published_or_final_version / abstract / Geography and Geology / Master / Master of Philosophy

Hydraulic conditions (velocity, depth, turbulence) strongly influence the distribution and abundance of organisms in rivers. A diverse hydraulic environment should foster biodiversity, because organisms have different hydraulic preferences. In fact, the relationship between spatial hydraulic diversity and biodiversity is largely presumed, and not well-supported by empirical studies, but it underpins efforts in river restoration and conservation. This is particularly so at the reach scale, indicating a stream- or river-section with large-scale homogeneous geomorphic and hydrological conditions and smaller-scale habitat patches, as perceived by organisms in the community under study. This thesis considers the factors that create spatial hydraulic diversity, and the ways that fish respond. It presents a method to characterise hydraulic diversity, and uses this to describe temporal and spatial changes between reaches. It also demonstrates the use of hydraulic modelling for comparing reaches. Finally, it assesses the Acoustic Doppler Current Profiler (ADCP) as a method to describe hydraulic conditions in a large, open river channel. Swimming ability tests were applied to three small freshwater fish, the pelagic Australian smelt (Retropinna semoni) and common galaxias (Galaxias maculatus ) and the demersal flathead gudgeon (Philypnodon grandiceps). The latter species was the weaker swimmer, but the tests indicated that behaviour also should be considered. A laboratory experiment was designed to investigate how two species with contrasting ecological habits (common galaxias, flathead gudgeon) behave in a diverse hydraulic environment. Habitat choices and activity were monitored in a constructed sinuous channel at three discharges over a 3-hour period. The galaxias favoured the pelagic habitat, and spent 20-60% of the time cruising, whereas the flathead gudgeon preferred the demersal habitat and spent <6% of the time cruising. The flathead gudgeons could access their preferred habitat at all discharges, but the common galaxias were limited by their swimming ability at the highest discharge. Several methods to characterise reaches were compared for eight 3-D model reaches representing the effects of channel form, wood and aquatic plants. The variogram (a measure of the variance between samples as a function of distance) emerged as a superior method because it indicates hydraulic diversity, incorporates the spatial arrangement of hydraulic patches, and facilitates comparisons between reaches. The ADCP proved a quick, reliable means to measure depth and 3-D velocity in rivers. It was effective only in depths >1.5 m, but modified instrumentation may overcome this limitation. Six reaches, including weir-pool and free-flowing sections, were compared at two discharges in the River Murray, Australia. Variograms derived from the ADCP data clearly demonstrated spatial differences between the sections, but temporal differences were less well-defined, suggesting that reaches may retain characteristic hydraulic patterns despite changes in discharge. Opportunities for further research include: the issue of optimal levels of hydraulic diversity for fish and other biota; use of variograms as a tool for field studies of aquatic biota; and measuring reach-scale hydraulic diversity and biodiversity before and after reach manipulation (e.g. the placement of wood), to elucidate the effects of changes in spatial hydraulic diversity on reach biodiversity. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1344602 / Thesis (Ph.D.) - University of Adelaide, School of Earth and Environmental Sciences, 2008

There is growing concern that commonly used Pharmaceuticals and Personal Care Products (PPCPs) and pesticides are entering and contaminating drinking water supplies. The use of targeted quantitation of PPCP has been well established but there is an emerging trend to also screen for and identify unexpected environmental pollutants. Chemicals like pesticides hormones and antibiotics are especially of interest because of proven endocrine disrupting effects and a possible development of bacterial resistance. Powerful screening methods are required to detect and quantify the presence of these compounds in our environment. PPCP encompass a wide range of pollutants, including Endocrine Disrupting Compounds (EDC), pesticides, hormones, antibiotics, drugs of abuse, x-ray contrast agents and drinking water disinfection by-products to name a few. In order to properly assess the effects of these compounds on our environment, it is necessary to accurately monitor their presence. The diversity of chemical properties of these compounds makes method development challenging. LC/MS/MS is able to analyse polar, semi-volatile, and thermally labile compounds covering a wide molecular weight range. The new AB SCIEX TripleTOF™5600 LC/MS/MS was used to profile