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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
71

Grazing and Feeding Management of Lactating Dairy Cows

Soriano, Felix Diego 12 August 1998 (has links)
Two studies were conducted during the grazing season of 1997. Study 1 consisted of three Experiments, and the objectives were to compare milk production and composition, body weight change and body condition score, and to determine time patterns of grazing between cows supplemented with different forms and amounts of corn. Also rumen fermentation parameters were measured in cows supplemented with two different types of corn. In study 2, milk yield was measured when grazing pasture was supplemented to lactating Holstein cows fed a typical TMR diet. Predominantly orchardgrass pastures with lesser amounts of white clover and Kentucky bluegrass were grazed during both studies. In Experiment 1, 36 Holstein cows were supplemented either with 6, 6, 6, or 4 kg/d DM of high moisture corn, coarsely ground corn, finely ground corn, or high moisture corn in two equal feedings, respectively. Milk yield was similar (30.3 kg/d) among treatments. Milk protein (2.97%) and MUN (14.7 mg/dl) did not differ among treatments. Body weight change and body condition score change were similar among treatments (23.1 kg and -0.24). During Experiment 2, four rumen-cannulated cows in mid-lactation were supplemented 6 kg/d DM of either coarsely ground corn or high moisture corn in two equal feedings. After the p.m. milking, ruminal pH was measured and rumen fluid samples were collected to determine ammonia N and VFA. While grazing, this was repeated at 0.5, 1, 2, 3,...8 h post-corn feeding (0 h). Ruminal pH was similar for both corn supplements and was lowest (5.9 and 5.8) at 5 and 8 h, respectively. Rumen ammonia N concentrations started to increase approximately 2 h after cows began grazing, reaching maximum levels 5 h later. In Experiment 3, the number of cows grazing, lying, or standing were recorded every half hour, for two consecutive days, while grazing. Cows grazed an average of 6.4 h/d, 4.1 h in the afternoon and 2.3 h in the morning. Similarity in milk production, milk composition, BW change, and BCS between treatments indicates that the quality and availability of pasture permitted equal response regardless of the type or amount of corn supplemented. Fifty four Holstein cows in mid lactation were used in Study 2. Cows were fed either a TMR diet only, or were fed TMR during half of the day (after the a.m. or p.m. milking according to the treatment) and supplemented with grazing pasture during the other half of the day. Milk production was slightly but significantly higher for cows on the TMR treatment (29.1 vs. 28.2 and 27.6). No significant difference between treatments was observed in FCM (27.7 kg/d), and milk fat (3.47) and protein percentage (3.23). While BW change did not differ among treatments (25.7 kg), body condition score increased more in cows fed only a TMR diet (0.14 vs. -0.06 and 0.01). The TMR intake was significantly different between treatments, being highest for cows on the TMR treatment and lowest for cows grazing after the p.m. milking (26.6 vs. 20.3 vs. 17.5 kg/d DM). Income over feed cost differed between treatments, and was approximately 15.3% higher for cows supplemented with high quality pasture during the afternoon compared to cows on TMR. Dairy farmers may obtain economical benefits by practicing this type of management during the grazing season with little effect on milk yield. / Master of Science
72

Effects of Brevibacillus laterosporus and live yeast on rumen fermentation, nutrient digestibility and microbial protein synthesis

Adeleke, Rasaq Ademola 11 1900 (has links)
This study investigated the effects of Brevibacillus laterosporus and live yeast (LY) on rumen fermentation, nutrient digestibility and microbial protein synthesis. The basal diet was a total mixed ration formulated to fulfil the minimum nutrient requirement of early lactating 600 kg Holstein cow producing 40kg of milk with 3.5 % fat and 3.3 % protein using CPM-dairy software (NRC, 2001). Treatments were: T1 (Control: basal diet with no additive), T2 (Basal diet + Brevibacillus laterosporus), T3 (Basal diet + Live yeast), and T4 (Basal diet + Brevibacillus laterosporus + Live yeast). In situ degradation, in vitro batch fermentation were performed. Data obtained were subjected to analysis of variance (ANOVA) using PROC GLM (SAS Institute, 2009). The effective dry matter (DM) degradability evaluated at low (0.02) and medium (0.05) ruminal passage rate (ED1 and ED2) were higher (p<0.05) in T1 compared to T2 and T3, but did not differ (p>0.05) between T2, T3 and T4, and between T1 and T4. When evaluated at fast passage rate (0.08) the effective DM degradability (ED3) was higher (p<0.05) in T1 compared to T3 and T4, but did not differ (p>0.05) between T1 and T2. The difference in ammonia nitrogen production was observed only between T1 and T2, and was higher (p<0.05) in T1. The total VFA’s concentration was higher (p<0.05) in T3 compared to the control. All additives decreased the molar percentage of acetate (P<0.05). The concentration of acetate was lower (p<0.05) in T3 and T4 compared to control. Propionate concentration was higher (p<0.05) in T3 and T4 compared to other treatments and lower (p<0.05) in the control compared to the rest of treatments. Butyrate concentration was higher (p<0.05) in T2 and T4 compared to the rest of the treatments, and lower (p<0.05) in T3 than other treatments. The microbial protein synthesis measured as purine derivate done on residues was higher (p<0.05) for T3 compared to T1 and T2, but did not differ between T1, T2 and T4, and between T3 and T4. These results showed that the two additives have different individual effects on DM and CP degradability, but also associative effects in some fermentation parameters such as propionate production. / Agriculture, Animal Health and Human Ecology / M. Sc. (Agriculture)
73

