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Phenomics, Genomics and Genetics in Plasmodium vinckeiRamaprasad, Abhinay 11 1900 (has links)
Rodent malaria parasites (RMPs) serve as tractable models for experimental genetics,
and as valuable tools to study malaria parasite biology and host-parasitevector
interactions. Plasmodium vinckei, one of four RMPs adapted to laboratory
mice, is the most geographically widespread species and displays considerable phenotypic
and genotypic diversity amongst its subspecies and strains. The phenotypes
and genotypes of P. vinckei isolates have been relatively less characterized compared
to other RMPs, hampering its use as an experimental model for malaria. Here, we
have studied the phenotypes and sequenced the genomes and transcriptomes of
ten P. vinckei isolates including representatives of all five subspecies, all of which
were collected from wild thicket rats (Thamnomys rutilans) in sub-Saharan Central
Africa between the late 1940s and mid 1960s. We have generated a comprehensive
resource for P. vinckei comprising of five high-quality reference genomes, growth
profiles and genotypes of P. vinckei isolates, and expression profiles of genes across
the intra-erythrocytic developmental stages of the parasite. We observe significant
phenotypic and genotypic diversity among P. vinckei isolates, making them particularly
suitable for classical genetics and genomics-driven studies on malaria parasite
biology. As part of a proof of concept study, we have shown that experimental genetic
crosses can be performed between P. vinckei parasites to potentially identify
genotype-phenotype relationships. We have also shown that they are amenable to
genetic manipulation in the laboratory.
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Células progenitoras CD34+ durante a ampliação esplênica na malária experimental de roedores. / CD34+ progenitor cells during spleen amplification in experimental rodent malaria.Hermida, Felipe Pessoa de Melo 24 September 2007 (has links)
A malária é uma infecção causada por plasmódios, cujo controle depende do baço, o responsável pelo clareamento dos eritrócitos parasitos. O aumento da parasitemia induz uma ampliação do baço para resolver a infecção, onde participam células precursoras que apresentam CCD34+ na sua superfície. Estudamos a distribuição e a quantidade de células CD34+ em baços de roedores durante malárias de roedores, para compreender sua participação na ampliação do baço e no controle da infecção. Camundongos C57Bl/6j infectados com as cepas AJ e CR de Plasmodium chabaudi, e com a cepa ANKA de Plasmodium berghei, tiveram seus baços removidos e encaminhados para histologia e citometria de fluxo. A distribuição das células CD34+ mostrou-se mais intensa no 4º dia p.i. e menos intensa no 8º dia p.i.. As células CD34+ livres, por citometria de fluxo, surgem com uma onda no 4º dia p.i.. Sua quantidade é similar entre os modelos de P. chabaudi, mas diferente no P. berghei. Neste trabalho, o influxo de células CD34+ no baço não se relaciona com o controle da infecção. / Malaria is caused by Plasmodium sp., which control depends on the spleen, responsible for parasite clearing. The increase of parasitemia implies in spleen amplification to control the infection, with participation of CD34+ cells. We studied the distribution and amount of CD34+ cells in spleen during rodent malaria, to define the role of those cells in spleen amplification and infection control. C57Bl/6j mice were infected with strains CR and AJ of Plasmodium chabaudi, and ANKA strain of Plasmodium berghei. The spleen was removed and processed for histology and flow cytometry. Spleen CD34+ cells was increased in 4th day, p.i., and decreases in 8th day p.i. in all models. By flow cytometry, free CD34+ cells appears as a wave in the 4th day p.i.. P. chabaudi models presented the same level of those cells, which was larger in the P. berghei mice. In this work, increase of spleen CD34+ cells do not correlate with infection control.
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Células progenitoras CD34+ durante a ampliação esplênica na malária experimental de roedores. / CD34+ progenitor cells during spleen amplification in experimental rodent malaria.Felipe Pessoa de Melo Hermida 24 September 2007 (has links)
A malária é uma infecção causada por plasmódios, cujo controle depende do baço, o responsável pelo clareamento dos eritrócitos parasitos. O aumento da parasitemia induz uma ampliação do baço para resolver a infecção, onde participam células precursoras que apresentam CCD34+ na sua superfície. Estudamos a distribuição e a quantidade de células CD34+ em baços de roedores durante malárias de roedores, para compreender sua participação na ampliação do baço e no controle da infecção. Camundongos C57Bl/6j infectados com as cepas AJ e CR de Plasmodium chabaudi, e com a cepa ANKA de Plasmodium berghei, tiveram seus baços removidos e encaminhados para histologia e citometria de fluxo. A distribuição das células CD34+ mostrou-se mais intensa no 4º dia p.i. e menos intensa no 8º dia p.i.. As células CD34+ livres, por citometria de fluxo, surgem com uma onda no 4º dia p.i.. Sua quantidade é similar entre os modelos de P. chabaudi, mas diferente no P. berghei. Neste trabalho, o influxo de células CD34+ no baço não se relaciona com o controle da infecção. / Malaria is caused by Plasmodium sp., which control depends on the spleen, responsible for parasite clearing. The increase of parasitemia implies in spleen amplification to control the infection, with participation of CD34+ cells. We studied the distribution and amount of CD34+ cells in spleen during rodent malaria, to define the role of those cells in spleen amplification and infection control. C57Bl/6j mice were infected with strains CR and AJ of Plasmodium chabaudi, and ANKA strain of Plasmodium berghei. The spleen was removed and processed for histology and flow cytometry. Spleen CD34+ cells was increased in 4th day, p.i., and decreases in 8th day p.i. in all models. By flow cytometry, free CD34+ cells appears as a wave in the 4th day p.i.. P. chabaudi models presented the same level of those cells, which was larger in the P. berghei mice. In this work, increase of spleen CD34+ cells do not correlate with infection control.
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