environmental samples for unexpected pollutants, to identify and characterise the chemical composition and structure of the pollutants, and to quantify (based on intensity) the concentration in collected water samples. Liquid Chromatography coupled to tandem Mass Spectrometry (LCMS/ MS) is able to analyse polar, semi-volatile, and thermally labile compounds covering a wide molecular weight range, such as pesticides, antibiotics, drugs of abuse, x-ray contrast agents, drinking water disinfection by-products etc. More recently there is a growing interest from environmental researchers to also screen for and identify non-targeted compounds in environmental samples, including metabolites and degradates, but also completely unexpected pollutants. The new AB SCIEX TripleTOF™5600 LC/MS/MS system is capable of performing highly sensitive and fast MS scanning experiments to search for unknown molecular ions while also performing selective and characteristic MS/MS scanning for further compound identification and, therefore, is the instrument of choice for this challenging task. General unknown screening workflows do not use a target analyte list and compound detection is not based on any prior knowledge, including retention times and information on possible molecular and fragment ions. Therefore, acquired chromatograms are very rich in information and can easily contain thousands of ions from both any compounds present in the sample as well as from the sample matrix itself. Thus, powerful software tools are needed to explore such data to identify the unexpected compound. Water samples were collected both upstream and downstream of two WWTPs (Seymour and Fort Beaufort) and were directly injected on the AB SCIEX TripleTOF™5600 LC/MS/MS after being filtered. 15 sample points along the Kat River, ranging from a point as close to the source as possible to a point just before it joins the Great Fish River were used. The samples collected from the source were used as the control in each of the experiments, the assumption being the closer you get to the source, the less contaminated the water would be for the analysis of pesticides. Points were selected where the Kat River crosses the R67 or on farms where the river was accessible using farm roads. Samples were collected from October 2013 to November 2014.The Peak view software and Analyst software were used in the analysis of PPCPs. The XIC Manager allows you to manage large lists of compounds and perform automatic extracted ion chromatogram (XIC) calculations and review results operations. The results were displayed in the chromatogram pane and the XIC table (see results). The results reported here in this thesis indicate that there is contamination in the Kat River water due to both pesticides and PPCPs. The results also indicate that the food products are also contaminated and hence both the Kat River agricultural produce and its water need to be closely monitored for both pesticide and PPCPs contaminants. Further studies to investigate the quantitative levels of pesticides and PPCPs in the Kat river water to determine if the concentration levels of the detected pesticides are below the reported Maximum Residues Limits will be explored in the future.

published_or_final_version / Ecology and Biodiversity / Doctoral / Doctor of Philosophy

This thesis examined the impact of anthropogenic alterations in four riverine catchments of the Eastern Mt. Lofty Ranges, South Australia, to identify if creek restoration via environmental flows is a viable management option and if so, to determine; 1) Whether an aquatic/riparian viable seed bank was present and if so what was its composition 2) The ecological condition of selected riverine reaches. This in combination with the seed bank study would identify those creeks that would most benefit from the imposition of environmental flows 3) The response of key species to the water regimes likely to result from the imposition of environmental flows 4) The influence of nutrient enrichment under a fluctuating water level and to use this information to formulate best practice policy for environmental flows 5) Whether aquatic plants promoted by environmental flows were a significant fraction of the diet for higher trophic levels represented by Trichopterans and Amphipods. The seed banks were of comparable density (ranging from 4,000 to 110,000 seeds m-2) and species richness (ranging from 13 to 20 aquatic / riparian species) to the seed banks of other Australian rivers and wetlands, but this varied significantly among riverine sections and across catchments. Out of a total of 81 species recorded, 51 were classified as terrestrial (63% of all species recorded). What is of greater concern was the number of exotic (both aquatic and terrestrial) species recorded: 43% of the species recorded in the Angas, 47% of the species in the Finniss, 39% of the species in Tookayerta creek and 43% of the species recorded in Currency creek were