Getting into the guts of a salty problem : poor animal production from saltbush pastures is due to inefficient rumen fermentation

Mayberry, Dianne January 2009 (has links)
The main hypothesis tested in this thesis was that poor animal production from saltbush pastures is due to the negative effects of high sodium chloride (NaCl) and potassium chloride (KCl) on the ruminal environment, and subsequent effects on microbial populations and products of rumen fermentation. This main hypothesis was tested in two experiments. In the first experiment (Chapter Four) the effects of saltbush and a formulated high-salt diet on the ruminal environment and microbial populations were measured over 24-hours following feeding. Feeding both the saltbush and high-salt diet increased the salinity of the rumen fluid, but the formulated high-salt diet caused a decrease in ruminal pH while the saltbush caused an increase. This resulted in differences in the composition of the ruminal microbial populations between the sheep fed different diets. In the second experiment (Chapter Five) the effects of saltbush and a formulated highsalt diet on rumen fermentation were measured. Sheep fed saltbush had inefficient rumen fermentation and this was only partially explained by the high salt content of the diet. Diets containing high levels of NaCl and KCl provided low levels of net energy to sheep, but sheep fed saltbush lost more energy as methane and faecal energy compared to sheep fed the formulated high-salt diet. Inefficient rumen fermentation could help to explain poor animal production from saltbush pastures. Energy supplements such as barley grain can improve the value of saltbush pastures as feed for sheep, but there is no information on how much supplement is required. A third experiment (Chapter Six) was designed to test the hypothesis that there would be an optimal amount of barley required to improve the efficiency of rumen fermentation in sheep fed saltbush. Barley and straw were combined in a pellet and substituted for saltbush at 0, 20, 40, 60, 80 and 100% of the maintenance ration. Feeding barley and straw improved the efficiency of rumen fermentation in sheep fed saltbush, with an optimal level of supplementation at 60% of the maintenance diet. This is likely to be lower (approximately 20% of maintenance) if barley is fed without straw.
74

Antimicrobial plants of Australia have the potential to prevent lactic acidosis in ruminants

Hutton, Peter January 2008 (has links)
[Truncated abstract] Antimicrobial growth promoters are added to feed to prevent lactic acidosis in ruminant animals by selectively inhibiting rumen bacteria that produce lactic acid. However, recently imposed or impending bans on the use of antimicrobial growth promoters in animal production have lead to a critical need to find practical alternatives that are safe for the animal and consumer and that obtain similar production benefits. I investigated bioactive plants of Australia for their potential to prevent lactic acidosis in ruminants. The unifying hypothesis tested was that plants would be identified that selectively inhibit lactic acid-producing bacteria and consequently protect against lactic acidosis. This hypothesis was tested in a three phase process: phase 1, plant selection and collection; phase 2, a three stage protocol for screening plants and essential oils; phase 3, in vivo experiments and chemical fractionation of the most promising plant. I developed an in vitro bioassay that simulated acidosis by adding glucose to rumen fluid in Bellco tubes and incubating for 5 h (Chapter 4). The pH and gas production were used as indicators of acidosis and fermentation activity. I used this bioassay to screen ninety-five plants (dried and ground material from 79 species) and ten essential oils and included a negative control (oaten chaff) and a positive control (virginiamycin). One plant, Eremophila glabra, produced a similar pH (5.63) to the positive control (5.43) although it inhibited gas production to a moderate extent (P < 0.05). ... Seven serrulatane diterpenes were identified to be the major secondary metabolites in E. glabra. The metabolites were screened using a broth dilution and microtitre spectrophotometry method and were selective against S. bovis at between 320 and 1077 [mu]g/ mL. The serrulatanes from E. glabra were probably responsible for the activity against acidosis that I observed in vitro, because they selectively inhibited lactateproducing bacteria. It is also possible that a synergy between serrulatanes and possibly other metabolites are responsible for the activity observed in vitro. The results from my experiments support the role that bioactive plants may have to replace the antibiotics that are added to livestock feed. Australian plants were identified containing compounds that were active against the bacterial processes responsible for ruminant acidosis. To my knowledge this is the first work undertaken to identify bioactive plants of Australia for their potential to prevent acidosis. I developed in vitro screening bioassays that targeted key indicators of acidosis. These bioassays enabled me to identify 5 plants from the 104 screened that could potentially control acidosis. One of these plants in particular, E. glabra, showed a level of activity in vitro that was comparable to antibiotic protection against acidosis. The exciting in vitro results were not demonstrated in vivo but only one dose level of E. glabra was used, which was based on the in vitro work. In contrast to the in vitro system the rumen is a continuous flow system with greater complexity and it is possible that the concentration of E. glabra that I used in vivo was not optimum. This places importance on future dose response experiments to confirm the efficacy of E. glabra in vivo.

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