Thesis (PhDEng)--University of Stellenbosch, 2002. / ENGLISH ABSTRACT: The interaction between moving water and the physical attributes of a river, as displayed by the channel morphology, determines the availability of physical habitat for aquatic species and thus also the condition of the ecosystem. As such, the environmental flow assessment process requires knowledge on how changes in the flow regime will affect both the morphological and hydraulic conditions within a river channel. With the increasing development of water-related infrastructure in mountain regions, knowledge of the morphological and hydraulic characteristics of rivers in the upper catchment areas is very important. Cobble and boulder bed rivers in the Western Cape are typical examples. They are characterized by steep gradients, great variability in sediment size and relatively low flow depths. The bed configuration contains a series of pools, steps, rapids, riffles and plane beds, while energy losses are high as a result of turbulence and hydraulic jumps. Due to their wideranging morphological and associated hydraulic attributes, the physical habitats within these rivers are extremely diverse, both on a spatial and temporal scale. This study addresses the interaction between moving water and the physical attributes of cobble and boulder bed rivers. Empirical, semi-empirical and theoretically based models are developed which define the hydraulic and morphological related characteristics of environmental flow components in cobble and boulder bed rivers. They cover macro scale channel deformation, the scouring of sand from the interstitial spaces between the cobbles as well as velocity-depth relationships, which prove to be key components in the assessment of environmental flow requirements in cobble and boulder bed rivers. / AFRIKAANSE OPSOMMING: Die wisselwerking tussen die vloeiende water en die fisiese eienskappe van 'n rivier, soos beskryf deur die morfologie, bepaal die beskikbaarheid van habitat vir akwatiese spesies en gevolglik ook die welstand van die ekosisteem. Kennis omtrent die impak van 'n veranderde vloei regime op beide die morfologiese en die hidrouliese toestande in 'n rivierloop is dus nodig vir die bepaling van omgewingsvloeibehoeftes. Met die toenemende ontwikkeling van water-verwante infrastruktuur in bergagtige gebiede is 'n grondige kennis van die morfologiese en hidrouliese eienskappe van riviere in hierdie bo-opvanggebiede gebiededend noodsaaklik. Spoelklip riviere in die Wes-Kaap is tipiese voorbeelde van sulke riviere. Hierdie riviere word gekenmerk deur steil hellings, 'n wye verskeidenheid sedimentgroottes, relatiewe lae vloeidieptes en hoë energieverliese as gevolg van turbulensie. Verder bevat die ri vierbed afwisselend poele en stroomversnellings en gevolglik word 'n wye verskeidenheid habitat tipes in hierdie riviere aangetref. Hierdie studie fokus op die interaksie tussen bewegende water en die fisiese eienskappe van spoelklip riviere. Met behulp van empiriese, semi-empiriese en teoretiese modelle word die morfologiese en hidrouliese eienskappe van omgewingsvloeibehoeftes in spoelklip riviere aangespreek. Dit sluit makroskaal kanaal vervorming, die uitskuur van sand tusen die spoelklippe asook die verwantskap tussen vloeisnelheid en vloeidiepte in. Hierdie aspekte kan beskou word as van die sleutel elemente vir die bepaling van omgewingsvloeibehoeftes in spoelklip riviere.

Thesis (MTech (Biomedical Technology))--Cape Peninsula University of Technology, 2012. / In many developed countries worldwide the provision of safe, clean water is an expected commodity. In South Africa however, as in most developing countries, the access and supply of water safe for human consumption is challenged or complicated by pollution and more recently water availability. Point-source pollutants in surface- and groundwater are normally the most concentrated closest to the pollutant source (such as the end of a pipe or an underground injection system). Examples of point-source pollution are commercial and industrial businesses, that often discharge waste such as solvents and heavy metals from their operations. In contrast, non-point-source pollution occurs due to runoff moving across or through the ground and absorbing and accumulating pollutants which eventually end up in streams, rivers and dams. The lack of waste removal and adequate sanitation facilities results in the disposal of faecal matter and sewage into storm water drains which flow directly into the river systems contributing to the incidence of diseases such as gastroenteritis, diarrhoea and chronic lung ailments, caused by waterborne pathogenic bacteria, viruses and fungi.