exotic, which are significantly higher in comparison to other Australian studies. There were 24 to 28 aquatic/riparian species recorded in the extant vegetation of each catchment that were not recorded in their seed banks. Likewise, a number of species (3 to 7) were recorded in each catchments seed bank that were not recorded in the extant vegetation of those catchments. A species of particular interest is Crassula sieberana, which is on the State endangered plant species list. Indices for assessing the ecological condition, health or integrity of a river or riparian habitat were employed to investigate the relationship between the river/riparian habitat and the land and water management practices associated with those habitats. Of the four catchments surveyed, each catchment identified a unique set of site parameters (subindex indicators) that were strongly correlated with its ecological condition. Indicator species analyses revealed pasture grasses to be a significant indicator of reaches in very poor condition (p = 0.0010) along the Finniss and Baumea juncea of those reaches in good condition (p = 0.0230). Along the Angas, Cotula coronopifolia was an indicator of those reaches in average condition (p = 0.0240) and along Currency creek, Cladium procerum was an indicator of those reaches in good condition (p = 0.0190). However, when all 115 surveyed reaches were analysed together, those reaches of average to excellent ecological condition were all strongly correlated (R2 = 0.50) with the subindex indicators: bank stability, % riparian cover, grazing, fenced, aquatic wood, and width of the riparian vegetation. This would indicate that these subindex indicators are the main site parameters determining the ecological condition of a riverine reach and hence its restoration potential. Those catchments or sub-catchments containing a high proportion of reaches classified to be in poor to very poor condition had significantly reduced seed banks. The influence of water level fluctuations (±15 cm, ±30 cm and ±45 cm) on the growth of four species of emergent macrophytes (Cyperus vaginatus, Phragmites australis, Typha domingensis and Triglochin procerum) were species dependent. These species naturally inhabits different zones across the elevation gradient. C. vaginatus, which has a high elevation preference, was strongly inhibited by increasing water depth and fluctuations in water levels. In contrast, species with an intermediate elevation preference, such as Phragmites australis and Typha domingensis, were more tolerant to both depth and water level fluctuations. However, the biomass and relative growth rate (RGR) of T. domingensis and P. australis were depressed when grown under the combination of deep elevation and a highly fluctuating water level (±45 cm). Between the static and ±45 cm amplitude treatments, growth of T. domingensis was inhibited by 52%. The growth of P. australis appeared to be enhanced by fluctuating water levels and only showed a severe drop-off in growth in the deep elevation, ±45 cm amplitude treatment. In C. vaginatus the RGR was dependent of the average emergent surface area (and the implied rate of carbon acquisition)(p<0.0001; r2=0.7196; F=87.276; n=36; RGR (mg g-1 day-1) = -5.096 + 4.313 × ln (Average emergent surface area (cm2)), but this was not the case in P. australis and T. domingensis (p>0.05) even when the photosynthetic canopy was partially inundated by rising water levels. Yet these two species demonstrated different growth rates when grown under different water regime amplitudes and at different elevations. Growth of T. procerum did not respond to either amplitude or elevation, but its RGR remained negative. This suggests that another factor(s) was limiting the growth of P. australis, T. domingensis and possibly T. procerum, a factor that varies with water level. Cyperus gymnocaulos had significantly increased plant performance (p <.0001) with increased nutrient loading rates but this effect was significantly reduced under a fluctuating water regime (p =0.0007). Remarkably, under a fluctuating regime, P. australis had a significant reduction in performance with increased nutrient loading rates (p =0.0013), whereas T. domingensis performance was significantly limited (p =0.034) even with increased nutrient loading rates. T. procerum too had increased plant performance with increased nutrient loading rates but this effect was reduced under a fluctuating regime. The morphological response by T. procerum demonstrates that it is mainly limited by the nutrient loading rates and not the water regime. However, it was significantly limited/reduced by its increased turnover rates caused by a stochastic fluctuating water regime. Illustrating that in fact the effects of nutrient enrichment on T. procerum were independent of water regime but bearing in mind that water regime is the primary factor determining the productivity of this species. For those species with higher elevation