. --- Discussions / Chapter 5.1. --- Factors Involved in Establishment of Aseptic Culture / Chapter 5.1.1. --- Quality of Explant --- p.97 / Chapter 5.1.2. --- The Importance of Sterility --- p.97 / Chapter 5.1.3. --- Prevention of Browning --- p.99 / Chapter 5.2. --- Multiplication of the Tissue / Chapter 5.2.1. --- Nutrient Media Factors --- p.101 / Chapter 5.2.2. --- Growth regulators --- p.101 / Chapter 5.2.3. --- Vitamins --- p.104 / Chapter 5.2.4. --- Supporting Material --- p.106 / Chapter 5.3. --- In Vitro Rooting / Chapter 5.3.1. --- Medium Strength --- p.107 / Chapter 5.3.2. --- Effect of Sucrose --- p.108 / Chapter 5.3.3. --- Effect of Auxins --- p.109 / Chapter 5.3.4. --- Effect of Activated Charcoal --- p.110 / Chapter 5.3.5. --- Ethylene --- p.111 / Chapter 5.4. --- Acclimatization --- p.115 / Chapter 6. --- Summary --- p.117 / Chapter 7. --- References --- p.119