Many groups of protists, also known as microbial eukaryotes, remain understudied. One such group, the superfamily Sainouroidea in the supergroup Rhizaria has been shown to be a molecularly diverse and divergent clade by recent environmental sequencing. Here we isolated and analyzed cultures of Sainouroid amoebae using 18S rDNA sequencing, light microscopy, and TEM. Using a molecular species concept we described four novel genera and twelve novel species in Sainouroidea. Another understudied group Cutosea, in the supergroup Amoebozoa, contains the ATCC isolate PRA-29, which is an amoeba that has been used in many phylogenomic datasets. PRA-29 was incorrectly identified as Pessonella sp. Here we studied PRA-29 in detail using light microscopy, TEM, and SEM and re-classify it as Armaparvus languidus novel genus, novel species. We show that a cell coat of microscales separated from the cell membrane is a unique trait found in all known Cutosean amoebae.