preferences, e.g. C. gymnocaulos, or low elevation preference, e.g. T. procerum, the effects of nutrient loading are independent of water regime, whereas those species with an intermediate elevation preference, e.g. P. australis and T. domingensis the effects of nutrient loading are largely dependent on the water regime. Amphipoda and Trichoptera selectively fed on succulent semi-emergent macrophytes across sites of average to excellent ecological condition (31-64% to 65-97% of diet), depending on availability. These semi-emergent macrophytes contained the lowest C:N ratio (≈10:1), closest to that of their consumers (≈5:1) and therefore the highest nutritional content. In degraded riverine reaches, there were limited food resources available, hence course particulate organic matter (CPOM) formed the main dietary components of Amphipoda (20-53% of diet) even though it had the highest C:N ratio (≈40:1). At site VP. 1, filamentous algae was the main dietary component of Trichoptera (48-64% of diet) due to its availability and its low C:N ratio (≈14:1) in comparison to the other primary sources available. The imbalanced consumer-resource nutrient ratios in these degraded riverine reaches are likely to impose constraints on the growth and reproduction of their aquatic shredder communities with probable knock-on effects at higher trophic levels. The installation of environmental flows to restore and promote aquatic/riparian plant communities, which in turn would benefit higher trophic organisms, is a viable and realistic management option along selected reaches. Those selected reaches contain a significant aquatic/riparian seed bank and with sufficient physical habitat remaining to promote their germination and establishment. However, the imposition of environmental flows as a control measure to prevent the colonisation and dominance of particular species (T. domingensis and P. australis) was deemed to be redundant as a management technique given the limited water resources available. / Thesis (Ph.D.) -- School of Earth and Environmental Sciences, 2007

Water-related disease is a problem faced by many communities in the Mngeru and Msunduzi River valleys, both those living in a rural setting, and those within thedty bounds. Treatment and management of water-related diseases such as SchistosorrUasis and Hepatitis are, however, overshadowed by the priority management of more serious illnesses such as HIV/ AIDS, and are therefore at risk of spreading unchecked. These diseases, while not often fatal, have a high morbidity and place unnecessary burden on communities and individuals that could otherwise make a positive contribution to the social and economic structure of the country. The impacts of water-related disease are felt by all water-users, including those recreational users such as fishermen, swimmers and canoeists. Following a discussion of the issues and impacts of water-related disease at both a global and local scale, the research formulated a survey of the impact of water-related disease on canoeists training and racing on the Mngeni and Msunduzi Rivers as a starting point in determining the impacts on all communities using the resource. The methods employed were twofold, a Schistosoma haematobium infection- survey was conducted using urine samples; and a questionnaire-type survey of participants in the 2006 Dusi Canoe Marathon was conducted. The results of the Schistosomiasis survey revealed that 4.07% (20/491) of the respondents were positive in this survey. Analysis of the questionnaire filled in by all participants further revealed that 73% of respondents tested positive for Schistosomiasis infection at some point in their canoeing career. The post Dusi Marathon questionnaire survey was answered by 941 (54 %) participants. A total of 588 (63 %) reported experiencing illness as a result of taking part in the 2006 event. A further 362 individuals reported having experienced water-related illness on previous Dusi Marathons, indicating that 77% of the respondents have been ill on the Dusi in this and previous years' events. In addition, water quality samples were taken during the race and were shown to be significantly higher than the guidelines for safe recreational use of water resources. The results also showed a radical overnight change in the E.coli levels following a heavy rain event on the evening before the start of the race. The research revealed that there is a serious problem of water contamination and resultant spread of disease among canoeists using the rivers for recreational and professional sport. Contamination resulting from failure of sewage processing facilities during high rainfall events and industrial discharge into the river system results in excessive and unacceptable levels of E.coli and other water-related pathogens that are a severe health risk not only to canoeists but to all communities utilising this water resource. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2006.