Thesis (MSc)--University of Stellenbosch, 2002. / ENGLISH ABSTRACT: In this study, the Fish Assemblage Integrity Index (FAll) was applied on three rivers within the southwestern Cape. This index uses fish as indicators of biological aquatic integrity and is based on indigenous species expected to be present in biological fish habitats. Fish integrity classes were calculated for each of the sites in the three rivers studied. Sites 2 and 4 within the Lourens River were rated as Class C, whereas sites 1 and 3 were rated as Class F and Class D respectively. Sites 1, 2 and 4 within the Palmiet River were rated Class F, whereas sites 3 and 5 were rated as Class E and Class D respectively. Site 1 within the Hout Bay River was rated as a Class F site, in addition to sites 2 and 3 been rated as Class A. It is however, suggested that the FAll needs to be adjusted to accommodate the general low species richness experienced in the southwestern Cape. In addition to the FAll been applied, the effects of long-term exposure to subtle water quality changes associated with human activities, specifically potential estrogenic compounds in fish from the Lourens River were also investigated. The production of the yolk precursor lipoprotein complex, vitellogenin (Vtg) produced in the liver under estrogen control was employed as biomarker for environmental estrogen exposure. Male fish from the Lourens River were studied using SDS-PAGE gel electrophoresis. Results indicated that 60% of male fish showed the presence of Vtg in their plasma. Abnormal gonad morphology in male and female fish were also assessed using standard histological procedures. Results from this study indicated no observed abnormalities in either male or female gonads. The immediate presence of endocrine disrupters with estrogen activity was investigated by screening water samples from the Lourens, Palmiet and Hout Bay Rivers for estrogen activity. Results indicated that none of the samples appeared to be cytotoxic. In addition, estrogen activity of water samples was also investigated by in vitro culturing of water samples with frog, Xenopus laevis, liver slices. Results indicated that none of the water samples from the three rivers studied indicated estrogenic activity. Although cytotoxicity and estrogen activity results were negative, the production of Vtg in male fish suggests further research regarding the presence of estrogenic substances in these rivers. / AFRIKAANSE OPSOMMING: In die huidige studie is die 'Fish Assemblage Integrity Index' (FAIl) toegepas op drie rivere in die suidwes Kaap. Hierdie indeks gebruik visse as bioindikatore van biologies akwatiese integriteit en is gebasseer op die inheemse visspesies wat verwag word in biologiese vishabitatte. Integriteitsklasse is bepaal vir elke studieterrein in die drie rivere wat ondersoek is. 'n Klas C is bepaal vir studieterreine 2 en 4 in die Lourensrivier. Klas F en Klas D is bepaal vir studieterreine 1 en 3 in die rivier onderskeidelik. 'n Klas F is bepaal vir studieterreine 1, 2 en 4 en Klas E en Klas D bepaal vir studieterreine 3 en 5 in die Palmietrivier onderskeidelik. 'n Klas F is bepaal vir studieterrein 1 in die Houtbaairivier waar 'n Klas A bepaal is vir studieterreine 2 en 3. Dit word egter voorgestel dat die FAII aangepas moet word om die algemene lae spesierykheid wat ervaar word in die suidwes Kaap te akkomodeer. Die reaksie van visse, afkomstig van die Lourensrivier, op die langtermyn blootstelling aan estrogeniese stowwe is ook bestudeer. Spesifieke reaksies van endokriene versteuring, soos vitellogeen (Vtg) produksie in manlike visse is ondersoek deur middel van SDS-PAGE gel elektroforese. Resultate toon dat in 60% van die manlike visse Vtg in die plasma teenwoordig was. 'n Ondersoek na abnormale gonade morfologie in manlike en vroulike visse van die Lourensrivier is deur standard histologiese prosedures gedoen. Resultate hiervan dui op geen sigbare abnormaliteite in die gonades nie. Die onmiddelike teenwoordigheid van endokriene versteurders is bestudeer deur die sitotoksisiteit van watermonsters afkomstig van die Lourens, Palmiet en Houtbaai riviere te bepaal. Resultate dui aan dat geen monsters sitotoksies was nie. Die estrogeenaktiwiteit van die watermonsters is ook ondersoek deur van in vitro kulture van watermonsters saam met padda, Xenopus leavis, lewersnitte gebruik te maak. Geen estrogeniese aktiwiteit is in die watermonsters gevind nie. Al is die sitotoksisiteit en estrogeenaktiwiteit resultate negatief, dui die produksie van Vtg in manlike visse op die noodsaaklikheid van verdere navorsing ten opsigte van die teenwoordigheid van estrogeniese stowwe in drie riviere.