The nutrient dynamics in two contrasting estuaries and in the adjacent nearshore environment along the south-east coast of South Africa was investigated seasonally. Due to an inter-basin transfer of water from the Gariep Dam to the Great Fish River, the Great Fish estuary is a fresh water dominated, terrestrially driven system with an annual fresh water inflow of 250 – 650 x 10⁶ m³ per year. In contrast, the Kariega estuary is a fresh water deprived, marine dominated system with a fresh water inflow estimated at 2.5 – 35 x 10⁶ m³per year. The reduced fresh water inflow into the estuary is attributed to regular impoundments along the Kariega River. Water samples were collected from surface and subsurface layers along the length of the estuaries as well as from a series of transects occupied in the nearshore environment. Samples were analysed for nitrate, nitrite, ammonium, phosphate and silicate. Temperature and salinity were recorded at each station. A Land-Ocean Interactions in the Coastal Zone (LOICZ) budget was constructed for each estuary to describe the role of ecosystem-level metabolism as either a sink or a source of phosphorus, nitrogen and carbon. Seasonal variation in physico-chemical properties and nutrient concentrations in the Kariega estuary was minimal due to constant low inflow, while in the Great Fish estuary, concentrations varied in response to changes in flow rate. Nutrient concentrations were consistently higher in the Great Fish estuary than in the Kariega estuary, largely reflecting differences in fresh water inflow. During periods of high flow (32.92 m³.s⁻¹in the Great Fish River) dissolved inorganic nitrogen (DIN) concentrations in the Great Fish estuary were an order of magnitude higher than those recorded in the Kariega estuary. Results of the LOICZ budgeting procedures revealed that in spite of the contrasting hydrodynamic features, the estuaries behave in largely the same manner – both predominantly sources of nutrients with heterotrophic processes dominating over autotrophic actions and both were net denitrifyers during all surveys. This was, however, due to different sets of processes operating in the two estuaries, namely low nutrient concentrations resulting in microbial activity in the Kariega estuary, and riverine influx of nutrients and phytoplankton combined with a short residence time of the water in the Great Fish estuary. In the marine nearshore environment, higher nutrient concentrations were recorded adjacent to the Great Fish estuary than offshore of the Kariega estuary. This was due to a surface plume of less saline water leaving the Great Fish estuary, which acted as an ‘outweller’ of nutrients. Offshore of the Kariega estuary, on the other hand, the nutrient concentrations were characteristic of marine waters due to a lack of fresh water outflow from the estuary. Nutrient concentrations in the marine environment adjacent to the Kariega estuary were, at times, higher than those recorded within the estuary. This observation supports previous statements which suggest that the Kariega estuary is not an ‘outweller’ of dissolved nutrients and particulate material, but rather an extension of the marine environment.

Thesis (MSc)--University of Stellenbosch, 2003. / ENGLISH ABSTRACT: The River Health Programme (RHP) is an assessment tool for monitoring the ecological state of rivers to ensure that they remain fit for use by present and future generations. This study, forming part of a RHP assessment conducted on the south-western Cape Hout Bay, Lourens and Palmiet Rivers, has the aim to (1) zone the rivers for representative site selection, (2) assess their habitat integrity (HI), (3) determine the influence of land use on riverine HI and (4) assess the river water quality at the time of the RHP assessments. (1) The desktop geomorphological zonation method used in RHP assessments has not been sufficiently previously tested on short rivers draining the Western Cape Mountains. The Lowland River Zone of the rivers studied, as well as the Hout Bay River’s Upper Foothill Zone, were found to have steeper gradients than expected, probably due to these rivers being shorter and consequently steeper than any on which the method was previously tested. The notion of one gradient river classification system being applicable throughout South Africa, with its diverse geology and climate, is unlikely. Rather a classification system modified for various physiographic features regions or by a factor based on river length is more realistic. (2) Although there is a general longitudinal decrease in HI downstream along the Hout Bay and Lourens Rivers, coinciding with increased anthropogenic activities, HI improves in the Palmiet River’s lower reaches through the Kogelberg Nature Reserve. Surrounding land use thus seems to be a major determinant of HI. Although the Index of Habitat Integrity (IHI) used appears to achieve its aim, it was found to be subjective. Categorisation of the IHI scoring is suggested. (3) The amount of natural versus disturbed land use occurring upstream of a site at a regional and local scale, is a good predictor of riverine HI. Regional alien forestry and local urbanisation have significantly strong negative effects on instream (r2 = -0.80, r2 = 0.80, p<0.05) and riparian (r2 = -0.81, r2 = -0.83, p<0.05) HI. Different land use types therefore appear to affect riverine HI at differing scales and thus managers must not only think on a local but also a catchment scale. (4) In the Hout Bay River, a filtering system (e.g. wetland) appears to improve the water quality between the middle and lower reaches. Along the Lourens River, high total dissolved salts, conductivity and inorganic nitrogen concentrations in the middle reaches are cause for concern. Along the Palmiet River there appeared to be insufficient oxygen to support most aquatic life forms at Grabouw. Impoundments in the middle reaches act as sinks for nutrients and salts, but the Huis and Krom tributaries downstream then appear to degrade the water quality of the Palmiet River’s lower reaches within the Kogelberg Nature Reserve. Together with the results of simultaneous biotic assessments, these results should be used to develop management actions to improve the ecological health of these rivers. The results have been used in a State-of-Rivers Report for the south-western Cape. / AFRIKAANSE OPSOMMING: Die Riviergesondheidsprogram (RGP) is 'n asseseringsinstrument wat die ekologiese stand van riviere monitor om te verseker dat hulle steeds bruikbaar bly vir huidige en toekomstige geslagte. Hierdie studie maak deel uit van 'n RGP-assessering van die Lourens-, Houtbaai- en Palmietrivier in die Suidwes-Kaap en het ten doel om (1) die riviere te soneer vir verteenwoordigende terreinseleksie, (2) die habitat-integriteit (HI) te assesseer, (3) die invloed van grondgebruik op rivier-HI te bepaal en (4) die kwaliteit van rivierwater tydens die RGP-assesserings te bepaal. (1) Die geomorfologiese-soneringsmetode wat in RGP-assesserings gebruik word, is nog nie voorheen genoegsaam vir die kort riviere wat die Wes-Kaapse berge dreineer, getoets nie. Daar is bevind dat die studiegebied riviere in die laagland-sones skerper gradiënte het as verwag, gehad het. Dit kan moontlik toegeskryf word aan die riviere wat korter en dus steiler is as enige van dié wat voorheen met die metode getoets is. Die moontlikheid dat een gradiëntklassifikasiestelsel vir riviere regdeur Suid-Afrika met sy diverse geologie en klimaat toegepas kan word, is onwaarskynlik. 'n Klassifikasiestelsel aangepas vir verskillende fisiografiese streke of met 'n faktor gebaseer op rivierlengte, is meer realisties. (2) Alhoewel HI stroomaf langs die Lourens- en Houtbaairivier in die algemeen longitudinaal saam met die toename in antropogeniese aktiwiteite afneem, verbeter die Palmietrivier se HI waar dit laer af deur die Kogelbergnatuurreservaat vloei. Die gebruike van aanliggende grond blyk dus 'n belangrike bepaler van HI te wees. Die Indeks van Habitatintegriteit (IHI) bereik klaarblyklik die vereiste doel, maar is te subjektief. Kategorisering van die IHI-waardes word voorgestel. (3) 'n Goeie voorspeller van rivier-HI is die hoeveelheid natuurlike teenoor versteurde grondgebruik stroomop van 'n terrein op 'n streeks- en lokale skaal. Die sterk negatiewe effek van uitheemse plantegroei in die omgewing en lokale verstedeliking op stroom- (r² = -0.80, r² = 0.80, p<0.05 ) en oewer-HI (r² = -0.81, r² = -0.83, p<0.05) is beduidend. Verskille in tipe grondgebruik beïnvloed rivier-HI op verskillende vlakke; bestuurders moet dus plaaslik en aan die opvanggebied dink. (4) In die Houtbaairivier lyk dit asof 'n filtreringstelsel (bv. vleigrond) die waterkwaliteit tussen die middel- en lae gedeeltes verbeter. In die loop van die Lourensrivier is hoë totale opgeloste soute, geleidingsvermoë en anorganiese stikstofkonsentrasies in die middelgedeelte 'n rede tot kommer. In die Palmietrivier by Grabouw was die suurstof te min om die meeste akwatiese lewensvorme te onderhou. Opgedamde water in die middel gedeeltes dien as 'n sink vir voedingstowwe en soute, maar dit lyk asof die Huis- en Kromrivier die waterkwaliteit van die Palmietrivier stroomaf in die Kogelbergnatuurreservaat degradeer. Saam met die resultate van gelyktydige biotiese assesserings, kan hierdie resultate gebruik word vir die ontwikkeling van bestuursaksies om die ekologiese toestand van hierdie riviere te verbeter. Die resultate is gebruik in 'n toestand-van-riviere-verslag vir die Suidwes-Kaap.

The Green Drop Certification Programme, launched in 2008 alongside the Blue Drop Certification Programme, aims to provide the Department of Water Affairs with a national overview of how municipalities and their individual wastewater treatment works (WWTW) are complying with licence conditions set by the National Water Act (NWA) (No. 36 of 1998; DWAF 1998) and the Water Services Act (No. 108 of 1997; DWAF 1998). By publishing the results of each municipality’s performance, the programme aims to ensure continuous improvement in the wastewater treatment sector through public pressure. The programme has been identified by this project as a necessary linking tool between the NWA and the Water Services Act to ensure protection and sustainable use of South Africa’s natural water resources. It does this through assisting municipalities to improve their wastewater treatment operations which in theory will lead to discharged effluent that is compliant with discharge licence conditions. These discharge licences form part of the NWA’s enforcement tool of Source Directed Controls (SDC) which help a water resource meet the ecological goals set for it as part of Resource Directed Measures (RDM). The link between meeting the required SDC and achieving the RDM goals has never been empirically tested. This project aimed to determine the present ecological condition of the Uie River, a tributary of the Sundays River which the Sundays River Valley Municipality (SRVM) discharges its domestic effluent into. It then determined whether the SRVM’s WWTW was complying with the General Standard licence conditions and what the impact of the effluent on the river was through the analysis of monthly biomonitoring, water chemistry and habitat data. Lastly, the project examined the effectiveness of the Green Drop Certification Programme in bringing about change in the SRVM’s wastewater treatment sector, which previously achieved a Green Drop score of 5.6 percent. It wanted to examine the underlying assumption that a WWTW which improves its Green Drop score will be discharging a better quality effluent that will help a water resource meets the RDM goals set for it. The Kirkwood WWTW did not have a discharge licence at the time of assessment and was thus assessed under the General Standard licence conditions. It was found that the Kirkwood WWTW was not complying with the General Standard discharge licence conditions in the Uie River. This was having a negative impact on the river health, mainly through high concentrations of Total Inorganic Nitrogen (TIN-N), orthophosphate and turbidity. The SRVM should see an improvement in its Green Drop score for the Kirkwood WWTW. However, the municipality showed no implementation of necessary programmes. Implementation of these programmes would help the SRVM meet the General Standard licence conditions (part of SDC) which would help the Uie River meet the RDM goals set for it.